Yersinia Enterocolitica and Yesinia Enterocolitica‐like Species: Their Pathogenicity and Significance in Foods

Zink, Donald L.; Lachica, R. V. F.; Dubel, J.

doi:10.1111/j.1745-4565.1982.tb00447.x

Cited by 23 publications

(15 citation statements)

References 75 publications

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“…1,14 Investigaciones anteriores llevadas a cabo en varios países, han confirmado también la existencia de la bacteria en tonsilas de cerdos; 1 y a partir de muestras de lengua y canales frescas. 8 Los serotipos O:3 y O:9 inclusive se han identificado a partir de lengua, garganta, tonsilas, canales, carne, jamón y contenido cecal de cerdos.…”

Section: Discussionunclassified

Identificación y tipificación de biotipos y serotipos de Yersinia enterocolitica

et al. 2001

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OBJETIVO: Conocer la existencia de Yersinia enterocolitica en suínos visiblemente sanos y sacrificados para el consumo humano. MÉTODOS: Fueron estudiadas 100 muestras de tejido linfático obtenidas en el momento del sacrifício, en un matadero del Estado de México. Fueron realizados muestreos pilotos de 20 casos, de los cuales 20% fueron positivos, permitiendo obtener una muestra estudiada (n=100). Las muestras colectadas de tejido linfático fueron acondicionadas para el aislamiento de Yersinia enterocolitica en caldo de Rappaport y en medio de cultivo de Salmonella-Shigella y MacConkey. Las identificaciones fueron efectuadas por medio de pruebas bioquímicas y serológicas, utilizándose en el caso los antisueros O:3, O:8 y O:9 para la biotipificación correspondiente. RESULTADOS: Fueron obtenidos 22 aislamientos tipificándose 8 serotipos pertenecientes al O:3 y 8 al O:9 correspondientes al biotipo 1; y, en 6 muestras no fue posible la serotipificación. No se encontró en el total de los aislados el serotipo O:8. CONCLUSIONES: En base en la metodología, se registró la presencia de Y. enterocolitica y sus serogrupos en tejido linfático de porcinos por la primera vez en México; esto es importante porque el patógeno y sus serotipos aislados están comprometidos con mayor frecuencia con problemas de salud pública.

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Section: Discussionunclassified

Identificación y tipificación de biotipos y serotipos de Yersinia enterocolitica

et al. 2001

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Section: Introductionmentioning

confidence: 99%

“…Occasional dissemination of these organisms into the blood, liver, and spleen gives rise to enteric fever. Pathogenicity or virulence in these species is a complex interplay between ecology, geographic distribution, biochemical and antigenic properties, and chromosomal and plasmid-encoded genes (Zink et al 1982). Y. pseudotuberculosis and Y. enterocolitica can survive in most natural environments or in food matrices by means of highly adaptable metabolic pathways that are typical of free-living enterobacteria (Rosso et al 2008).…”

Section: Introductionmentioning

confidence: 99%

EFFECTS OF ORANGE JUICE pH ON SURVIVAL, UREASE ACTIVITY AND DNA PROFILES OF YERSINIA ENTEROCOLITICA AND YERSINIA PSEUDOTUBERCULOSIS STORED AT 4C

Abdela¹,

Graham²,

Habtemariam

et al. 2011

Journal of Food Safety

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The objective of this study was to determine the survival, growth rate and possible cellular adaptation mechanisms of Y. pseudotuberculosis and Y. enterocolitica in orange juice under different pH conditions. Yersinia was inoculated in orange juice with adjusted pH levels of 3.9, 4.0, and 7.0 and stored at 4 C for 3, 24, 72 and 168 hours (h). The inter-and intra-species variation is significant to the pH and time of incubation variables (p<0.05). At 3.9 pH the CFU (colony forming units) count decreased significantly. At pH 3.9 and 4.0, Y. enterocolitica and Y. pseudotuberculosis survived for at least 30 days and 15 days, respectively. Yersinia that survived under low pH in orange juice revealed enhanced urease activity within 12 h of incubation. The attachment gene (ail) could not be detected by PCR in Y. enterocolitica from undiluted sample incubated for 24 h or longer. Moreover, the FesI-restriction profile was altered when Y. pseudotuberculosis was stored at pH 4.0 orange juice for 7 days. These results indicate that Yersinia could survive and grow at low pH and the survival mechanisms could also enable the bacteria to survive the stomach pH barrier to cause enteric infection.

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“…It is caused by pathogenic strains of Yersinia enterocolitica, which can be distinguished from non-pathogenic strains by in vivo virulence tests in animals, or on the basis of their serotype and biotype (Bottone 1977;Wauters 1981 ;Cornelis et al 1987;Wauters et al 1987). As animal tests are less desirable and iable (Zink et al 1982;Cornelis et al 1987;Miller et al 1989), alternative methods to distinguish between pathogenic and non-pathogenic strains have been sought.…”

Section: Introductionmentioning

confidence: 99%

Digoxigenin‐labelled inv‐ and ail‐probes for the detection and identification of pathogenic Yersinia enterocolitica in clinical specimens and naturally contaminated pig samples

Goverde

Jansen

Brunings

et al. 1993

Journal of Applied Bacteriology

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A non-radioactive colony hybridization method was developed for the rapid detection of Yersinia enterocolitica in primary isolates and for differentiation between pathogenic and non-pathogenic strains. The method is based on, respectively, the presence of the inv-locus in all Yersinia spp. and the presence of the ail-gene in pathogenic Y. enterocolitica only. Hybridization results with ail-probes of 132 strains of Y. enterocolitica were in good agreement with pathogenicity phenotypes as indicated by a tissue culture invasion (TCI) assay and by serotyping. All TCI+ strains and only two TCI- strains were positive by hybridization with ail. Hybridization results with inv- or ail-probes of 150 primary isolates of human, animal or slaughterhouse origin were compared with those of conventional methods to detect and identify Y. enterocolitica. All samples that were positive for Yersinia spp. by cultivation (four of 66) or were positive for pathogenic Y. enterocolitica by cultivation and serotyping (six of 84) were also positive by hybridization with, respectively, the inv- or ail-probe. In three slaughterhouse swab samples, in which Yersinia spp. were not detected by cultivation (2%), strong positive hybridization signals were obtained with the inv- and/or ail-probe. Four other swab samples which were negative by cultivation produced weak positive signals by hybridization with inv- and/or ail-probes. These results indicate that the method can be used for (1) the identification of pathogenic Y. enterocolitica isolates and (2) the detection of Yersinia spp. in primary isolates of naturally contaminated samples.

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Yersinia Enterocolitica and Yesinia Enterocolitica‐like Species: Their Pathogenicity and Significance in Foods

Cited by 23 publications

References 75 publications

Identificación y tipificación de biotipos y serotipos de Yersinia enterocolitica

Identificación y tipificación de biotipos y serotipos de Yersinia enterocolitica

EFFECTS OF ORANGE JUICE pH ON SURVIVAL, UREASE ACTIVITY AND DNA PROFILES OF YERSINIA ENTEROCOLITICA AND YERSINIA PSEUDOTUBERCULOSIS STORED AT 4C

Digoxigenin‐labelled inv‐ and ail‐probes for the detection and identification of pathogenic Yersinia enterocolitica in clinical specimens and naturally contaminated pig samples

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