Yeast TOR (DRR) proteins: amino-acid sequence alignment and identification of structural motifs

Cafferkey, Robert; McLaughlin, Megan M.; Young, Peter R.; Johnson, Randall K.; Livi, George P.

doi:10.1016/0378-1119(94)90141-4

Cited by 55 publications

(37 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1). Many Crm1p substrates contain a leucine-rich nuclear export sequence (L- 9 were incubated with either GST-Srp1p or GST bound to glutathione-agarose beads. GST fusion proteins and bound materials were separated by SDS-PAGE and detected by Western blotting with anti-MYC mAb 9E10.…”

Section: Discussionmentioning

confidence: 99%

“…Input, one-fifth of the total input of Gln3p-MYC 9 used to bind to GST or GST-Srp1p. b, GST and GST-Srp1p fusion protein used for binding to Gln3p-MYC 9 . Proteins were separated on SDS-polyacrylamide gel and stained with Coomassie Blue.…”

Section: Discussionmentioning

confidence: 99%

“…The fragment was inserted into BamHI-digested pGEX-3x, such that the coding sequence for GST was in frame with the second codon of SRP1. To generate pRS315-GLN3-MYC 9 and pRS416-GLN3-MYC 9 , the chromosomal gene GLN3-MYC 9 and its natural promoter were generated by polymerase chain reaction from genomic DNA prepared from the GLN3-MYC 9 strain (17) and cloned into pRS315 (LEU CEN) or pRS416 (URA CEN) using the SalI and NotI restriction sites. The resultant plasmids were transformed into yeast and shown to express Gln3p-MYC 9 at a level comparable with that of the chromosomal GLN3-MYC 9 .…”

Section: Yeast Strains and Plasmids-all Of The Yeast Strains Are Showmentioning

confidence: 99%

“…TOR is the yeast target of rapamycin (7)(8)(9)(10)(11)(12) and a key player of nutrient-mediated signal transduction (recently reviewed in Refs. [13][14][15][16].…”

mentioning

confidence: 99%

See 3 more Smart Citations

Phosphorylation Regulates the Interaction between Gln3p and the Nuclear Import Factor Srp1p

Carvalho

Bertram

Wente

et al. 2001

Journal of Biological Chemistry

View full text Add to dashboard Cite

Gln3p is a GATA-type transcription activator of nitrogen catabolite repressible (NCR) genes. Gln3p was recently found to be hyperphosphorylated in a TOR-dependent manner and resides in the cytoplasm in high quality nitrogen. In contrast, during nitrogen starvation or rapamycin treatment, Gln3p becomes rapidly dephosphorylated and accumulates in the nucleus, thereby activating nitrogen catabolite repression genes. However, a detailed mechanistic understanding is lacking for the regulation of Gln3p nucleocytoplasmic distribution. In this study, we applied a functional genomics approach to identify the nuclear transport factors for Gln3p. We found that yeast karyopherin ␣/Srp1p and Crm1p are required for the nuclear import and export of Gln3p, respectively. Similarly, the Ran GTPase pathway is also involved in the nuclear translocation of Gln3p. Finally, we show that Srp1p preferentially interacts with the hypophosphorylated versus the hyperphosphorylated Gln3p. These findings define a possible mechanism for regulated nucleocytoplasmic transport of Gln3p by phosphorylation in vivo.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Yeast Strains and Plasmids-all Of The Yeast Strains Are Showmentioning

confidence: 99%

“…TOR is the yeast target of rapamycin (7)(8)(9)(10)(11)(12) and a key player of nutrient-mediated signal transduction (recently reviewed in Refs. [13][14][15][16].…”

mentioning

confidence: 99%

See 2 more Smart Citations

Phosphorylation Regulates the Interaction between Gln3p and the Nuclear Import Factor Srp1p

Carvalho

Bertram

Wente

et al. 2001

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…The molecular cloning of PIKK-encoding cDNAs began with the isolation of the products of the target of rapamycin genes (TOR1 and TOR2) from Saccharomyces cerevisiae (Cafferkey et al 1994;Helliwell et al 1994), followed by the cloning of the mammalian ortholog, termed mTOR (also called FRAP or RAFT1) (Brown et al 1994;Sabatini et al 1994;Sabers et al 1995). As the TOR acronym indicates, these PIKK family members are the protein targets of the potent antifungal and immunosuppressive agent, rapamycin (for reviews, see Abraham and Wiederrecht 1996;Gingras et al 2001).…”

Section: The Pikk Familymentioning

confidence: 99%

Cell cycle checkpoint signaling through the ATM and ATR kinases

Abraham¹

2001

Genes Dev.

1,833

1,797

View full text Add to dashboard Cite

Growing Yeast for Fun and Profit: Use of Saccharomyces Cerevisiae as a Model System in Drug Discovery

Ross‐Macdonald

2003

Model Organisms in Drug Discovery

View full text Add to dashboard Cite

Yeast has great utility as a surrogate system to study aspects of mammalian biology. This utility extends to the drug discovery process, where yeast has been used to reveal the mechanism of action of compounds, to discover and characterize components of signaling pathways and to dissect protein function. These applications of yeast are illustrated by examples of research published by major pharmaceutical companies.

show abstract

Yeast TOR (DRR) proteins: amino-acid sequence alignment and identification of structural motifs

Cited by 55 publications

References 16 publications

Phosphorylation Regulates the Interaction between Gln3p and the Nuclear Import Factor Srp1p

Phosphorylation Regulates the Interaction between Gln3p and the Nuclear Import Factor Srp1p

Cell cycle checkpoint signaling through the ATM and ATR kinases

Growing Yeast for Fun and Profit: Use of Saccharomyces Cerevisiae as a Model System in Drug Discovery

Contact Info

Product

Resources

About