Zero order and area under curve spectrophotometric methods for determination of Levocetirizine in pharmaceutical formulation

Abstract

Simple, fast and reliable spectrophotometric methods were developed for determination of Levocetirizine in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in methanol. The quantitative determination of the drug was carried out using the zero order derivative values measured at 230 nm and the area under the curve method values measured at 227-234 nm (n=2). Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Levocetirizine using 5-25?g/ml (r=0.998 and r=0.999) for zero order and area under the curve spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. Developed spectrophotometric methods in this study are simple, accurate, precise and sensitive to assay of Levocetirizine in tablets.

Development, Computational Studies and Validation of Spectrophotometric Method of Metformin Hydrochloride in Pharmaceutical Formulations

“…24 Simple ultraviolet (UV) spectrophotometric methods were developed and validated for quantitation of metformin as single or in a binary mixture. [27][28][29][30][31][32][33][34][35][36] The proposed method presents a validated spectrophotometric method for the quantitation of metformin hydrochloride in bulk and pharmaceutical formulations. The method was based on the reaction between metformin and potassium permanganate at room temperature in basic medium.…”

Estimation of the Folic Acid Using Zero Order, Area Under Curve and First Derivative Spectrophotometric Methods in Pure and Marketed Tablet Formulations

“…2. Series of standard solution were prepared using different volumes (3, 5, 7, 9 and 10 ml) from 100 mg.l -1 solution and diluted to 50 ml with distilled water to obtained concretions of pure folic acid equal to (6,10,14,18 and 20 mg.l -1 ). 3.…”

“…For area under curve method (AUC), area was calculated by the computer software program depending on the theoretical equation [16,17,18] : AUC = ∫ A = absorbance of folic acid and ∫ = is area under curve between 296-306 nm.…”