Yolk protein endocytosis by oocytes in Drosophila melanogaster: immunofluorescent localization of clathrin, adaptin and the yolk protein receptor

Richard, David; Gilbert, Matthew K.; Crum, B.; Hollinshead, Debra M; Schelble, Suzanne; Scheswohl, Danielle M

doi:10.1016/s0022-1910(00)00165-7

Cited by 56 publications

(35 citation statements)

References 32 publications

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“…YPs are transferred into oocytes from follicular cells in early vitellogenesis and from the fat body in late vitellogenesis. In the latter case, YPs are secreted into hemolymph and deposited in nursing cells to be then delivered to oocytes through circular channels [33]. Richard et al [10] have confirmed JH-dependent stimulation of ecdysteroid synthesis in the D. melanogaster ovaries: biosynthesis of ecdysteroids increases when ovaries obtained from young females (in the first 18 h after eclosion) are incubated with JH bisepoxide-3.…”

Section: Discussionmentioning

confidence: 98%

The ecdysoneless 1 Gene Regulates Metabolism of the Juvenile Hormone and Dopamine in Drosophila melanogaster

2005

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The dopamine (DA) content and the level of juvenile hormone (JH) degradation were studied in females of the wild-type Canton S strain and the ecdysoneless 1 ( ecd 1 ) mutant, which does not produce ecdysone at a restrictive temperature (29 ° C). Exposure at the restrictive temperature considerably increased the JHhydrolyzing activity and the DA content in five-day ecd 1 females compared with flies of both strains growing at 19 ° C and Canton S females exposed at 29 ° C. In one-day ecd 1 females, the level of JH degradation also increased at the restrictive temperature, but the DA content was low. The effect of ecdysone deficiency on the stress reaction in Drosophila melanogaster females was studied using changes in DA content and JH degradation as the reaction indicators. The ecd 1 mutation did not prevent the initiation of the stress reaction in females exposed at the restrictive temperature, but changed its intensity (stress reactivity). The interaction of 20-hydroxyecdysone with JH and DA in regulating Drosophila reproduction under normal conditions and in stress is discussed. GENERAL GENETICS

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Section: Discussionmentioning

confidence: 98%

The ecdysoneless 1 Gene Regulates Metabolism of the Juvenile Hormone and Dopamine in Drosophila melanogaster

2005

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“…The YP receptor in D. melanogaster was identified and cloned by taking advantage of the yolkless (yl) mutation (Schonbaum et al, 1995), and is referred to as the yolkless protein, Yl. Despite their unrelated ligands, the Yl shares high sequence identity with insect VgRs (Sappington et al, 1996;Sappington and Raikhel, 2004), and polyclonal antibodies raised to A. aegypti VgR recognize the D. melanogaster Yl (Richard et al, 2001) indicating similar tertiary structures. Dantuma et al (1999) cloned the L. migratoria Lp receptor (LpR) and determined that it is a member of the LDL receptor (LDLR) family.…”

Section: General Characteristics Of Receptormediated Endocytosismentioning

confidence: 99%

“…Two splice variants of LpR mRNA have been identified that give rise to AaLpRfb and AaLpRov, which are specific for the fat body and the ovary, respectively (Seo et al, 2003). In addition to recognizing Yl, anti-Aedes VgR polyclonal antibodies recognize an abundant 80 kDa protein in D. melanogaster ovaries, which possibly represents an ovarian LpR, in addition to Yl (Richard et al, 2001). Genes encoding putative LpRs also have been identified in the Drosophila genome (Culi and Mann, 2003).…”

Section: General Characteristics Of Receptormediated Endocytosismentioning

confidence: 99%

Vitellogenesis and Post-Vitellogenic Maturation of the Insect Ovarian Follicle

Swevers

2005

Comprehensive Molecular Insect Science

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“…Yolk proteins are the sole nutritional source for Drosophila embryogenesis. They are synthesized in follicle cells and fat bodies, and are taken up by the oocytes through endocytosis during Drosophila oogenesis (Compagnon et al, 2009;Morrison et al, 2008;Richard et al, 2001). Drosophila oogenesis can be divided into 14 stages involving a process of egg chamber growth and maturation.…”

Section: Introductionmentioning

confidence: 99%

Endophilin B is required for the Drosophila oocyte to endocytose yolk downstream of Oskar

et al. 2014

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The nutritional environment is crucial for Drosophila oogenesis in terms of controlling hormonal conditions that regulate yolk production and the progress of vitellogenesis. Here, we discovered that Drosophila Endophilin B (D-EndoB), a member of the endophilin family, is required for yolk endocytosis as it regulates membrane dynamics in developing egg chambers. Loss of D-EndoB leads to yolk content reduction, similar to that seen in yolkless mutants, and also causes poor fecundity. In addition, mutant egg chambers exhibit an arrest at the previtellogenic stage. D-EndoB displayed a crescent localization at the oocyte posterior pole in an Oskar-dependent manner; however, it did not contribute to pole plasm assembly. DEndoB was found to partially colocalize with Long Oskar and Yolkless at the endocytic membranes in ultrastructure analysis. Using an FM4-64 dye incorporation assay, D-EndoB was also found to promote endocytosis in the oocyte. When expressing the full-length D-endoB FL or D-endoB ∆SH3 mutant transgenes in oocytes, the blockage of vitellogenesis and the defect in fecundity in D-endoB mutants was restored. By contrast, a truncated N-BAR domain of the D-EndoB only partially rescued these defects. Taken together, these results allow us to conclude that D-EndoB contributes to the endocytic activity downstream of Oskar by facilitating membrane dynamics through its N-BAR domain in the yolk uptake process, thereby leading to normal progression of vitellogenesis.

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Yolk protein endocytosis by oocytes in Drosophila melanogaster: immunofluorescent localization of clathrin, adaptin and the yolk protein receptor

Cited by 56 publications

References 32 publications

The ecdysoneless 1 Gene Regulates Metabolism of the Juvenile Hormone and Dopamine in Drosophila melanogaster

The ecdysoneless 1 Gene Regulates Metabolism of the Juvenile Hormone and Dopamine in Drosophila melanogaster

Vitellogenesis and Post-Vitellogenic Maturation of the Insect Ovarian Follicle

Endophilin B is required for the Drosophila oocyte to endocytose yolk downstream of Oskar

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