Zebrafish (<i>Danio rerio</i>) as a Model for Understanding the Process of Caudal Fin Regeneration

Lebedeva, L.P.; Zhumabayeva, Beibitgul; Gebauer, Tatyana; Kisselev, Ilya; Aitasheva, Zaure

doi:10.1089/zeb.2020.1926

Cited by 15 publications

(4 citation statements)

References 153 publications

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“…Recently, zebrafish, a nonmammalian vertebrate, has become one of the top disease models for investigation because the similarity of its genome reaches as high as 70% with that of humans [ 33 ]. It can regenerate its heart [ 34 , 35 ], retina [ 36 ], kidney [ 37 , 38 ], liver [ 39 , 40 ], and appendants, for instance, the fins [ [41] , [42] , [43] ], throughout its entire life, which makes zebrafish one of the most popular models for studying tissue regeneration. Among regenerable organs, the tail fin, which is well organized with vessels, nerves, and stromal structures and comprises skin, mesenchyme, and bone frames, has become a popular model for studying tissue regeneration due to its repeatability, ease of manipulation, and timesaving [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

Turning gray selenium into a nanoaccelerator of tissue regeneration by PEG modification

Cao

Zhang

et al. 2022

Bioactive Materials

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Section: Resultsmentioning

confidence: 99%

Turning gray selenium into a nanoaccelerator of tissue regeneration by PEG modification

Cao

Zhang

et al. 2022

Bioactive Materials

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“…Oxidative stress and inflammation are well known to be key factors initiating regenerative processes in mammals and zebrafish ( Schäfer and Werner, 2008 ; Koh and DiPietro, 2011 ; Dunnill et al, 2017 ; Nguyen-Chi et al, 2017 ; Paredes et al, 2021 ). Since caudal fin amputation in zebrafish has become a valuable model for better understanding tissue regenerative processes ( Lebedeva et al, 2020 ), we decided to use this model to monitor the potential regenerative properties of H. lanceolatum .…”

Section: Resultsmentioning

confidence: 99%

Hypericum lanceolatum Lam. Medicinal Plant: Potential Toxicity and Therapeutic Effects Based on a Zebrafish Model

et al. 2022

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Hypericum lanceolatum Lam. (H. lanceolatum) is a traditional medicinal plant from Reunion Island used for its pleiotropic effects mainly related to its antioxidant activity. The present work aimed to 1) determine the potential toxicity of the plant aqueous extract in vivo and 2) investigate its putative biological properties using several zebrafish models of oxidative stress, regeneration, estrogenicity, neurogenesis and metabolic disorders. First, we characterized the polyphenolic composition by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and identified chlorogenic acid isomers, quercetin and kaempferol derivatives as the major compounds. We then evaluated for the first time the toxicity of an aqueous extract of H. lanceolatum and determined a maximum non-toxic concentration (MNTC) in zebrafish eleutheroembryos from 0 to 96 hpf following OECD (Organization for Economic Cooperation and Development) guidelines. This MNTC test was also determined on hatched eleutheroembryos after 2 days of treatment (from 3 to 5 dpf). In our study, the anti-estrogenic effects of H. lanceolatum are supported by the data from the EASZY assay. In a tail amputation model, we showed that H. lanceolatum at its MNTC displays antioxidant properties, favors immune cell recruitment and tissue regeneration. Our results also highlighted its beneficial effects in metabolic disorders. Indeed, H. lanceolatum efficiently reduces lipid accumulation and body mass index in overfed larva- and adult-models, respectively. In addition, we show that H. lanceolatum did not improve fasting blood glucose levels in a hyperglycemic zebrafish model but surprisingly inhibited neurogenesis impairment observed in diabetic conditions. In conclusion, our study highlights the antioxidant, pro-regenerative, anti-lipid accumulation and pro-neurogenic effects of H. lanceolatum in vivo and supports the use of this traditional medicinal plant as a potential alternative in the prevention and/or treatment of metabolic disorders.

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“…However, many questions regarding caudal fin regeneration in zebrafish are still unanswered. Previous studies on caudal fin regeneration in zebrafish have mostly focused on coding genes [ 34 ]. Little attention has been paid to ncRNAs, especially lncRNAs.…”

Section: Discussionmentioning

confidence: 99%

The Expression and Function of lincRNA-154324 and the Adjoining Protein-Coding Gene vmp1 in the Caudal Fin Regeneration of Zebrafish

Wen

Zhang

et al. 2022

IJMS

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Caudal fin regeneration is regulated by a variety of mechanisms, but the role of long non-coding RNA (lncRNA) has rarely been studied. The present study aimed to describe the landscape of lncRNAs during caudal fin regeneration using whole transcriptome sequencing, and then to conduct a functional study on the target lncRNAs using real-time fluorescent quantitative PCR (RT-qPCR), in situ hybridization, and the CRISPR/Cas9 method for lncRNA gene knockout. The results of the transcriptome sequencing showed that a total of 381 lncRNAs were differentially expressed, among which ENSDART00000154324 (lincRNA-154324) was found to be highly related to caudal fin regeneration, and thus it was chosen as the target lncRNA for the subsequent functional study. The results regarding the temporal and spatial expression of lincRNA-154324 and the gene knockout results from CRISPR/Cas9 indicated that lincRNA-154324 is involved in the caudal fin regeneration of zebrafish. Importantly, we serendipitously discovered that the cis correlation coefficient between lincRNA-154324 and its neighboring gene vacuole membrane protein 1 (vmp1) is extremely high, and they are essential for the process of caudal fin regeneration. Moreover, studies have found that vmp1 plays an important role in protein secretion, organelle formation, multicellular development, and autophagy. Collectively, our result may provide a framework for the identification and analysis of lncRNAs involved in the regeneration of the zebrafish caudal fin.

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Zebrafish (Danio rerio) as a Model for Understanding the Process of Caudal Fin Regeneration

Cited by 15 publications

References 153 publications

Turning gray selenium into a nanoaccelerator of tissue regeneration by PEG modification

Turning gray selenium into a nanoaccelerator of tissue regeneration by PEG modification

Hypericum lanceolatum Lam. Medicinal Plant: Potential Toxicity and Therapeutic Effects Based on a Zebrafish Model

The Expression and Function of lincRNA-154324 and the Adjoining Protein-Coding Gene vmp1 in the Caudal Fin Regeneration of Zebrafish

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