557Grass-clover mixtures combine the advantages of both their components; their yield usually exceeds those of the components grown in monocultures (Annicchiarico and Tomasoni 2010). Moreover, the production of dry matter throughout the year is balanced; grasses yield more in spring whereas clover crops produce more dry matter in summer (Mooso and Wedin 1990). Clover crops have a higher content of protein than grass (Evans et al. 1996, Gökkus et al. 1999); yet, their duration in the mixture is lower as compared to grass (Hejduk and Knot 2010). More erect leaves of grasses and horizontal leaves of clover crops minimize any interspecific competition (Lantinga et al. 1999).Another advantage of grass-clover mixture is a possibility of lower input of nitrogen fertilizer due to atmospheric nitrogen fixation by root-nodule bacteria at the roots of clover crops (Erkovan et al. 2008); yet, to obtain the maximum yield of dry matter, it is necessary to fertilize the grass-clover stands with nitrogen. At the conventional surface application of nitrogen fertilizers, the plants, both grass and clover, take up nitrogen mainly in the nitrate form. At the same time, clover, similar to other legumes fertilized with mineral nitrogen, produces nodules as a prerequisite of N 2 fixation. Nodule formation and therefore N 2 fixation may be reduced when N is supplied as mineral ferti-
ABSTRACTIn a small-plot trial, grass hybrids Perseus (loloid) and Felina (festucoid) were compared in a mixture with red clover (Trifolium pratense L.) and their reactions to surface application of nitrogen fertilizers or injection into soil according to CULTAN method were investigated. Both fertilizer application methods were used with three levels of nitrogen. Mixtures containing the Felina hybrid had higher yields (12.1 t/ha) compared to the Perseus hybrid (11.0 t/ha), and they also showed higher clover abundance. Increasing dosage of nitrogen resulted in slightly higher yield, whereas the trend in clover abundance was opposite. Nitrogen injection resulted in slightly higher dry matter yields and slightly higher clover abundance as compared to surface fertilization. The N content in the grass-clover mixture was balanced for both types of fertilizer application and did not change significantly with increasing dosage of fertilizer. A slightly higher N content was observed at the Felina hybrid treatments; the dependence of this element on clover abundance in the mixture was set up to 50%.