YestroSens, a field-portable S. cerevisiae biosensor device for the detection of endocrine-disrupting chemicals: Reliability and stability

Lobsiger, Nadine; Venetz, Jonathan E.; Gregorini, Michele; Christen, Matthias; Christen, Beat; Stark, Wendelin J.

doi:10.1016/j.bios.2019.111710

Cited by 14 publications

(9 citation statements)

References 38 publications

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“…There is an urgent need for the development and application of tools for detecting and quantifying these micropollutants in the environment, with biosensor technology emerging as a potential resource for this purpose. These biosensors detect molecules by means of biological components such as enzymes, DNA, antibodies and aptamers with which they interact, forming measurable electrochemical and optical signals (Lobsiger et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking water

et al. 2020

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The chemical and pharmaceutical industries are the main generators of residues, such as phenolic microcontaminants, including catechol, resorcinol, p-nitrophenol and 4-chlorophenol. Therefore, this work aims to identify these microcontaminants through an emerging contaminant biosensor by means of an enzymatic biosensor constructed with an enzymatic extract from the fungus Marasmiellus colocasiae. Based on the differential pulse voltammetry electrochemical technique, the biosensor was used to analyze the patterns of catechol, resorcinol, p-nitrophenol and 4-chlorophenol. The analysis of a sample prepared with these standards in water from the public supply network was also carried out. As a result, it was possible to verify that the biosensor developed in this study is more sensitive than conventional methods and has a greater affinity for catechol. In the sample prepared with the standards, it was possible to qualitatively identify the presence of 4-chlorophenol, resorcinol and catechol. The proposed biosensor was sensitive and has potential for application in the analysis of microcontaminants in the environment with the detection limit = 0.17 µmol L-1, and the quantification limit = 0.52 µmol L-1.

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Section: Introductionmentioning

confidence: 99%

Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking water

et al. 2020

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“…Sensitive optical biosensors for EDC determination (LOD 0.08 nM for 17β‐estradiol) have been developed at a laboratory stage together with two prototypes (Cevenini et al, 2018 ; Lobsiger et al, 2019 ; Lopreside et al, 2019 ; Pham et al, 2012 , 2013 ). In this case, the ability of yeast cells to correctly express transfected vertebrate nuclear receptors and the consequent generation of transgenic yeast strains represents an advantage with respect to bacterial sensors.…”

Section: Yeast‐based Biosensorsmentioning

confidence: 99%

Biological and technical challenges for implementation of yeast‐based biosensors

Wahid

Ocheja

Marsili

et al. 2022

Microbial Biotechnology

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Biosensors are low‐cost and low‐maintenance alternatives to conventional analytical techniques for biomedical, industrial and environmental applications. Biosensors based on whole microorganisms can be genetically engineered to attain high sensitivity and specificity for the detection of selected analytes. While bacteria‐based biosensors have been extensively reported, there is a recent interest in yeast‐based biosensors, combining the microbial with the eukaryotic advantages, including possession of specific receptors, stability and high robustness. Here, we describe recently reported yeast‐based biosensors highlighting their biological and technical features together with their status of development, that is, laboratory or prototype. Notably, most yeast‐based biosensors are still in the early developmental stage, with only a few prototypes tested for real applications. Open challenges, including systematic use of advanced molecular and biotechnological tools, bioprospecting, and implementation of yeast‐based biosensors in electrochemical setup, are discussed to find possible solutions for overcoming bottlenecks and promote real‐world application of yeast‐based biosensors.

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“…Many contaminants or indicators can be measured through yeast-based biosensors, such as pharmaceutical and personal care products (PPCPs), methyl methanesulfonate, formaldehyde, reactive oxidative species inducers, biological oxygen demand (BOD) and doxycycline (Miller et al, 2020a). For example, Saccharomyces cerevisiae (fungus) is an efficient candidate host associated with specific inducible promotors (e.g., CUP1p and copper) for the toxicity of phenol, PPCPs, estradiol, pesticides, and BOD (Gao et al, 2016, Costa et al, 2018, Lobsiger et al, 2019, Miller et al, 2020a, Miller et al, 2020b. Environmental factors, especially temperature, significantly influence the viability of yeast biosensors, that control the yeast activities and affect the stability of the biosensors (Jarque et al, 2016).…”

Section: Yeastsmentioning

confidence: 99%

Progress of optical biosensors for analyzing pathogens and organic pollutants in water since 2015

et al. 2022

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Water contamination by pathogens and organic pollutants is one of the major environmental problems that risk human health. Climate change with extreme weather can promote their prevalence in waters. Environmental monitoring of these pollutants in a fast, continuous, and accurate manner is of increasing demand, especially under the climate change context, but is challenged by their ubiquity and trace concentrations. Optical biosensing is one of the desired solutions owing to its rapid and accurate detection with high sensitivity. Principally, an optical biosensor recognizes these bioactive toxins and contaminants by tailored bioreceptors (e.g., aptamer, enzyme, and cells) and transduces the biological response to optical signals. Research efforts have been made on tailoring bioreceptors and enhancing signal transducing by nanoparticles. This study comprehensively reviewed the mechanisms of optical biosensing and the recent development of bioreceptors and nanomaterials on the enhancement for the rapid, easy, and accurate analysis of emerging contaminants in water. The advantages and challenges on sensitivity, selectivity, and durability of biosensors were discussed along with the opportunities and development strategies.

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YestroSens, a field-portable S. cerevisiae biosensor device for the detection of endocrine-disrupting chemicals: Reliability and stability

Cited by 14 publications

References 38 publications

Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking water

Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking water

Biological and technical challenges for implementation of yeast‐based biosensors

Progress of optical biosensors for analyzing pathogens and organic pollutants in water since 2015

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