Zeptomole Detection of C-Reactive Protein in Serum by a Nanoparticle Amplified Surface Plasmon Resonance Imaging Aptasensor

Vance, Stephen A.; Sandros, Marinella G.

doi:10.1038/srep05129

Cited by 101 publications

(79 citation statements)

References 33 publications

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“…We were able to detect as low as 0.5 ng/mL of gelatin (Figure 7D and Figure S6). 42 The fact that there was almost no change in SPR signal in the common protein solutions but there was over a 100 nm shift in the gelatin solution attests to the NP’s high binding specificity for denatured collagen as well as the potential for sensing collagen denaturation.…”

Section: Resultsmentioning

confidence: 99%

Nanoparticle Assembly and Gelatin Binding Mediated by Triple Helical Collagen Mimetic Peptide

San

Tarbet

et al. 2016

ACS Appl. Mater. Interfaces

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Peptide-conjugated nanoparticles (NPs) have promising potential for applications in biosensing, diagnosis, and therapeutics because of their appropriate size, unique self-assembly, and specific substrate-binding properties. However, controlled assembly and selective target binding are difficult to achieve with simple peptides on NP surfaces because high surface energy makes NPs prone to self-aggregate and adhere nonspecifically. Here, we report the self-assembly and gelatin binding properties of collagen mimetic peptide (CMP) conjugated gold NPs (CMP-NPs). We show that the orientation of CMPs displayed on the NP surface can control NP assembly either by promoting or hindering triple helical folding between CMPs of neighboring NPs. We also show that CMP-NPs can specifically bind to denatured collagen by forming triple-helical hybrids between denatured collagen strands and CMPs, demonstrating their potential use for detection and selective removal of gelatin from protein mixtures. CMP conjugated NPs offer a simple and effective method for NP assembly and for targeting denatured collagens with high specificity. Therefore, they may lead to new types of functional nanomaterials for detection and study of denatured collagen associated with diseases characterized by high levels of collagen degradation.

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Section: Resultsmentioning

confidence: 99%

Nanoparticle Assembly and Gelatin Binding Mediated by Triple Helical Collagen Mimetic Peptide

San

Tarbet

et al. 2016

ACS Appl. Mater. Interfaces

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“…As we previously reported 16,17 , the signal enhancement imparted by the NanoEnhancers is attributed to a mass loading effect and the strong coupling that exists between NIR fluorophores and propagating surface plasmons for gold film nanostructures. Even though, Nano-SPRi adds an extra step to the procedure, this level of sensitivity can widen the applications of SPRi technology in various outlets.…”

Section: Discussionmentioning

confidence: 75%

“…If the concentration of target analyte falls below the SPRi system's limit of detection (<nM), one has to resort to amplifying the SPRi signal via the utilization of nanoparticles (Nano-SPRi). Such SPR-based amplification has been well documented in the literature [16][17][18][19] for various types of analyte and surfaces.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

Vance

Zeidan

Henrich

et al. 2016

JoVE

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Sensitive and selective methods for the detection of human growth hormone (hGH) over a wide range of concentrations (high levels of 50-100 ng ml −1 and minimum levels of 0.03 ng ml −1 ) in circulating blood are essential as variable levels may indicate altered physiology. For example, growth disorders occurring in childhood can be diagnosed by measuring levels of hGH in blood. Also, the misuse of recombinant hGH in sports not only poses an ethical issue it also presents serious health threats to the abuser. One popular strategy for measuring hGH misuse, relies on the detection of the ratio of 22 kDa hGH to total hGH, as non-22 kDa endogenous levels drop after exogenous recombinant hGH (rhGH) administration. Surface plasmon resonance imaging (SPRi) is an analytical tool that allows direct (label-free) monitoring and visualization of biomolecular interactions by recording changes of the refractive index adjacent to the sensor surface in real time. In contrast, the most frequently used colorimetric method, enzyme-linked immunosorbent assay (ELISA) uses enzyme labeled detection antibodies to indirectly measure analyte concentration after the addition of a substrate that induces a color change. To increase detection sensitivity, amplified SPRi uses a sandwich assay format and near infrared quantum dots (QDs) to increase signal strength. After direct SPRi detection of recombinant rhGH in spiked human serum, the SPRi signal is amplified by the sequential injection of detection antibody coated with near-infrared QDs (Nano-SPRi). In this study, the diagnostic potential of direct and amplified SPRi was assessed for measuring rhGH spiked in human serum and compared directly with the capabilities of a commercially available ELISA kit.

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“…More recently, several ultrasensitive SPR biosensors involving QD-conjugated biomolecules have been developed [74][75][76]. In 2014, Sandros' group introduced aptamermodified QDs into an SPRi biosensor and demonstrated a record-high sensitivity of zeptomole (10 −21 moles) detection capability for C-reactive protein [77]. The same group had then shown that such an ultrasensitive platform could operate in multiplexed detection for clinical biomarkers [78].…”

Section: Spr Sensing With Non-plasmonic Nanoparticlesmentioning

confidence: 99%

Recent advances in surface plasmon resonance imaging: detection speed, sensitivity, and portability

et al. 2017

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Surface plasmon resonance (SPR) biosensor is a powerful tool for studying the kinetics of biomolecular interactions because they offer unique real-time and label-free measurement capabilities with high detection sensitivity. In the past two decades, SPR technology has been successfully commercialized and its performance has continuously been improved with lots of engineering efforts. In this review, we describe the recent advances in SPR technologies. The developments of SPR technologies focusing on detection speed, sensitivity, and portability are discussed in details. The incorporation of imaging techniques into SPR sensing is emphasized. In addition, our SPR imaging biosensors based on the scanning of wavelength by a solid-state tunable wavelength filter are highlighted. Finally, significant advances of the vast developments in nanotechnology-associated SPR sensing for sensitivity enhancements are also reviewed. It is hoped that this review will provide some insights for researchers who are interested in SPR sensing, and help them develop SPR sensors with better sensitivity and higher throughput.

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Zeptomole Detection of C-Reactive Protein in Serum by a Nanoparticle Amplified Surface Plasmon Resonance Imaging Aptasensor

Cited by 101 publications

References 33 publications

Nanoparticle Assembly and Gelatin Binding Mediated by Triple Helical Collagen Mimetic Peptide

Nanoparticle Assembly and Gelatin Binding Mediated by Triple Helical Collagen Mimetic Peptide

Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

Recent advances in surface plasmon resonance imaging: detection speed, sensitivity, and portability

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