Yangzheng Sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression

Deng, Huimin; Yu, Yaya; Zhou, Aoqiang; Zhu, Jingli; Luo, Lina; Chen, Wanqun; Hu, Ling; Chen, Geng-Xin

doi:10.3748/wjg.v23.i30.5538

Cited by 13 publications

(14 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…miRNAs are widely recognized to play critical roles in diverse cellular processes, and more than half of all human genes have been found to be regulated by miRNAs. Furthermore, numerous therapeutic agents exert anticancer effects by modulating the expression of intracellular miRNAs [ 38 , 49 , 50 ]. Here, through small-RNA sequencing analysis, we identified 57 miRNAs showing >2-fold differential expression following melatonin stimulation, which indicated that some of these miRNAs contribute to the anti-HCC effect of melatonin.…”

Section: Discussionmentioning

confidence: 99%

Melatonin Inhibits the Progression of Hepatocellular Carcinoma through MicroRNA Let7i-3p Mediated RAF1 Reduction

Wang

Yeh

et al. 2018

IJMS

View full text Add to dashboard Cite

Melatonin is the main pineal hormone that relays light/dark-cycle information to the circadian system. Recent studies have examined the intrinsic antitumor activity of melatonin in various cancers, including hepatocellular carcinoma (HCC), the primary life-threatening malignancy in both sexes in Taiwan. However, the detailed regulatory mechanisms underlying melatonin’s anti-HCC activity remain incompletely understood. Here, we investigated the mechanisms by which the anti-HCC activity of melatonin is regulated. Human hepatoma cell lines were treated with 1 and 2 mM melatonin, and functional assays were used to dissect melatonin’s antitumor effect in HCC; small-RNA sequencing was performed to identify the microRNAs (miRNAs) involved in the anti-HCC activity of melatonin; and quantitative RT-PCR and Western blotting were used to elucidate how miRNAs regulate melatonin-mediated HCC suppression. Melatonin treatment at both doses strongly inhibited the proliferation, migration and invasion capacities of Huh7 and HepG2 cell lines, and melatonin treatment markedly induced the expression of the miRNA let7i-3p in cells. Notably, transfection of cells with a let7i-3p mimic drastically reduced RAF1 expression and activation of mitogen-activated protein kinase signaling downstream from RAF1, and rescue-assay results demonstrated that melatonin inhibited HCC progression by modulating let7i-3p-mediated RAF1 suppression. Our findings support the view that melatonin treatment holds considerable promise as a therapy for HCC.

show abstract

Section: Discussionmentioning

confidence: 99%

Melatonin Inhibits the Progression of Hepatocellular Carcinoma through MicroRNA Let7i-3p Mediated RAF1 Reduction

Wang

Yeh

et al. 2018

IJMS

View full text Add to dashboard Cite

show abstract

“…On the contrary, let-7 has target genes [HMGA2 (High Mobility Group AT-Hook 2), IMP-1 (IGF2 mRNA-binding protein 1), LIN28B (Lin-28 homolog B), Ras, and MYC) important for cancer cell stemness [130]. Although let-7a expression was high in GC cell lines, it expression diminished progressively during the progression of gastric mucosa cancerization, confirming that the regulation of let-7a expression may be used as a novel biomarker and molecular mechanism of drug response to treatment [114]. Enhancer of zeste homolog 2 (EZH2) is a histone methyltransferase involved in the silencing of many genes related to cell proliferation and differentiation.…”

Section: Micrornas Regulate Myc Oncogenic Pathwaysmentioning

confidence: 94%

The Complex Network between MYC Oncogene and microRNAs in Gastric Cancer: An Overview

