Yogurt ContainingLactobacillus gasseriOLL 2716 (LG21 yogurt) Accelerated the Healing of Acetic Acid-Induced Gastric Ulcer in Rats

Abstract

We have reported that LG21 yogurt containing Lactobacillus gasseri OLL 2716 (LG21 yogurt) inhibits the formation of HCl-induced acute gastric lesions through the generation of prostaglandin E 2 . This study aimed to determine the role of viable Lactobacillus in the healing of acetic acid-induced chronic gastric ulcer.LG21 yogurt or -ray radiated LG21 yogurt was administered orally twice a day for 10 d at a dose of 5 ml/kg. LG21 yogurt significantly accelerated the healing of the ulcer, but -ray radiated LG21 yogurt did not. However, both yogurts significantly inhibited HCl-induced gastric erosive lesions and enhanced the generation of gastric mucosal prostaglandin E 2 . From the above results, it was found that viable bacteria are needed to accelerate the healing of chronic gastric ulcer, but not to inhibit gastric lesions. 2) and that LG21 yogurt exerted gastroprotective action against acute gastric lesions and antral ulcers through the generation of prostaglandin in rats.3) However, it is not clear whether LG21 yogurt is effective in the healing of pre-existing chronic gastric ulcers.Elliott et al. 4) have reported that suppression of colonization of gram-negative bacteria with antibiotics resulted in marked acceleration of ulcer healing, and that induction of Lactobacillus colonization showed marked acceleration of ulcer healing, where the ulcers were induced with acetic acid in rats. Moreover, Brzozowski et al. 5) reported that Candida albicans inoculation delayed the healing of acetic acid-induced gastric ulcers in rats, followed by a fall in gastric mucosal blood flow and a rise in plasma gastrin levels, and that these effects were significantly attenuated by co-treatment with Lactobacillus. These findings suggest the usefulness of probiotics in ulcer healing.In this study, we compared the effects of LG21 yogurt with the effects of -ray radiated LG21 yogurt on the healing of acetic acid-induced gastric ulcers in rats. Moreover, to compare the efficacy for chronic gastric ulcer with that for acute gastric lesions, HCl-induced acute gastric lesion model was also used. Material and MethodsAnimals. Male Sprague-Dawley rats weighing about 200 g were purchased from SLC (Shizuoka, Japan) and kept for 1 week in a room whose temperature and humidity were kept at 21 AE 2 C and 55 AE 15%. The animal studies were performed according to the Guiding Principles for the Care and Use of Laboratory Animals approved by the Japanese Pharmacological Society.Chronic gastric ulcer induction by acetic acid. Chronic gastric ulcers were induced by the method proposed by Okabe et al., 6) with slight modifications. In brief under pentobarbital anesthesia, the stomach was exposed after the midline laparotomy of the abdomen, and a round plastic mold (6 mm in diameter) was placed tightly on the anterior serosal surface of the stomach between the fundus and the corpus. Gracious acetic acid at 100 ml was poured into the mold and allowed to remain on the serosal surface. After 1 min, the excess acetic acid was removed and the serosa was...

The Probiotic Mixture VSL#3 Accelerates Gastric Ulcer Healing by Stimulating Vascular Endothelial Growth Factor

“…Curiously, one study [28] demonstrated that bacterial colonization occurs rapidly after ulcer induction and in particular, colonization of certain bacteria including Lactobacillus could significantly improves gastric ulcer healing. Other studies have shown that probiotic bacteria Lactobacillus species, Saccharomyces boulardii , Escherichia coli strain Nissle 1917 are effective in preventing and treating acute gastric damage associated with acetic acid [12], stress [13], aspirin [29], ethanol [30], ibuprofen [31] and indomethacin [32] induced gastric ulcers. In humans, circumstantial evidence suggests that regular ingestion of a Lactobacillus -containing product protects the integrity of the gastric mucosal barrier against indomethacin [32], while others [15] have reported that the effect of using probiotic bacteria along with triple therapy is no better than using triple therapy alone for treatment of gastric ulcers.…”

“…As VSL#3 was administered as a live cell suspension, this formulation could have exerted its protective effects as live microbes, by their metabolites, cell wall components and/or DNA content or all of the above. We have not performed an experiment to compare the effect of live microbes and heat inactivated probiotic mixture to assess this question even though previous studies have shown that heat-killed or radiation inactivated probiotic bacteria (in a dose similar to that of live microbes) failed to promote ulcer-healing [11], [12]. Further, since we did not assessed angiogenesis, the inference that VSL#3 is promoting gastric ulcer healing through angiogenesis is only speculated based on VSL#3 ability to induce a robust up regulation of VEGF, which is critically important in angiogenesis [35].…”

“…However, treatment with ample quantity of multiple probiotic microorganisms may overcome this limitation. There are a few reports of individual probiotic bacteria including Lactobacillus rhamnosus [11], L. gasseri [12], L. acidophilus [13] and Bifidobacterium [14] conferring protection against gastric ulcers in promoting ulcer healing in different in vivo ulcer models. However, studies on the effect of probiotic bacteria on enhancing H. pylori eradication were inconsistent in their findings [14], [15].…”

Fermented fish oil suppresses T helper 1/2 cell response in a mouse model of atopic dermatitis via generation of CD4+CD25+Foxp3+ T cells

“…Recent studies have investigated the anti-allergic effects of fermented food products such as yogurt, cheese, and soybean paste on a variety of allergic reactions, diabetes and some cancers [28-30]. Fermentation can transmute the chemical structure of some constituents to create new substances.…”

The Effect of Helicobacter pylori Eradication on the Gastrointestinal Microbiota in Patients with Duodenal Ulcer

“…As reported, the probiotic mixture VSL#3 (including Lactobacilli, Bifidobacteria and Streptococcus species) healed acetic acid induced GU in rats by increasing the expression and production of angiogenesis promoting growth factors, primarily vascular endothelial growth factor [25]. And Lactobacillus gasseri OLL 2716 could accelerate the healing of GU, which significantly influenced gastric erosive lesions and enhanced the generation of gastric mucosal prostaglandin E2 [26]. Similar to our results, after H. pylori eradication, Lactobacillus acidophilus were recolonized in the antrum and corpus.…”