Untitled

Abstract

Cefprozil, a new oral cephalosporin, consists of a 90:10 cis:trans isomer mixture. Sensitive, specific and reproducible high performance liquid chromatographic methods have been developed for the simultaneous quantification of the two stereoisomers of cefprozil in plasma and urine samples from human and rats. Cephalexin acted as the internal standard. Plasma protein was precipitated with acetonitrile and trichloracetic acid with subsequent extraction of acetonitrile. After vortexing and centrifuging, the aqueous phase was injected onto a reverse phase C8 column. Urine samples were acidified with sodium acetate buffer (pH 3.8) and then directly injected onto a reverse phase C18 column. The detector was set at 280 nm. These methods were applied to determine protein binding of both isomers in human and rat sera, and to perform a pharmacokinetic study in human. Results showed that both isomers bound moderately to serum proteins with no interference by the other isomer. The pharmacokinetic study in human indicated that cefprozil was well absorbed and the cis and trans isomers have similar pharmacokinetics.

Flow-injection chemiluminescent determination of cefprozil using Tris (2,2′-bipyridyl) ruthenium (II)-permanganate system

“…The most methods of analysis of the drug in bulk form or in its pharmaceutical formulations rely on the use of high-performance liquid chromatography (HPLC) methods. Few techniques have been reported to determine cefprozil either in pure form, pharmaceutical preparations or biological fluids; including colorimetry [3][4][5], UV spectrophotometry [6] and HPLC [7][8][9]. Abdel Sattar et al [3] described a spectrophotometric method for the determination of cefprozil.…”

HPLC method for simultaneous determination of cefprozil diastereomers in human plasma

“…Since both isomers exhibit antimicrobial activities [9], it is necessary to determine the pharmacokinetics of each isomer separately. A bioassay has been developed for the determination and quantification of cefprozil diastereomers separately [10]. Although this assay suggested a highly specific and precise bioanalytical method, there were some limitations such as lack of stability and validation data.…”

HPLC determination of cefprozil in tablets using monolithic and C18 silica columns

“…The explanation of this improvement was associated to the very low noise of the assays (about 0.25–0.50 mAU), which was interpreted as a total result of acetonitrile usage in mobile phase, high absorptivity of CPZ at 200 nm and avoiding use of buffers to maintain pH. In addition, literature search shows that 245 nm 31, 254 nm 24, and 280 nm 19, 20, 22, 25, 28–30 wavelengths were mainly preferred in UV‐based detections of CPZ. Method development studies exhibited that CPZ absorbs UV light about 79% less at 245 nm, 81% less at 254 nm, and 70% less at 280 nm when compared with 200 nm (Fig.…”

“…Being mostly focused on bioavailability and bioequivalency, the reported assays describe quantitative determination of CPZ in biological fluids such as breast milk 19, plasma 20, and middle ear fluid 21, 22, and in some pharmaceutical preparations such as tablets 23, 24 and oral suspensions 25. Within the methods used to determine CPZ, LC with MS 26–28 or UV 19, 20, 22, 24–25, 28–31 detection was popular. Among these, MS detection shows its superiority over UV detection in terms of specificity and detection limits because of unique capabilities such as multiple and selected reaction monitoring.…”