Zellzählung mittels Einzelobjektelektrochemie

Gooding, J. Justin

doi:10.1002/ange.201606459

Cited by 6 publications

(1 citation statement)

References 16 publications

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“…The observation of these stochastic events can provide information on various single nanoparticles contrary to ensemble (bulk) measurements [12–15] . The main advantage of studying collisions of single entities is the low limit of detection (in principle, one single species) inherent to this electroanalytical method and the ability to study single entities (cells, viruses, nanoparticles, etc.…) in real time (dynamic measurement) [16–27] …”

Section: Figurementioning

confidence: 99%

Detection of Bacterial Rhamnolipid Toxin by Redox Liposome Single Impact Electrochemistry

et al. 2021

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The detection of Rhamnolipid virulence factor produced by Pseudomonas aeruginosa involved in nosocomial infections is reported by using the redox liposome single impact electrochemistry. Redox liposomes based on 1,2dimyristoyl-sn-glycero-3-phosphocholine as a pure phospholipid and potassium ferrocyanide as an encapsulated redox content are designed for using the interaction of the target toxin with the lipid membrane as a sensing strategy. The electrochemical sensing principle is based on the weakening of the liposomes lipid membrane upon interaction with Rhamnolipid toxin which leads upon impact at an ultramicroelectrode to the breakdown of the liposomes and the release/electrolysis of its encapsulated redox probe. We present as a proof of concept the sensitive and fast sensing of a submicromolar concentration of Rhamnolipid which is detected after less than 30 minutes of incubation with the liposomes, by the appearing of current spikes in the chronoamperometry measurement.

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Section: Figurementioning

confidence: 99%

Detection of Bacterial Rhamnolipid Toxin by Redox Liposome Single Impact Electrochemistry

et al. 2021

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Single Fusion Events at Polarized Liquid–Liquid Interfaces

et al. 2016

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An ew electrochemical framework for tracking individual soft particles in solution and monitoring their fusion with polarized liquid-liquid interfaces is reported. The physicochemical principle lies in the interfacial transfer of an ionic probe confined in the particles dispersed in solution and that is released upon their collision and fusion with the fluid interface. As ap roof-of-concept, spike-like transients of as tochastic nature are reported in the current-time response of 1,2dichloroethane(DCE) j water(W) submilli-interfaces after injection of DCE-in-W emulsions.T he sign and potential dependence of the spikes reflect the charge and lipophilicity of the ionic load of the droplets.Acomparison with dynamic light scattering measurements indicates that each spike is associated with the collision of as ingle sub-picoliter droplet. This opens an ew framework for the study of single fusion events at the micro-and nanoscale and of ion transport across biomimetic soft interfaces.

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Detection of Bacterial Rhamnolipid Toxin by Redox Liposome Single Impact Electrochemistry

et al. 2021

View full text Add to dashboard Cite

The detection of Rhamnolipid virulence factor produced by Pseudomonas aeruginosa involved in nosocomial infections is reported by using the redox liposome single impact electrochemistry. Redox liposomes based on 1,2‐dimyristoyl‐sn‐glycero‐3‐phosphocholine as a pure phospholipid and potassium ferrocyanide as an encapsulated redox content are designed for using the interaction of the target toxin with the lipid membrane as a sensing strategy. The electrochemical sensing principle is based on the weakening of the liposomes lipid membrane upon interaction with Rhamnolipid toxin which leads upon impact at an ultramicroelectrode to the breakdown of the liposomes and the release/electrolysis of its encapsulated redox probe. We present as a proof of concept the sensitive and fast sensing of a submicromolar concentration of Rhamnolipid which is detected after less than 30 minutes of incubation with the liposomes, by the appearing of current spikes in the chronoamperometry measurement.

show abstract

Zellzählung mittels Einzelobjektelektrochemie

Abstract: Die Identifikationsnummer (ORCID) des Autors ist unter http://dx.

Cited by 6 publications

References 16 publications

Detection of Bacterial Rhamnolipid Toxin by Redox Liposome Single Impact Electrochemistry

Detection of Bacterial Rhamnolipid Toxin by Redox Liposome Single Impact Electrochemistry

Single Fusion Events at Polarized Liquid–Liquid Interfaces

Detection of Bacterial Rhamnolipid Toxin by Redox Liposome Single Impact Electrochemistry

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