“…For this reason, an early focus in alternative method development was on tests for embryonic malformations (Augustine-Rauch et al, 2010). The most complete reflection of embryonic development apparently can be achieved with zebrafish embryos (Selderslaghs et al, 2011;Sukardi et al, 2011;Weigt et al, 2010Weigt et al, , 2011Yang et al, 2009), for example using dynamic cell imaging, or frog eggs (FETAX assay) (Hoke and Ankley, 2005), which has been evaluated more critically by IC-CVAM 1 . It seems to be timely to evaluate available protocols and datasets and define a protocol for formal validation.…”