Yi-qi-yang-yin-tian-sui-fang enhances cisplatin-induced tumor eradication and inhibits interleukin-7 reduction in non-small cell lung cancer

Abstract

Traditional Chinese Medicine (TCM) has been recognized to be conducive to enhancing the efficiency and reducing the side effects in the whole course of cancer treatment. The mechanisms of TCM/chemotherapy combination involved with interleukin-7 (IL-7) potentially enhance immune responses against tumor. In the present study, we emphasized on a herbal formulation Yi-qi-yang-yin-tian-sui-fang or TCM for short, and investigated its roles in chemotherapy in non-small cell lung cancer (NSCLC). The mice bared with tumor were treated with cisplatin (DDP) and simultaneously administrated with/without low, medium and high doses of TCMs (effective content: 0.5, 2.0 and 8.0 g/per mice) via oral gavage. The results indicated that combination of TCM further elevated the therapy efficiency of DDP in a dose-dependent manner. The growth of tumor cells was estimated by Ki-67 stain and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) assay. The addition of TCM to the DDP treatment could significantly decrease the expression of Ki-67 and promote the apoptosis of tumor cells. In addition, the serum IL-7 level was down-regulated by DDP but restored by the treatment of TCM. The expression of IL-7 and its receptor IL-7R in tumor tissues was also recovered by TCM. Furthermore, the side effect from bone marrow suppression (myelosuppression) induced by DDP were assessed. TCM could abrogate DDP-induced apoptosis of bone marrow and also remarkably induced the expressions of IL-7 and hematopoietic growth factors including G-CSF, GM-CSF, SCF, and SDF-1 in bone marrow. These data indicated that this TCM combined with DDP showed superior anti-tumor effects with reduced myelosuppression via up-regulating IL-7.

Effect of Yisui Multipurpose Soup’s Amelioration on D-Galactose-Induced Neuronal Cells

“…The preparation of blank serum was the same as that of drug-containing serum preparation, but rats were administered the same amount of normal saline. The serum of the same group was merged, inactivated at 56°C for 30 min, filtered by using a 0.22 μ m microporous membrane, and stored at −20°C for later use [ 17 ].…”

Integrating Network Pharmacology and Experimental Validation to Elucidate the Mechanism of Yiqi Yangyin Decoction in Suppressing Non‐Small‐Cell Lung Cancer

“…Clinically, YYD was found to enhance the apatinib sensitivity in mid-advanced NSCLC [ 17 ]. YYD also promoted cisplatin-induced tumor eradication and suppressed interleukin-7 reduction in NSCLC [ 18 ]. However, the biological functions and pharmacological mechanisms of YYD in inhibiting NSCLC have not been comprehensively investigated with appropriate methods.…”

Xiaoyan granule plus aptinib in patients with advanced gastric cancer who failed radiotherapy and chemotherapy: A prospective, phase II trial

“…Meanwhile, with the continuous elucidation of the anti-tumor mechanism of traditional Chinese medicine (TCM), various clinical data demonstrated that TCM had the advantages of multiple targets, multiple pathways, and multiple layers 8 . TCM has been recognized to be conducive to enhancing e ciency and safety, especially having signi cant impacts on killing cancer cells, decreasing side effects of chemoradiotherapy, and thereby prolonging the overall survival time of patients 9,10 At present, there is a lack of evaluation on the e cacy and safety of TCM combined with apatinib in the treatment of AGC. Therefore, the study aimed to evaluate the effectiveness and safety of XYG combined with aptinib in the treatment of AGC who failed radiotherapy and chemotherapy.…”