Anauate

Leal

Calcagno

et al. 2020

IJMS

View full text Add to dashboard Cite

Despite the advancements in cancer treatments, gastric cancer is still one of the leading causes of death worldwide. In this context, it is of great interest to discover new and more effective ways of treating this disease. Accumulated evidences have demonstrated the amplification of 8q24.21 region in gastric tumors. Furthermore, this is the region where the widely known MYC oncogene and different microRNAs are located. MYC deregulation is key in tumorigenesis in various types of tissues, once it is associated with cell proliferation, survival, and drug resistance. microRNAs are a class of noncoding RNAs that negatively regulate the protein translation, and which deregulation is related with gastric cancer development. However, little is understood about the interactions between microRNAs and MYC. Here, we overview the MYC role and its relationship with the microRNAs network in gastric cancer aiming to identify potential targets useful to be used in clinic, not only as biomarkers, but also as molecules for development of promising therapies.

show abstract

“…Serum was then stored at −20°C until use. Further details of the protocol are available in two previously published studies ( 22 , 31 ).…”

Section: Methodsmentioning

confidence: 99%

“…RT-qPCR was performed on Axygen 96-well reaction plates with the Applied Biosystems 7500 Real-time PCR System following and the SYBR Premix Ex Taq kit (Takara Bio, Inc.), according to the manufacturer's protocol. The thermocycling conditions were as previously described ( 22 ). According to the instructions of the Bulge-Loop miRNA RT-qPCR Primer, a U6 small nuclear RNA was used as the internal reference.…”

Section: Methodsmentioning

confidence: 99%

“…Yangzheng Sanjie Decoction (YZSJD), a traditional Chinese formula, is composed of Astragali Radix, Scutellariae Barbatae Herba, Arisaematis Rhizoma Preparatum, Citri Sarcodactylis Fructus, Cremastrae Pseudobulbus and Curcumae Longae Rhizoma ( 22 ). In clinical practice, it was revealed that YZSJD is effective to alleviate the symptoms, postpone or even reverse the precancerous lesions (intestinal metaplasia or dysplasia) of GC ( 23 , 24 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Yangzheng Sanjie Decoction-containing serum mediated by microRNA-7 on cell proliferation and apoptosis in gastric cancer

Chen

Nie

et al. 2018

Oncol Lett

Self Cite

View full text Add to dashboard Cite

Gastric cancer (GC) is one of the most common types of cancer and a leading cause of cancer-associated mortality. MicroRNAs (miRNAs/miRs) are demonstrated to function as oncomiRs or tumor-suppressor-miRs in GC. miR-7 has been identified to be a tumor suppressor of GC by targeting epidermal growth factor receptor (EGFR). In our previous study, Yangzheng Sanjie Decoction (YZSJD), a traditional Chinese formula, was identified to be effective in alleviating the symptoms and even postponing turnover of precancerous lesions. To elucidate the mechanism of YZSJD, the present study evaluated the effects of YZSJD of the GC MKN-45 cell line and investigated the underlying molecular mechanisms using YZSJD-containing serum (YCS). The expression of miR-7 in GC, normal and adjacent tissue samples was examined. The results demonstrated that YCS inhibited proliferation by inducing cell cycle arrest at the S phase, and significantly induced apoptosis compared with the control group. miR-7 was significantly downregulated in GC tissues compared with the matched ones. Using reverse transcription-quantitative polymerase chain reaction and western blot analysis, the expression of miR-7 was inversely associated with EGFR. This indicates that YCS inhibits proliferation and induces apoptosis of GC cells mediated by miR-7 targeting EGFR, which may be one of the mechanisms whereby YZSJD exerts its effects on GC.

show abstract

Yangzheng Sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression

Cited by 13 publications

References 35 publications

Melatonin Inhibits the Progression of Hepatocellular Carcinoma through MicroRNA Let7i-3p Mediated RAF1 Reduction

Melatonin Inhibits the Progression of Hepatocellular Carcinoma through MicroRNA Let7i-3p Mediated RAF1 Reduction

The Complex Network between MYC Oncogene and microRNAs in Gastric Cancer: An Overview

Effects of Yangzheng Sanjie Decoction-containing serum mediated by microRNA-7 on cell proliferation and apoptosis in gastric cancer

Contact Info

Product

Resources

About