Yak Response to High-Altitude Hypoxic Stress by Altering mRNA Expression and DNA Methylation of Hypoxia-Inducible Factors

Xiong, Xianrong; Fu, Mei R.; Lan, Daoliang; Li, Jian; Zi, Xiang-Dong; Zhong, Jincheng

doi:10.1080/10495398.2014.1002563

Cited by 32 publications

(24 citation statements)

References 44 publications

(53 reference statements)

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“…The higher abundance of HIF‐1 α mRNA in cattle from high altitude was on expected line as this is the major transcription factor known to be elevated during hypoxic condition. Similar to our findings, increased mRNA expression of HIF‐1 α was also reported in Yak and Pika (Wang et al, ; Li et al, ; Xiong et al, ) suggesting its crucial role towards high altitude adaptation. The HIF‐1 mRNA gets accumulated under hypoxic conditions and probably an essential adaptive component for the animals living at high altitude.…”

Section: Resultssupporting

confidence: 92%

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Overexpression of genes associated with hypoxia in cattle adapted to Trans Himalayan region of Ladakh

Verma

Sharma

Sodhi

et al. 2018

Cell Biology International

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Ladakh is an important part of the Trans-Himalayan region located between the Kunlun mountain range in the north and the main Great Himalayas to the south in the state of Jammu and Kashmir of India. The local cattle from Leh and Ladakh region, known as "Ladakhi cattle" is a unique germplasm having an excellent adaptation potential to high altitude hypobaric stress. In the present study, an effort was made to evaluate the transcriptional pattern of hypoxia inducing factor-1 (HIF-1) and several of its regulated genes in PBMCs of local Ladakhi cattle, Holstein Frisian crosses, Jersey (exotic) maintained at high altitude region and Sahiwal (Bos indicus) and Karan Fries (cross bred) cattle maintained in tropical environment. The combined data set indicated increased expression of HIF-1 and its regulated genes viz., glucose transporter 1 (GLUT1), vascular endothelial growth factor (VEGF), and hexokinase (HK2) in high altitude cattle indicating their importance in maintaining cellular homeostasis during high altitude hypoxia. The data indicated that hypoxia associated genes accumulated under hypoxic conditions are part of an essential adaptive component for adaptation to the high altitude of the trans-Himalayan region. In contrary, higher expression of molecular chaperons' viz., HSP70 and HSP90 in tropically adapted cattle give tolerance to high ambient temperature prevalent in tropical condition. In conclusion, HIF-1 and its regulatory genes could be termed as important candidates for producing homeostatic responses to hypoxia in cattle populations reared in higher altitudes of the Trans-Himalayan region.

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Section: Resultssupporting

confidence: 92%

“…Expression of hypoxia genes in high altitude native cattle of Ladakh P. Verma et al (Wang et al, 2006;Li et al, 2009;Xiong et al, 2015) suggesting its crucial role towards high altitude adaptation. The HIF-1 mRNA gets accumulated under hypoxic conditions and probably an essential adaptive component for the animals living at high altitude.…”

Section: Resultsmentioning

confidence: 99%

Overexpression of genes associated with hypoxia in cattle adapted to Trans Himalayan region of Ladakh

Verma

Sharma

Sodhi

et al. 2018

Cell Biology International

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“…HIF-1α is degraded by proteasomes under normoxic conditions. In response to hypoxia, however, HIF-1α is transferred into the nucleus and interacts with the HIF-1β subunit to form HIF-1 to initiate or enhance transcription of effector genes, including BNIP3/BNIP3L, insulin-like growth factor II, and vascular endothelial growth factor (Chu and Jones 2016 ; Xiong et al 2015 ).…”

Section: Introductionmentioning

confidence: 99%

HIF-1α/Beclin1-Mediated Autophagy Is Involved in Neuroprotection Induced by Hypoxic Preconditioning

