YAP knockdown inhibits proliferation and induces apoptosis of human prostate cancer DU145 cells

Abstract

The role of yes-associated protein (YAP) in human prostate cancer DU145 cells and its underlying molecular mechanisms were explored in the present study. Initially, the expression levels of YAP were detected in DU145 cells, which revealed that YAP was highly expressed in these cells. To investigate the role of YAP in DU145 cells, a stable YAP‑silenced DU145 cell line was generated using YAP‑small interfering RNA. Reverse transcription‑quantitative polymerase chain reaction and western blotting were performed for mRNA and protein detection, respectively. An MTT assay and flow cytometry were performed to investigate the proliferation and apoptosis of DU145 cells. The results demonstrated that YAP knockdown significantly decreased the proliferative ability of DU145 cells, whereas the percentage of apoptotic cells was markedly increased, compared with the control. In addition, the mRNA and protein expression levels of connective tissue growth factor and cysteine‑rich angiogenic factor 61 were notably decreased, the ratio of B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2‑associated X protein (Bax) was significantly reduced, and the expression levels of caspase 3 were significantly decreased within YAP‑silenced DU145 cells. In conclusion, YAP knockdown reduced the proliferation and induced apoptosis of DU145 cells. Therefore, the gene transcription and protein expression of YAP may be involved in the development of prostate cancer and may be considered a potential target for the treatment of such cancers.

Androgen‐responsive tripartite motif 36 enhances tumor‐suppressive effect by regulating apoptosis‐related pathway in prostate cancer

“…BAX promotes apoptosis by heterodimerizing with Bcl‐2, a known inhibitor of apoptotis . In PC, several signals induce BAX to enhance apoptosis . In the present study, TNFSF10 was also significantly regulated by TRIM36.…”

“…34 In PC, several signals induce BAX to enhance apoptosis. [35][36][37][38] In the present study, TNFSF10…”

<p>Yes-Associated Protein Contributes to Cell Proliferation and Migration of Gastric Cancer via Activation of Gli1</p>

“… 3–5 Ectopic YAP expression was reported in several types of cancers, 6–15 and its overexpression was implicated in promoting tumor cell proliferation, metastasis and EMT. 11 , 12 , 16 In GC, YAP was also showed to be upregulated and involved in the development and progression of GC, 16–19 but the internal mechanism of YAP-mediated regulation of cell proliferation and migration in GC is not well understood and remains to be further explored.…”

“… 3 , 4 In addition, YAP has recently gained much attention in tumor growth and metastasis and is identified as a candidate oncogene. 3–5 YAP protein levels were reported to be frequently overexpressed in multiple types of malignancies including hepatocellular carcinoma, 6 , 7 esophageal cancer, 8 breast cancer, 9 ovarian cancer, 10 prostate cancer, 11 colorectal cancer, 12 , 13 pancreatic cancer 14 and GC. 15 In cancers, overexpression of YAP has been reported to be involved in tumor cell proliferation, metastasis and epithelial–mesenchymal transition (EMT).…”

“… 15 In cancers, overexpression of YAP has been reported to be involved in tumor cell proliferation, metastasis and epithelial–mesenchymal transition (EMT). 11 , 12 , 16 Recent studies showed that YAP was highly expressed in GC and its overexpression exhibits oncogenic property in GC, 16–19 but the precise function and internal mechanisms of YAP in GC remain to be further explored.…”