Yield coefficients in dependence on milieu conditions and cell states II. Influence of perturbations on continuous cultivation of the yeast Lodderomyces elongisporus on hydrocarbons

Abstract

The influence of perturbations on continuous yeast cell cultivations on hydrocarbons of crude oil is described.Due to such perturbations the fermentation system leaves its steady state, passes through transitional stages and reaches a new steady state. In the transitional stages a damped oscillation of biomass concentration, percentage of budding cells, oxygen consumption rate, and heat flow is observed. These results can be explained by partial synchronization of yeast cell functions in microorganism populations. I n phases of partial synchronization the cell cycle of a single cell is reflected by the behaviour of the microorganism population. The adaptationof the carbon substrate feeding rate to the state of partially synchronized microorganism population makes possible lower specific substrate consumption in biomass production.

Candida maltosa

“…• under the influence of perturbations, like substrate or oxygen limitations, which occur in industrial recirculation reactors used for SCP production on alkanes, as well as under the influence of alternating milieu changes as substrate (glucose, sucrose, or n-alkanes) and oxygen supply during cultivation in laboratory or industrial fermenters (Glombitza and Heinritz 1979;Heinritz and Bley 1979;Heinritz et al 1981Heinritz et al , 1985Glombitza 1982;Riege et al 1989;Zentgraf 1991aZentgraf ,b,c, 1993);…”

“…Other species names formerly used in the literature for different C. maltosa strains are given with the respective references. Additionally, in some cases publications are given since the strains were renamed into C. Heinritz et al (1981Heinritz et al ( , 1983a,b) Germany (or IMET H128) Voigt (1981,1984) and Schneider and Triems (1981) Stichel et al (1981Stichel et al ( , 1982) (PCK) Schwedt, Germany Wiinsche (1981, 1983) Wiinsche et al (1981) Mauersberger et al (1981, 1984) Riege et al (1981) Glombitza (1982) Gradova et al (1983) Schneider et al (1983) Miiller et al (1982) Brendler et al (1983,b) Voigt et al (1984a,b, 1985) Blasig et al (1984) Bayer et al (1985) Heinritz et al (1985 Candida maltosa Bode andBirnbaum (1984) Golubev et al (1986) Candida maltosa H or H62 Candida Metz and Reuter (1977) Triebel et al (1980) Grirnmecke and Reuter (1980) Briickner and Troger (1981a,b) Kolblin and Birkenbeil (1981) Grirnrnecke and Reuter (1981a,b,c,d) Grirnmecke et al (1981) Kolblin and Troger (1982) Fischer and Reuter (1982) Niiske et al (1982) Rober and Reuter (1982) Candida maltosa H Rober and Reuter (1984a) Candida maltosa 14 Candida maltosa L4 Kunze et al (1984b) Abbreviations: -not determined; C. maltosa -Candida maltosa; C. tropicalis -Candida tropicalis; P. guilliermondii -Pichia guilliermondii; 1. elongisporus -Loddero...…”

Yield coefficients in dependence on milieu conditions and cell states III. Induction of synchrony in continuous yeast cell cultivation by milieu changes

“…Jn our former papers (BLEY et al. 1980al. , HEINRITZ et al 1981) the dependence of specific yield coefficients on milieu conditions and cell states in discontinuous and continuous cultivations of a yeast on various carbon substrates was reported.…”