Yeast dynamin and Ypt6 function in parallel for the endosome‐to‐Golgi retrieval of Snc1

Abstract

Protein recycling is an important cellular process required for cell homeostasis. Results from prior studies have shown that Vps1, a dynamin homologue in yeast, is implicated in protein recycling from the endosome to the trans-Golgi Network (TGN). However, the function of Vps1 in relation to Ypt6, a master GTPase in the recycling pathway, remains unknown. The present study reveals that Vps1 physically interacts with Ypt6 if at least one of them is full-length. We found that overexpression of full-length Vps1, but not GTP hydrolysis-defective Vps1 mutants, is sufficient to rescue abnormal phenotypes of Snc1 distribution provoked by loss of Ypt6, and vice versa. This suggests that Vps1 and Ypt6 function in parallel pathways instead of in a sequential pathway, and that GTP binding/hydrolysis of Vps1 is required for proper traffic of Snc1 toward the TGN. Additionally, we identified two novel Vps1 binding partners, Vti1 and Snc2, which function for the endosome-derived vesicle fusion at the TGN. Taken together, the present study demonstrates that Vps1 plays a role in later stages of the endosome-to-TGN traffic.

Effect of N-acetyl-l-cysteine on Cell Phenotype and Autophagy in Pichia pastoris Expressing Human Serum Albumin and Porcine Follicle-Stimulating Hormone Fusion Protein

“…Thus, autophagy, especially mitophagy and pexophagy, is involved in the promotion of recombinant protein secretion by NAC. In addition, the encoded products of four other genes ( SAR 1 , ARF 1 , SNC 2 , COG 4 ) play important roles in vesicular trafficking associated with the Golgi apparatus [ 29 , 30 , 31 ].…”

“…Another Rab GTPase, Vps 21 mediates CORVET from trans-Golgi Network (TGN) to early endosomes by binding to the CORVET complex. Moreover, Snc2 functions for the endosome-derived vesicular fusion at the TGN [ 30 ], and Cog 4 is a member of COG complex located on Golgi, which responses to transport vesicle from endosome to cis Golgi [ 31 ]. The reduced expression level of SAR1 , ARF1 , SNC2, VPS21, and COG4 genes indicated that NAC affects vesicular transport among Golgi, endosome, and ER.…”

Action of Arl1 GTPase and golgin Imh1 in Ypt6-independent retrograde transport from endosomes to thetrans-Golgi network

“…, 2001; Siniossoglou and Pelham, 2001; Luo and Gallwitz, 2003; Suda et al. , 2013; Makaraci et al. , 2018), Rab6, the mammalian homologue of Ypt6, was shown to be involved in the secretory pathway from Golgi to the plasma membrane (Grigoriev et al.…”

Vesicle trafficking in Arabidopsis pollen tubes

“…Golgi is a central station for the classification and processing, especially glycosylation, of proteins within the endomembrane system. In animals and yeast, small GTPases Rab6/Ypt6 mediates the fusion of vesicles from endosomes at the Golgi apparatus through the Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex [48][49][50]. Arabidopsis homologs of Ypt6 are RabH1A-E [51].…”