Yellow‐b, ‐c, ‐e and ‐h are required for normal body coloration in <i>Henosepilachna vigintioctopunctata</i>

Wang, Pei; Ze, Long-Ji; Jin, Lin; Li, Guoqing

doi:10.1002/arch.21856

Cited by 7 publications

(4 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the wood tiger moth, yellow affects the conversion of DOPA into black dopamine melanin (Galarza, 2021). Yellow-e in particular has been linked to larval colouration in Bombyx mori (Ito et al ., 2010) and adult colour in beetles (Wang et al ., 2022), while another gene, yellow-f , has a role in eumelanin production (Barek et al ., 2018).…”

Section: Discussionmentioning

confidence: 99%

Colour polymorphism associated with a gene duplication in male wood tiger moths

Brien

Orteu

Yen

et al. 2022

Preprint

View full text Add to dashboard Cite

Colour is often used as an aposematic warning signal, with predator learning expected to lead to a single colour pattern within a population. However, there are many puzzling cases where aposematic signals are also polymorphic. The wood tiger moth, Arctia plantaginis, uses bright hindwing colours as a signal of unpalatability, and males have discrete colour morphs which vary in frequency geographically. In Finland, both white and yellow morphs can be found, and these colour morphs also differ in behavioural and life-history traits. Complex polymorphisms such as these are often explained by supergenes. Here, we show that male colour is linked to an extra copy of a yellow family gene that is only present in the white morphs. This white-specific duplication, which we name valkea, is highly upregulated during wing development, and could act to reduce recombination, thus potentially representing a supergene. We also characterise the pigments responsible for yellow, white and black colouration, showing that yellow is partly produced by pheomelanins, while black is dopamine-derived eumelanin. The yellow family genes have been linked to melanin synthesis and behavioural traits in other insect species. Our results add to only a few examples of seemingly paradoxical and complex polymorphisms which are associated with single genes.

show abstract

Section: Discussionmentioning

confidence: 99%

Colour polymorphism associated with a gene duplication in male wood tiger moths

Brien

Orteu

Yen

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Secondly, sensory setae are located on the larval mouthparts and legs, the pupal thorax, and the adult mouthparts and elytra. Furthermore, H. vigintioctopunctata is sensitive to RNA interference (RNAi) [ 10 , 12 , 13 , 14 , 15 ]. This offers an ideal insect model to investigate the roles of Dyl throughout the entire lifecycle.…”

Section: Introductionmentioning

confidence: 99%

Dusky-like Is Critical for Morphogenesis of the Cellular Protuberances and Formation of the Cuticle in Henosepilachna vigintioctopunctata

Zhang

Tan

Lin

et al. 2023

Biology

Self Cite

View full text Add to dashboard Cite

Dusky-like (Dyl) is a transmembrane protein containing a zona pellucida domain. Its physiological roles during metamorphosis have been well explored in Drosophila melanogaster and have also been documented in Tribolium castaneum. However, Dyl has undergone a functional shift between Diptera and Coleoptera insects. Further investigation of Dyl in other insects will be helpful to further clarify its function in insect growth and development. Henosepilachna vigintioctopunctata is an important Coleoptera that causes enormous economic losses in agriculture in China. In this study, we found that the expression of Hvdyl was detectable in embryos, larvae, prepupae, pupae, and adults. We knocked down Hvdyl in third- and fourth-instar larvae and pupae with RNA interference (RNAi). RNAi of Hvdyl mainly caused two phenotypic defects. Firstly, the growth of epidermal cellular protuberances was suppressed. Injection of dsdyl (double-stranded dusky-like RNA) at the third-instar larval stage truncated the scoli throughout the thorax and abdomen and shortened the setae on the head capsules and mouthparts of the fourth-instar larvae. Introduction of dsdyl at the third- and fourth-instar stages led to misshapen pupal setae. The setae were shortened or became black nodules. Treatment with dsdyl at the larval and pupal stages resulted in deformed adults with completely suppressed wing hairs. Moreover, the knockdown of Hvdyl at the third-instar stage caused deformed larval mouthparts at the fourth-instar period. As a result, foliage consumption was inhibited, and larval growth was slowed. The results indicate that Dyl is associated with the growth of cellular protuberances throughout development and with the formation of the cuticle in H. vigintioctopunctata.

show abstract

“…DEG, differentially expressed genes; mRNA, messenger RNA; qRT-PCR, quantitative real-time polymerase chain reaction; SEM, standard error of the mean.fatbody. For example, yellow-FA and TH were significantly upregulated in the fatbody of BmIDGF mutants; both genes are involved in cuticle pigmentation in larvae, pupae, and adults(Wang et al, 2022a(Wang et al, , 2022b. Differences in the expression of melanin synthesis-related genes, including yellow and TH, lead to specific color pattern diversity in several Drosophila species(Wang et al, 2022b;Wittkopp et al, 2003;Zhang et al, 2019).…”

mentioning

confidence: 99%

“…For example, yellow-FA and TH were significantly upregulated in the fatbody of BmIDGF mutants; both genes are involved in cuticle pigmentation in larvae, pupae, and adults(Wang et al, 2022a(Wang et al, , 2022b. Differences in the expression of melanin synthesis-related genes, including yellow and TH, lead to specific color pattern diversity in several Drosophila species(Wang et al, 2022b;Wittkopp et al, 2003;Zhang et al, 2019). L-dopa, a major tyrosineF I G U R E 6 Combinatorial metabolomic and transcriptomic analysis.…”

mentioning

confidence: 99%