Zds2p Regulates Swe1p-dependent Polarized Cell Growth in<i>Saccharomyces cerevisiae</i>via a Novel Cdc55p Interaction Domain

Yasutis, Kimberly; Vignali, Marissa; Ryder, Matthew R.; Tameire, Feven; Dighe, Shubha; Fields, Stanley; Kozminski, Keith G.

doi:10.1091/mbc.e10-04-0326

Cited by 28 publications

(56 citation statements)

References 87 publications

(130 reference statements)

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“…The proteins Zds1 and Zds2, which associate with and regulate PP2A Cdc55 (Yasutis et al 2010;Wicky et al 2011) help tip the kinase-phosphatase balance in favor of Cdk1 (Queralt and Uhlmann 2008b). They probably accomplish this by binding and retaining PP2A Cdc55 in the cytoplasm, thereby lowering its nuclear concentration (Rossio and Yoshida 2011), but the precise mechanism remains unclear.…”

Section: Fear Pathwaymentioning

confidence: 99%

Mitotic Exit and Separation of Mother and Daughter Cells

2012

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Productive cell proliferation involves efficient and accurate splitting of the dividing cell into two separate entities. This orderly process reflects coordination of diverse cytological events by regulatory systems that drive the cell from mitosis into G1. In the budding yeast Saccharomyces cerevisiae, separation of mother and daughter cells involves coordinated actomyosin ring contraction and septum synthesis, followed by septum destruction. These events occur in precise and rapid sequence once chromosomes are segregated and are linked with spindle organization and mitotic progress by intricate cell cycle control machinery. Additionally, critical parts of the mother/daughter separation process are asymmetric, reflecting a form of fate specification that occurs in every cell division. This chapter describes central events of budding yeast cell separation, as well as the control pathways that integrate them and link them with the cell cycle.

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Section: Fear Pathwaymentioning

confidence: 99%

Mitotic Exit and Separation of Mother and Daughter Cells

2012

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“…4 Among them, the regulatory B subunit is largely responsible for the subcellular localization and substrate specificity of PP2A. 4 In budding yeast, cells lacking Cdc55 (a regulatory B subunit) exhibit multiple cell cycle defects, including mitotic entry, [5][6][7][8][9][10] spindle assembly checkpoint (SAC), 5,[11][12][13] and precocious mitotic exit. 12,[14][15][16][17][18] We have proposed previously that PP2A-Cdc55 activity is largely controlled by its subcellular distribution.…”

Section: Introductionmentioning

confidence: 99%

Comparative genetic analysis of PP2A-Cdc55 regulators in budding yeast

et al. 2014

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“…Cdc55 localizes to various sites, including the bud cortex, the bud neck, the vacuolar membrane, and the nucleus (28). Localization of PP2A-Cdc55 is regulated by tethering to the cytoplasmic anchor proteins Zds1 and Zds2 (29,30). Cortical and cytoplasmic localization of Cdc55 requires Zds1/2, and Cdc55 accumulates in the nucleus in the absence of Zds1/2 (31).…”

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confidence: 99%

Yeast Protein Phosphatase 2A-Cdc55 Regulates the Transcriptional Response to Hyperosmolarity Stress by Regulating Msn2 and Msn4 Chromatin Recruitment

Reiter

Klopf

Wever

et al. 2013

Molecular and Cellular Biology

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We have identified Cdc55, a regulatory B subunit of protein phosphatase 2A (PP2A), as an essential activating factor for stress gene transcription in Saccharomyces cerevisiae. The presence of PP2A-Cdc55 is required for full activation of the environmental stress response mediated by the transcription factors Msn2 and Msn4. We show that PP2A-Cdc55 contributes to sustained nuclear accumulation of Msn2 and Msn4 during hyperosmolarity stress. PP2A-Cdc55 also enhances Msn2-dependent transactivation, required for extended chromatin recruitment of the transcription factor. We analyzed a possible direct regulatory role for PP2A-Cdc55 on the phosphorylation status of Msn2. Detailed mass spectrometric and genetic analysis of Msn2 showed that stress exposure causes immediate transient dephosphorylation of Msn2 which is not dependent on PP2A-Cdc55 activity. Furthermore, the Hog1 mitogen-activated protein kinase pathway activity is not influenced by PP2A-Cdc55. We therefore propose that the PP2A-Cdc55 phosphatase is not involved in cytosolic stress signal perception but is involved in a specific intranuclear mechanism to regulate Msn2 and Msn4 nuclear accumulation and chromatin association under stress conditions.

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Zds2p Regulates Swe1p-dependent Polarized Cell Growth inSaccharomyces cerevisiaevia a Novel Cdc55p Interaction Domain

Cited by 28 publications

References 87 publications

Mitotic Exit and Separation of Mother and Daughter Cells

Mitotic Exit and Separation of Mother and Daughter Cells

Comparative genetic analysis of PP2A-Cdc55 regulators in budding yeast

Yeast Protein Phosphatase 2A-Cdc55 Regulates the Transcriptional Response to Hyperosmolarity Stress by Regulating Msn2 and Msn4 Chromatin Recruitment

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