Zebrafish type XVII collagen: Gene structures, expression profiles, and morpholino “knock-down” phenotypes

Kim, Seong Hyun; Choi, Hae Young; So, Ju Hoon; Kim, Cheol-Hee; Ho, Shiu Ying; Frank, Michael; Li, Qiaoli; Uitto, Jouni

doi:10.1016/j.matbio.2010.07.002

Cited by 20 publications

(27 citation statements)

References 45 publications

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“…Transcripts encoding likely components of signaling pathways not previously implicated in hair-cell regeneration were also considered of particular interest. Several transcripts previously detected in neuromasts by in situ hybridization, including col17a1b, eya1, sox2, and sox21a, occurred in our narrowed list of mCh + cell-enriched genes, providing evidence that we had successfully captured mantle cells and selected for enriched genes (16,(35)(36)(37).…”

Section: Significancementioning

confidence: 60%

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Dynamic gene expression by putative hair-cell progenitors during regeneration in the zebrafish lateral line

Steiner

Kim

Cabot

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

107

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Hearing loss is most commonly caused by the destruction of mechanosensory hair cells in the ear. This condition is usually permanent: Despite the presence of putative hair-cell progenitors in the cochlea, hair cells are not naturally replenished in adult mammals. Unlike those of the mammalian ear, the progenitor cells of nonmammalian vertebrates can regenerate hair cells throughout life. The basis of this difference remains largely unexplored but may lie in molecular dissimilarities that affect how progenitors respond to hair-cell death. To approach this issue, we analyzed gene expression in hair-cell progenitors of the lateral-line system. We developed a transgenic line of zebrafish that expresses a red fluorescent protein in the presumptive hair-cell progenitors known as mantle cells. Fluorescence-activated cell sorting from the skins of transgenic larvae, followed by microarray-based expression analysis, revealed a constellation of transcripts that are specifically enriched in these cells. Gene expression analysis after hair-cell ablation uncovered a cohort of genes that are differentially regulated early in regeneration, suggesting possible roles in the response of progenitors to hair-cell death. These results provide a resource for studying hair-cell regeneration and the biology of sensory progenitor cells.alkaline phosphatase | auditory | neuromast | supporting cell

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Section: Significancementioning

confidence: 60%

“…The responsiveness of mantle cells to hair-cell death makes them a useful model for identifying genes that control the initiation of regeneration. Only a few molecular markers for these cells have been identified, however, and even fewer have been confirmed as mantle cell-specific (15)(16)(17).…”

mentioning

confidence: 99%

Dynamic gene expression by putative hair-cell progenitors during regeneration in the zebrafish lateral line

Steiner

Kim

Cabot

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

107

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“…Collagen XVII is a heterotrimer of α1 (XVII) chains, each with an intracellular N-terminal domain, a short transmembrane domain and a C-terminal collagenous domain that is cleaved from the cell surface upon the action of proteinases of the ADAMs family (Franzke et al 2005). In addition to the skin, collagen XVII has been shown to be expressed in the buccal mucosa, upper oesophagus, ocular cornea and conjunctiva, bladder, umbilical cord, placenta, retina and neurons of the central nervous system (Van den Bergh and Giudice 2003; Aumailley et al 2006;Huilaja et al 2008;Kim et al 2011). Current data about collagen XVII are mainly derived from studies of the skin and cultured keratinocytes and its exact role in various cell functions, i.e.…”

Section: Introductionmentioning

confidence: 99%

Transmembrane collagen XVII is a novel component of the glomerular filtration barrier

et al. 2012

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The kidney filtration barrier consists of the capillary endothelium, the glomerular basement membrane and the slit diaphragm localized between foot processes of neighbouring podocytes. We report that collagen XVII, a transmembrane molecule known to be required for epithelial adhesion, is expressed in podocytes of normal human and mouse kidneys and in endothelial cells of the glomerular filtration barrier. Immunoelectron microscopy has revealed that collagen XVII is localized in foot processes of podocytes and in the glomerular basement membrane. Its role in kidney has been analysed in knockout mice, which survive to birth but have high neonatal mortality and skin blistering and structural abnormalities in their glomeruli. Morphometric analysis has shown increases in glomerular volume fraction and surface densities of knockout kidneys, indicating an increased glomerular amount in the cortex. Collagen XVII deficiency causes effacement of podocyte foot processes; however, major slit diaphragm disruptions have not been detected. The glomerular basement membrane is split in areas in which glomerular and endothelial basement membranes meet. Differences in the expression of collagen IV, integrins α3 or β1, laminin α5 and nephrin have not been observed in mutant mice compared with controls. We propose that collagen XVII has a function in the attachment of podocyte foot processes to the glomerular basement membrane. It probably contributes to podocyte maturation and might have a role in glomerular filtration.

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“…The latter organ contains 54 neuromasts that are topographically highly conserved neural elements consisting of hair cells, serving as a sensory organ regarding the rheological movements of the fish (Froehlicher et al, 2009). Of interest is our recent finding that the gene product of col17a1b is localized to neuromasts, and “knockdown” of the corresponding gene by a morpholino abolishes the formation of functional neuromasts (Kim et al , 2010). A key difference between the zebrafish and human skin is, however, the lack of mammalian appendages, including hair follicles and sebaceous glands.…”

Section: Biology Of Zebrafish Skin Developmentmentioning

confidence: 99%

“…In many cases, one gene copy of the duplicate genes may have been lost or silenced, but if both copies survive in a functional state, these duplicates in some cases have evolved a novel function in distinct spatial distribution. For example, as indicated below, we have identified two copies of the zebrafish type XVII collagen genes, col17a1a and col17a1b , which by in situ hybridization and morpholino knockdown technologies were shown to have either epidermal or neural distribution, respectively (Kim et al, 2010). In higher vertebrates, including human and mouse, type XVII collagen can display either an epithelial or a neural isoform, which, however, are apparently products of the same gene (Seppänen et al, 2006; Has and Kern, 2010).…”

Section: The Zebrafish Genomementioning

confidence: 99%

Zebrafish: A Model System to Study Heritable Skin Diseases

Frank

Thisse

et al. 2011

Journal of Investigative Dermatology

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Heritable skin diseases represent a broad spectrum of clinical manifestations due to mutations in ~500 different genes. A number of model systems have been developed to advance our understanding of the pathomechanisms of genodermatoses. Zebrafish (Danio rerio), a freshwater vertebrate, has a well-characterized genome, the expression of which can be easily manipulated. The larvae develop rapidly, with all major organs having developed by 5–6 days post fertilization, including the skin, consisting of the epidermis comprising two cell layers and separated from the dermal collagenous matrix by a basement membrane. This perspective highlights the morphologic and ultrastructural features of zebrafish skin, in the context of cutaneous gene expression. These observations suggest that zebrafish provides a useful model system to study molecular aspects of skin development, as well as the pathogenesis and treatment of select heritable skin diseases.

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Zebrafish type XVII collagen: Gene structures, expression profiles, and morpholino “knock-down” phenotypes

Cited by 20 publications

References 45 publications

Dynamic gene expression by putative hair-cell progenitors during regeneration in the zebrafish lateral line

Dynamic gene expression by putative hair-cell progenitors during regeneration in the zebrafish lateral line

Transmembrane collagen XVII is a novel component of the glomerular filtration barrier

Zebrafish: A Model System to Study Heritable Skin Diseases

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