Zeolites as new chromatographic carriers for proteins—easy recovery of proteins adsorbed on zeolites by polyethylene glycol

Chiku, Hiroyuki; Matsui, Masanao; Murakami, Shuntaro; Kiyozumi, Yoshimichi; Mizukami, Fujio; Sakaguchi, Kengo

doi:10.1016/s0003-2697(03)00164-7

Cited by 36 publications

(19 citation statements)

References 14 publications

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“…17 We also found that 5 mg/mL PEG 20000 is effective in refolding various proteins with zeolite. 19 Cleland et al reported that the PEG concentration, as well as its molecular weight, is important in refolding bovine carbonic anhydrase B.…”

Section: Effect Of Peg On Gfp Refoldingmentioning

confidence: 70%

“…In this system, the denatured protein was adsorbed onto zeolite in a 96 DeepWell TM Plate, and the conditions listed in Table 1 were tested. All mixes contained 500 mM Arg and 5 mg/mL PEG 20000 because PEG is required to desorb the proteins from zeolite 17 and Arg is known to enhance refolding by suppressing aggregation. 24,25 The result of the refolding screening of GFP using the 96-well-plate screening system was shown in Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…Zeolites are easy to obtain, stable, and inexpensive compared with other chromatographic carriers. 10 Recently, zeolites have been used in various biochemical applications, such as enzyme immobilization, [11][12][13][14] protein fractionation, [15][16][17] and the selective adsorption of bacteria. 18 We previously reported a simple and effective protein refolding method using a zeolite.…”

Section: Introductionmentioning

confidence: 99%

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High‐throughput protein refolding screening method using zeolite

Nara¹,

Togashi²,

Sekikawa³

et al. 2009

Biotechnology Progress

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We established a 96-well-plate-based refolding screening system using zeolite. In this system, protein denatured and solubilized with 6 M guanidine hydrochloride is adsorbed onto zeolite placed in a 96-well plate. The refolding conditions can be tested by incubating the samples with refolding buffers under various conditions of pH, salts, and additives. In this study, we chose green fluorescent protein as the model protein. Green fluorescent protein was expressed as inclusion bodies, and we tested the effects of four pH conditions and six additives on its refolding. The results demonstrate that green fluorescent protein was more efficiently refolded with zeolite than with the conventional dilution method.

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Section: Effect Of Peg On Gfp Refoldingmentioning

confidence: 70%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High‐throughput protein refolding screening method using zeolite

Nara¹,

Togashi²,

Sekikawa³

et al. 2009

Biotechnology Progress

Self Cite

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“…Zeolite surface interacts with proteins through chelation with Co 2+ on zeolite nanocrystals [268]. The most important advantage of this procedure is lack of the co-concentration of salts during the enrichment process, and the peptides adsorbed on the surface of the zeolite can be directly analyzed by mass spectrometry.…”

Section: Solid-phase Protein Enrichmentmentioning

confidence: 99%

Methods for samples preparation in proteomic research

Bodzoń‐Kułakowska

Bierczyńska-Krzysik

Dyląg

et al. 2007

Journal of Chromatography B

222

199

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“…This low-cost process may lead to production of zeolite NaY to be economically able to compete with established commercial zeolite [16]. Zeolites could selectively adsorb biopolymers like protein, DNA, and RNA, and therefore could be used as chromatographic carriers for these molecules [17][18][19][20]. For these reasons, zeolites would be able to selectively adsorb microbial cells and could be used as cell separation carriers in their native state without surface modifications.…”

Section: Introductionmentioning

confidence: 99%

Novel Synthesis of Nay Zeolite from Rice Husk Silica: Modification with Zno and Zns for Antibacterial Application

Salama¹,

Othman²

2016

Chem Sci J

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Zeolite Y in the sodium form (NaY) was synthesized using amorphous silica ash derived from waste rice husks under hydrothermal conditions. Structural characterization of NaY before and after modification with ZnO and ZnS has been done using powder X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), N 2 adsorption-desorption at -196 °C, scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy. The effects of the Na 2 O/SiO 2 and Si/Al ratios, aging temperature and crystallization time on the optimized synthesis of NaY were thoroughly investigated. Thus the regulated Na + impurity concentration in the starting hydrogel was found to act as a template-assisted synthesis of zeolite NaY by the potential incorporation of Al atoms into the zeolite framework. Results of surface analyses indicate that the interactions of ZnO and ZnS with NaY zeolite are distinguishable and thus the higher interaction is observed for the latter. While Zn II has experienced electrostatic bonding interaction with the framework oxygen atoms, creation of mesopores in NaY due to local destruction of the zeolite lattice around the growing ZnS particles was evidenced. Wonderfully, ZnS/NaY exhibited a high performance in prohibiting the growth of Escherichia coli (E. coli) and negligible from P. Aeruginosa, and these important features make it a potential candidate as an antimicrobial agent for controlling implant-related infections.the utilization of silica from RH for the synthesis of various types of zeolite [8][9][10][11][12][13][14], and mesoporous silica such as . Efforts have also been made to use RH as a source of silica for the synthesis of NaY zeolite with the autoclave process. This low-cost process may lead to production of zeolite NaY to be economically able to compete with established commercial zeolite [16]. Zeolites could selectively adsorb biopolymers like protein, DNA, and RNA, and therefore could be used as chromatographic carriers for these molecules [17][18][19][20]. For these reasons, zeolites would be able to selectively adsorb microbial cells and could be used as cell separation carriers in their native state without surface modifications. Zeolites could adsorb each of the bacterial cell species with high selectivity even from a mixed suspension and could therefore be used as effective carrier materials to provide an easy, rapid and accurate method for cell separation [21]. Zinc oxide supported on zeolite with novel physicochemical properties has recently synthesized [22]. There are some reports regarding the industrial uses of ZnO/ zeolite [23][24][25]. This system can also provide significant impact on the design of a controlled-release product for the pharmacological application in alleviating post-weaning diarrhea of piglets.The aim of this research was to use the RH as an amorphous silica source in the synthesis of NaY zeolite under the optimized experimental conditions. The effects of gel aging and crystallization conditions for the synthesis of NaY under fully optimiz...

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Zeolites as new chromatographic carriers for proteins—easy recovery of proteins adsorbed on zeolites by polyethylene glycol

Cited by 36 publications

References 14 publications

High‐throughput protein refolding screening method using zeolite

High‐throughput protein refolding screening method using zeolite

Methods for samples preparation in proteomic research

Novel Synthesis of Nay Zeolite from Rice Husk Silica: Modification with Zno and Zns for Antibacterial Application

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