Tan

et al. 2018

J Mol Neurosci

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Hypoxic preconditioning (HPC) exerts a protective effect against hypoxic/ischemic brain injury, and one mechanism explaining this effect may involve the upregulation of hypoxia-inducible factor-1 (HIF-1). Autophagy, an endogenous protective mechanism against hypoxic/ischemic injury, is correlated with the activation of the HIF-1α/Beclin1 signaling pathway. Based on previous studies, we hypothesize that the protective role of HPC may involve autophagy occurring via activation of the HIF-1α/Beclin1 signaling pathway. To test this hypothesis, we evaluated the effects of HPC on oxygen-glucose deprivation/reperfusion (OGD/R)-induced apoptosis and autophagy in SH-SY5Y cells. HPC significantly attenuated OGD/R-induced apoptosis, and this effect was suppressed by the autophagy inhibitor 3-methyladenine and mimicked by the autophagy agonist rapamycin. In control SH-SY5Y cells, HPC upregulated the expression of HIF-1α and downstream molecules such as BNIP3 and Beclin1. Additionally, HPC increased the LC3-II/LC3-I ratio and decreased p62 levels. The increase in the LC3-II/LC3-I ratio was inhibited by the HIF-1α inhibitor YC-1 or by Beclin1-short hairpin RNA (shRNA). In OGD/R-treated SH-SY5Y cells, HPC also upregulated the expression levels of HIF-1α, BNIP3, and Beclin1, as well as the LC3-II/LC3-I ratio. Furthermore, YC-1 or Beclin1-shRNA attenuated the HPC-mediated cell viability in OGD/R-treated cells. Taken together, our results demonstrate that HPC protects SH-SY5Y cells against OGD/R via HIF-1α/Beclin1-regulated autophagy.

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“…Qiu et al (2012) reported that 81 genes detected in yak were related to the hypoxia response and energy metabolism. Hypoxia-inducible factors (HIF-1α and HIF-2α) expressions were higher in yak tissues than in cattle which indicated involvement of HIF-1α and HIF-2α genes in oxygen homeostasis in response to hypoxia (Xiong et al, 2015). The PGM1, PKLR, ATP5F1, and NDUFA10 genes were under positive selection in hypoxia-tolerant Tibetan yak (Tian et al, 2017).…”

Section: Adaptation and Copy Number Variationsmentioning

confidence: 96%

Applications of genomic copy number variations on livestock: A review

Goshu¹,

Chen²,

Yan³

2018

Afr. J. Biotechnol.

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Genomic copy number variations (CNVs) have significantly modified genomic regions during inversions, translocations, duplications and deletions that keep shaping phenotypic variation through changes in gene dosage, regulation and transcript structure. The objective of this review was to discuss the current application of CNVs on domestication, adaptations, productions, coat colour, morphology and diseases of livestock, and also address implication of CNV on genotyping selections, limitation and in the future, directions of livestock breeding. The CNVs are a key role in generating essential variation in the livestock population and disease phenotype. The association of CNVs with phenotypic traits was utilized for the determination of feed conversion ratios in beef cattle, growth, milk production, reproduction, coat color, health, demostication and morphology. Genomic CNVs are an important type of genetic variation in livestock development in different environments. This knowledge will be extremely pertinent from a molecular perspective and practical applications of genomic CNVs in animal breeding and allowed breeders to consider genomic selection of young animals at earlier ages. However, in livestock species, there are still open questions remaining about the genome frequency of CNVs. Therefore, application of genomics CNVs in livestock breaks the bridging between breeders and livestock producers; however, there are still gaps on genotypes and overlapping genes.

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Yak Response to High-Altitude Hypoxic Stress by Altering mRNA Expression and DNA Methylation of Hypoxia-Inducible Factors

Cited by 32 publications

References 44 publications

Overexpression of genes associated with hypoxia in cattle adapted to Trans Himalayan region of Ladakh

Overexpression of genes associated with hypoxia in cattle adapted to Trans Himalayan region of Ladakh

HIF-1α/Beclin1-Mediated Autophagy Is Involved in Neuroprotection Induced by Hypoxic Preconditioning

Applications of genomic copy number variations on livestock: A review

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