Yam Tuber Storage Protein Reduces Plant Oxidants Using the Coupled Reactions as Carbonic Anhydrase and Dehydroascorbate Reductase

Xue, You-Lin; Miyakawa, Takuya; Nakamura, Akira; Hatano, Ken-ichi; Sawano, Yoriko; Tanokura, Masaru

doi:10.1016/j.molp.2015.02.015

Cited by 17 publications

(11 citation statements)

References 9 publications

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“…The H + may participate in easing the reduction of dehydroascorbate to ascorbic acid. Dioscorins, a major tuber storage protein of Yam, functions both as CA and dehydroascorbate reductase (DHAR) [52]. The main reason these proteins are able to facilitate both functions is a His-Asp catalytic dyad.…”

Section: Discussionmentioning

confidence: 99%

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Thiol Redox Regulation of Plant β-Carbonic Anhydrase

et al. 2020

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β-carbonic anhydrases (βCA) accelerate the equilibrium formation between CO2 and carbonate. Two plant βCA isoforms are targeted to the chloroplast and represent abundant proteins in the range of >1% of chloroplast protein. While their function in gas exchange and photosynthesis is well-characterized in carbon concentrating mechanisms of cyanobacteria and plants with C4-photosynthesis, their function in plants with C3-photosynthesis is less clear. The presence of conserved and surface-exposed cysteinyl residues in the βCA-structure urged to the question whether βCA is subject to redox regulation. Activity measurements revealed reductive activation of βCA1, whereas oxidized βCA1 was inactive. Mutation of cysteinyl residues decreased βCA1 activity, in particular C280S, C167S, C230S, and C257S. High concentrations of dithiothreitol or low amounts of reduced thioredoxins (TRXs) activated oxidized βCA1. TRX-y1 and TRX-y2 most efficiently activated βCA1, followed by TRX-f1 and f2 and NADPH-dependent TRX reductase C (NTRC). High light irradiation did not enhance βCA activity in wildtype Arabidopsis, but surprisingly in βca1 knockout plants, indicating light-dependent regulation. The results assign a role of βCA within the thiol redox regulatory network of the chloroplast.

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Section: Discussionmentioning

confidence: 99%

“…Two protonation steps by His 227 would be needed for the formation of ascorbate. At the same time, the released hydroxide would react with carbon dioxide to bicarbonate [52]. But in comparison to the αCAs, the βCA1 has two Asp close to His 227 [8], namely Asp 169, which is part of the zinc-binding pocket and Asp 315.…”

Section: Discussionmentioning

confidence: 99%

Thiol Redox Regulation of Plant β-Carbonic Anhydrase

et al. 2020

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“…Structural studies have shown that dioscorin has a www.nature.com/scientificreports www.nature.com/scientificreports/ Cys28-Cys187 disulfide bond and that its DHA reductase activity is related to its carbonic anhydrase (CA) active center (Fig. 5) 29 . Thus, the DPPH-scavenging activities of YSP may be related to the aromatic amino acids (Trp and Tyr), the CA active center (His 95, His97 and Gln114) and some surface hydrophobic amino acids (Fig.…”

Section: Amino Acid Composition Of Ysp As Shown Inmentioning

confidence: 99%

Recovery of Yam Soluble Protein from Yam Starch Processing Wastewater

Xue

Zhao

Liu

et al. 2020

Sci Rep

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Over the past two decades, many studies have shown that the yam storage protein dioscorin, which is abundant in the wastewater of starch processing, exhibits many biological activities both in vitro and in vivo. In the present study, the acid-precipitation method was optimized using Box-Behnken design (BBD) combined with response surface methodology (RSM) for the recovery of yam soluble protein (YSP) from wastewater. The experimental yield of YSP reached 57.7%. According to relative quantitative proteomics (LC-MS/MS), the crude YSP was mainly composed of 15 dioscorin isoforms, which was further verified by anion-exchange and size-exclusion chromatography. YSP was found to be rich in glutamic acid and aspartic acid, and the eight essential acids made up approximately 33.7% of the YSP. Moreover, the YSP demonstrated antioxidant activity, including scavenging DPPH, hydroxyl and superoxide anion radicals, and the possible structure-activity relationships were discussed. these results indicated that YSp produced by acid precipitation may be used as a protein source with antioxidant properties.

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“…properties (Wu et al, 2015;Capitani et al, 2016). Therefore, in this study we investigated the emulsification properties of DOM which is a potential candidate for Usually harvested in November, Dioscorea opposita is a seasonal crop with a short shelf-life, as it contains protein and steroidal saponins, which reduce the quality of the yam during storage (Yang & Lin, 2008;Xue et al, 2015). Therefore, dried slices of Dioscorea opposita are prepared on an industrial scale.…”

Section: Introductionmentioning

confidence: 99%

Chemical components and emulsification properties of mucilage from Dioscorea opposita Thunb

Zhang

Yao

et al. 2017

Food Chemistry

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The properties of mucilage obtained from Dioscorea opposita, generated during industrial manufacturing were investigated in this study. Characteristics such as monosaccharide content, amino acid content, molecular weight, and structural features were measured, whereas morphology was observed using a scanning/transmission electron microscope. Additionally, emulsification properties at different concentrations (0.2%, 0.4%, 0.6%, 0.8%, and 1.0%) and under acidic and basic pH (5.0 and 9.0) conditions were studied. The results showed that emulsions prepared from mucilage and medium-chain triglycerides presented more effective emulsifying functions and higher stability, especially at low concentrations. Both, acidic and basic conditions improved the overall emulsification properties, which suggested that the isoelectric point of amino acids may be involved in the emulsification properties. The results of this study show that mucilage from Dioscorea opposita can be considered as a sustainable resource of a natural emulsifier obtained from industrial waste.

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Yam Tuber Storage Protein Reduces Plant Oxidants Using the Coupled Reactions as Carbonic Anhydrase and Dehydroascorbate Reductase

Cited by 17 publications

References 9 publications

Thiol Redox Regulation of Plant β-Carbonic Anhydrase

Thiol Redox Regulation of Plant β-Carbonic Anhydrase

Recovery of Yam Soluble Protein from Yam Starch Processing Wastewater

Chemical components and emulsification properties of mucilage from Dioscorea opposita Thunb

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