Zebrafish in Endocrine Systems: Recent Advances and Implications for Human Disease

Löhr, Heiko; Hammerschmidt, Matthias

doi:10.1146/annurev-physiol-012110-142320

Cited by 167 publications

(139 citation statements)

References 185 publications

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“…This suggests that it may not be the drastic changes, but the relative ratios of the secreted signaling molecules in combination in the pituitary gland that are associated with the transition from the juvenile to the sexual maturity stage. All the master endocrine glands and orthologs for nearly all the mammalian endocrine peptides have been identified in teleosts (Zohar et al 2010, Kim et al 2011, Lohr & Hammerschmidt 2011. However, the functional and anatomical correlations between many teleost and mammalian endocrine signals are still unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Each of the pituitary hormones is released into the bloodstream and acts on different target organs to strictly regulate a variety of biological activities, thereby maintaining physiological homeostasis, growth, and reproduction (Lohr & Hammerschmidt 2011, Perez-Castro et al 2012. Many of these peptide hormones are synthesized as inactive precursors, which when converted to their mature forms by proteolytic enzymes generate a large diversity of bioactive proteins and peptides.…”

Section: Introductionmentioning

confidence: 99%

“…Despite the vast emergence of available information, very little work has been carried out to evaluate zebrafish as a model species for somatic growth studies, including applications to aquaculture (Lohr & Hammerschmidt 2011). Zebrafish is phylogenetically closer to the farmed cyprinid fish species than any other vertebrate model, which makes it a useful organism to study the gene functions of aquaculture fish.…”

Section: Introductionmentioning

confidence: 99%

“…Somatic growth is directly regulated by the endocrine somatotropic axis, where Gh functions as a major secreted component within the pituitary gland (Lohr & Hammerschmidt 2011, Perez-Castro et al 2012. To understand the expression and production of hormones by the pituitary gland, orchestrated by changes in physiological homeostasis during the growth burst stage and the adult slow somatic growth stage, in this study, we dissected pituitaries of zebrafish at 45 and 90 dpf and carried out a global transcriptome analysis on them using the RNA-seq technique.…”

Section: Introductionmentioning

confidence: 99%

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Zebrafish pituitary gene expression before and after sexual maturation

He¹,

Dai²,

Chen³

et al. 2014

Journal of Endocrinology

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Sexual maturation and somatic growth cessation are associated with adolescent development, which is precisely controlled by interconnected neuroendocrine regulatory pathways in the endogenous endocrine system. The pituitary gland is one of the key regulators of the endocrine system. By analyzing the RNA sequencing (RNA-seq) transcriptome before and after sexual maturation, in this study, we characterized the global gene expression patterns in zebrafish pituitaries at 45 and 90 days post-fertilization (dpf). A total of 15 043 annotated genes were expressed in the pituitary tissue, 3072 of which were differentially expressed with a greater than or equal to twofold change between pituitaries at 45 and 90 dpf. In the pituitary transcriptome, the most abundant transcript was gh. The expression levels of gh remained high even after sexual maturation at 90 dpf. Among the eight major pituitary hormone genes, lhb was the only gene that exhibited a significant change in its expression levels between 45 and 90 dpf. Significant changes in the pituitary transcripts included genes involved in the regulation of immune responses, bone metabolism, and hormone secretion processes during the juvenile-sexual maturity transition. Real-time quantitative PCR analysis was carried out to verify the RNA-seq transcriptome results and demonstrated that the expression patterns of the eight major pituitary hormone genes did not exhibit a significant gender difference at 90 dpf. For the first time, we report the quantitative global gene expression patterns at the juvenile and sexual maturity stages. These expression patterns may account for the dynamic neuroendocrine regulation observed in body metabolism.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Zebrafish pituitary gene expression before and after sexual maturation

He¹,

Dai²,

Chen³

et al. 2014

Journal of Endocrinology

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“…(b) Selected features of the turquoise killifish that are conserved in human and vertebrate canonical research organisms (zebrafish and house mouse), but are missing in invertebrate canonical research organisms (round worm and fruit fly). References: nervous system: Shimeld and Holland (2000), Freeman and Doherty (2006); Lohr and Hammerschmidt (2011), Oikonomou and Shaham (2011), immune system: Langenau and Zon (2005), Engelmann and Pujol (2010), Buchon, Silverman and Cherry ( 2014); circulatory system: Lehmacher, Abeln and Paululat ( 2012); Stephenson, Adams and Vaccarezza ( 2017); respiratory system: Schottenfeld, Song and Ghabrial ( 2010); skeletal system: Shimeld and Holland (2000); muscular system: Moerman and Williams (2006), Demontis, Piccirillo, Goldberg and Perrimon ( 2013), Piccirillo, Demontis, Perrimon and Goldberg ( 2014), Goody, Carter, Kilroy, Maves and Henry ( 2017); digestive system: McKay, McKay, Avery and Graff ( 2003), Arrese and Soulages (2010), Hashmi et al. ( 2013), Kuraishi, Hori and Kurata ( 2013), Lemaitre and Miguel‐Aliaga (2013), McGhee (2013), Ritter et al.…”

Section: Research Organisms For Agingmentioning

confidence: 99%

The African turquoise killifish: A research organism to study vertebrate aging and diapause

Brunet²

2018

Aging Cell

135

118

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SummaryThe African turquoise killifish has recently gained significant traction as a new research organism in the aging field. Our understanding of aging has strongly benefited from canonical research organisms—yeast, C. elegans, Drosophila, zebrafish, and mice. Many characteristics that are essential to understand aging—for example, the adaptive immune system or the hypothalamo‐pituitary axis—are only present in vertebrates (zebrafish and mice). However, zebrafish and mice live more than 3 years and their relatively long lifespans are not compatible with high‐throughput studies. Therefore, the turquoise killifish, a vertebrate with a naturally compressed lifespan of only 4–6 months, fills an essential gap to understand aging. With a recently developed genomic and genetic toolkit, the turquoise killifish not only provides practical advantages for lifespan and longitudinal experiments, but also allows more systematic characterizations of the interplay between genetics and environment during vertebrate aging. Interestingly, the turquoise killifish can also enter a long‐term dormant state during development called diapause. Killifish embryos in diapause already have some organs and tissues, and they can last in this state for years, exhibiting exceptional resistance to stress and to damages due to the passage of time. Understanding the diapause state could give new insights into strategies to prevent the damage caused by aging and to better preserve organs, tissues, and cells. Thus, the African turquoise killifish brings two interesting aspects to the aging field—a compressed lifespan and a long‐term resistant diapause state, both of which should spark new discoveries in the field.

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