Yamadazyma siamensis sp. nov., Yamadazyma phyllophila sp. nov. and Yamadazyma paraphyllophila sp. nov., three novel yeast species isolated from phylloplane in Thailand and Taiwan

Abstract

Four strains representing three novel anamorphic yeast species were isolated from the external surface of sugarcane leaves (DMKU-RK254(T)), corn leaves (DMKU-RK548(T)), bean leaves (K129) in Thailand and hengchun pencilwood leaves (TrB1-1(T)) in Taiwan. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene, the internal transcribed spacer (ITS) region, the actin gene (ACT1) and the elongation factor 2 gene (EF2), the four strains were determined to represent novel Yamadazyma species although formation of ascospores was not observed. Strain DMKU-RK254(T) was determined to be related to Candida diddensiae, Candida naeodendra and Candida kanchanaburiensis but with 1.8, 1.8 and 2.0 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene, respectively. It was assigned to Yamadazyma siamensis sp. nov. (type strain DMKU-RK254(T) = BCC 50730(T) = NBRC 108901(T) = CBS 12573(T)). The sequences of the D1/D2 region of the LSU rRNA gene, the ITS region, ACT1 gene and EF2 gene of two strains (DMKU-RK548(T) and K129) were identical but differed from that of strain TrB1-1(T) by 0.6, 1.0, 3.3 and 5.9 % nucleotide substitutions, respectively. Therefore, the two strains (DMKU-RK548(T) and K129) and strain TrB1-1(T) were assigned to be two separate species. The closest species in terms of pairwise sequences similarity of the D1/D2 region to the two novel species was Yamadazyma philogaea but with 1.1-1.7 % nucleotide substitutions. The two strains (DMKU-RK548(T) and K129) were assigned to Yamadazyma phyllophila sp. nov. (type strain DMKU-RK548(T) = BCC 50736(T) = NBRC 108906(T) = CBS 12572(T)) and the strain TrB1-1(T) was named Yamadazyma paraphyllophila sp. nov. (type strain TrB1-1(T) = BCRC 23030(T) = CCTCC AY 204005(T) = CBS 9928(T)).

Yamadazyma endophytica f.a. sp. nov., an ascomycetous yeast species isolated from leaf tissue

“…In addition, several species in this clade have been described from the strains associated with insects Lachance et al, 2011;Jindamorakot et al, 2015) and plant materials (Nisiotou et al, 2010;Lachance et al, 2011;Kaewwichian et al, 2013b;Junyapate et al, 2014;Jindamorakot et al, 2015;Lopes et al, 2015;Wang et al, 2015). In this study, strain DMKU-CE23 T was isolated from corn leaf tissue.…”

“…In the latest edition of The Yeasts, a Taxonomic Study, six ascosporogenous species (Yamadazyma philogaea, Y. akitaensis, Y. mexicana, Y. nakazawae, Y. scolyti, Y. triangularis) and 23 asexual species assigned to the genus Candida were placed in the Yamadazyma clade . Since then, additional species of the genus Candida in this clade have been found Nakase et al, 2008;AmIn et al, 2011;Buddie et al, 2011;Burgaud et al, 2011;Groenewald et al, 2011) and many novel species of the genus Yamadazyma have been discovered and described such as Yamadazyma terventina, Y. siamensis, Y. phyllophila, Y. paraphyllophila, Y. ubonensis, Y. insecticola, Y. epiphylla, Y. riverae, Y. dushanensis and Y. kitorensis (Ciafardini et al, 2013;Kaewwichian et al, 2013b;Junyapate et al, 2014;Jindamorakot et al, 2015;Lopes et al, 2015;Wang et al, 2015;Nagatsuka et al, 2016). In addition, three known species of the genus Candida were transferred to the genus Yamadazyma as novel combinations, namely Yamadazyma olivae, Y. tumulicola and Y. takamatsuzukensis (Nagatsuka et al, 2016).…”

Yamadazyma cocois f.a., sp. nov., an ascomycetous yeast isolated from coconuts

“…ubonensis [5][6][7][8][9][10][11][12][13]. Although, some ideas about re-evaluation of Yamadazyma clade exist [5,12], but currently there are no solid arguments to validate them.…”

Yamadazyma insecticola f.a., sp. nov. and Yamadazyma epiphylla f.a., sp. nov., two novel yeast species

“…Members of the Yamadazyma clade exhibit an association with the gut of beetles and other insects, flowers, tree exudates, plants, mushroom, deep-sea coral, water samples, and marine and mangrove habitats (Nakase et al , 2008; Burgaud et al , 2011; Groenewald et al , 2011; Kurtzman, 2011; Lachance et al , 2011; Kaewwichian et al , 2013; Junyapate et al ., 2014). According to these reports, 20 species in the Yamadazyma clade have been isolated from the gut or frass of insects and 10 species have been obtained from plants.…”

“…Since then, many novel species of the genus Candida in this clade have been described, for example Candida kanchanaburiensis , C. khao-thaluensis , C. vaughaniae , C. tallmaniae , C. oceani , C. sirachaensis , C. sakaeoensis , C. phyllophilla and C. vitiphila (Nakase et al , 2008; Burgaud et al , 2011; Groenewald et al , 2011; Limtong et al ., 2012; Limtong & Kaewwichian, 2013). Recently, five species of the genus Yamadazyma , namely Y. terventina , Y. siamensis , Y. phyllophila , Y. paraphyllophila and Y. ubonensis , were proposed from strains isolated in Italy, Taiwan and Thailand (Ciafardini et al ., 2013; Kaewwichian et al , 2013; Junyapate et al ., 2014). At the time of writing, the Yamadazyma clade consists of 11 species of the genus Yamadazyma and more than 30 species of the genus Candida (Lachance et al , 2011; Kurtzman, 2011).…”