Zebularine enhances apoptosis of human osteosarcoma cells by suppressing methylation of <i><scp>ARHI</scp></i>

Ye, Kaishan; Wang, Shuanke; Wang, Jing; Han, Hua; Ma, Bing; Yong, Yang

doi:10.1111/cas.13088

Cited by 22 publications

(12 citation statements)

References 23 publications

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“…Moreover, miR-10b overexpression and HOXA1 knockdown resulted in aberrant DNMT expression and an increased embryo apoptosis ratio. Previous findings in human cancer cells have shown that cell apoptosis and cell proliferation are related to DNA methylation, and DNA methylation can help inactivate apoptotic pathways at several points (Cho et al, 2011; Ye et al, 2016; Costa et al, 2017; Loginov et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Bta-miR-10b Secreted by Bovine Embryos Negatively Impacts Preimplantation Embryo Quality

et al. 2019

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In a previous study, we found miR-10b to be more abundant in a conditioned culture medium of degenerate embryos compared to that of blastocysts. Here, we show that miR-10b mimics added to the culture medium can be taken up by embryos. This uptake results in an increase in embryonic cell apoptosis and aberrant expression of DNA methyltransferases ( DNMTs ). Using several algorithms, Homeobox A1 ( HOXA1 ) was identified as one of the potential miR-10b target genes and dual-luciferase assay confirmed HOXA1 as a direct target of miR-10b. Microinjection of si- HOXA1 into embryos also resulted in an increase in embryonic cell apoptosis and downregulation of DNMTs . Cell progression analysis using Madin–Darby bovine kidney cells (MDBKs) showed that miR-10b overexpression and HOXA1 knockdown results in suppressed cell cycle progression and decreased cell viability. Overall, this work demonstrates that miR-10b negatively influences embryo quality and might do this through targeting HOXA1 and/or influencing DNA methylation.

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Section: Discussionmentioning

confidence: 99%

Bta-miR-10b Secreted by Bovine Embryos Negatively Impacts Preimplantation Embryo Quality

et al. 2019

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“…DNA demethylation agents and/or histone deacetylation inhibitors can recover DIRAS3 activity in breast and ovarian cancers (Badgwell et al 2011 ). Suppressing methylation of DIRAS3 by Zebularine can elevate DIRAS3 expression and enhances apoptosis in osteosarcoma cells (Ye et al 2016 ). In addition, DNA over-methylation occurs in 79.1% of GC tissue with deficient DIRAS3 expression (Wang et al 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Distinct subgroup of the Ras family member 3 (DIRAS3) expression impairs metastasis and induces autophagy of gastric cancer cells in mice

Qiu

et al. 2018

J Cancer Res Clin Oncol

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PurposeDistinct subgroup of the Ras family member 3 (DIRAS3), also called Aplasia Ras homolog member I, is a tumor suppressor gene that induces autophagy in several cancer cell lines.MethodsThis study analyzed DIRAS3, and markers of autophagy (p62, and LC3B-II) in surgically resected GC samples from 420 patients. The promotion of autophagy by DIRAS3 in gastric cancer (GC) cells was explored, which might explain its inhibitory role in gastric cancer cells.ResultsDIRAS3 expression in GC was positively correlated with LC3B-II amount, and negatively with metastasis; DIRAS3 and p62 levels were independent prognostic factors in GC. Overexpression of DIRAS3 in BGC-823 cells induced autophagy, led to decreased proliferation, cell cycle arrest in G0/G1 phase, increased apoptosis, and impaired migration and invasion. While knockdown of DIRAS3 promoted proliferation and migration in MKN-45 cells. Overexpression of DIRAS3 in BGC-823 cells elevated autophagy levels in subcutaneous xenograft and inhibited tumor growth in mice; the hematogenous liver and lung metastasis of cancer cells were also suppressed.ConclusionsIn conclusion, the results suggest DIRAS3 may play a role in affecting proliferation and metastatic potential of GC cells, which may be associated with its involvement in autophagy regulation.

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“…Previous studies have reported that miR-221 promotes tumor development, while ARHI is a tumor suppressor gene [Chen et al, 2011;Li et al, 2013b;Ye et al, 2015Ye et al, , 2016. We transfected miR-221 mimics and si-ARHI into GAS5-overexpressing MG-63 cells to investigate the role of miR-221 and ARHI on cell proliferation, migration, and EMT of osteosarcoma cells.…”

Section: Gas5 Suppresses Cell Proliferation Migration and Emt Via Regulating Mir-221 And Arhi Expression In Osteosarcoma Cellsmentioning

confidence: 99%

Long Noncoding RNA GAS5 Suppresses Cell Growth and Epithelial–Mesenchymal Transition in Osteosarcoma by Regulating the miR‐221/ARHI Pathway

Wang

Zhang

et al. 2017

J of Cellular Biochemistry

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117

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Dysregulated long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) play key roles in the development of human cancers. The lncRNA growth arrest-specific 5 (GAS5) is reported to be a tumor suppressor in multiple cancers. However, the roles of GAS5 and its related miRNAs in osteosarcoma are poorly understood. This study explored the potential functions and mechanisms of GAS5 in the tumorigenesis of osteosarcoma. Here, the expression of GAS5, miR-221 and aplasia Ras homologue member I (ARHI) was determined in osteosarcoma tissues and cells by Real-time PCR (RT-qPCR). The underlying mechanism of GAS5 in osteosarcoma growth was analyzed via MTT, Transwell, RT-qPCR, Western blot, dual-luciferase reporter assay, RNA immunoprecipitation, and xenograft models after GAS5 overexpression. GAS5 and ARHI levels were significantly reduced, while miR-221 increased, both in osteosarcoma tissues and cells. Overexpression of GAS5 suppressed the proliferation, migration, and epithelial-mesenchymal transition (EMT) of osteosarcoma cells. GAS5 could directly bind to miR-221 to decrease miR-221 expression and enhance ARHI expression. The effect of GAS5 overexpression on the proliferation, migration and EMT was reversed by miR-221 mimics or ARHI siRNA in osteosarcoma cells. Additionally, GAS5 suppressed tumor volume, Ki-67 and PCNA staining, and EMT process in the development of osteosarcoma in vivo. Taken together, lncRNA GAS5 functions as a competing endogenous RNA for miR-221 to suppress cell growth and EMT in osteosarcoma by regulating the miR-221/ARHI pathway. J. Cell. Biochem. 118: 4772-4781, 2017. © 2017 Wiley Periodicals, Inc.

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Zebularine enhances apoptosis of human osteosarcoma cells by suppressing methylation of ARHI

Cited by 22 publications

References 23 publications

Bta-miR-10b Secreted by Bovine Embryos Negatively Impacts Preimplantation Embryo Quality

Bta-miR-10b Secreted by Bovine Embryos Negatively Impacts Preimplantation Embryo Quality

Distinct subgroup of the Ras family member 3 (DIRAS3) expression impairs metastasis and induces autophagy of gastric cancer cells in mice

Long Noncoding RNA GAS5 Suppresses Cell Growth and Epithelial–Mesenchymal Transition in Osteosarcoma by Regulating the miR‐221/ARHI Pathway

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