Zerumbone isolated from Zingiber zerumbet inhibits inflammation and pain in rats

Kasolo, Josephine; Bimenya, G. S.; Ojok, Lonzy; Ochieng, Joseph; Ogwal-Okeng, Jasper

doi:10.5897/jmpr10.492

Cited by 101 publications

(33 citation statements)

References 33 publications

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“…Some of the reported metabolites were saponins, tannins, terpenes, steroids, triterpenoids, alkaloids, polyphenolic compounds, flavonoids, carbohydrates, and cardiac glycosides and anthraquinones (20,21). The presence of alkaloids may be responsible for the antimalarial activity of this plant.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of In vivo Antimalarial Activities of leaves of Moringa oleifera against Plasmodium berghei in Mice

Mulisa

Girma

Tesema

et al. 2018

Jundishapur J Nat Pharm Prod

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Background: In addition to resistance against drugs, no new drugs have reached the market during the last decade for malaria treatment. This implies the urgent need to search for new drugs from different sources. Medicinal plants are one of such sources for novel drugs. Therefore, the aim of the current study was to investigate antimalarial activity of crude extract of leaves of Moringa oleifera Lam (Moringaceae) in Plasmodium berghei in a mice model. Methods:The acute toxicity test was used as per standard guidelines. To determine parasite inhibition, 4-day Peter's suppressive test was done. Curative and prophylactic tests were done by applying standard methods. Windows SPSS version 16 was used to analyse the data and analysis of variance (ANOVA) followed by Post Hoc Tukey's HSD to compare results between groups. Results: Death was not observed up to a dose of 2000 mg/kg of the plant extract. In all mice treated with the extract, the level of parasitaemia was significantly reduced in comparison with the control group (P < 0.001) and also a very significant difference was observed in comparison with the chloroquine treated group (P < 0.0001) with lower doses. Mean survival time was significantly increased at all treatment doses. It reduced parasitemia in a dose-dependent manner in both curative and prophylactic activity tests resulting in a significant (P < 0.05) reduction of parasitaemia in comparison with the control group. Conclusions:The result of the current study revealed that the extract possessed significant anti-plasmodial activity as displayed by its ability to suppress P. berghei infection in mice. The present study can be considered as confirmation for traditionally claimed use of the plant against malaria treatment.

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Section: Discussionmentioning

confidence: 99%

Evaluation of In vivo Antimalarial Activities of leaves of Moringa oleifera against Plasmodium berghei in Mice

Mulisa

Girma

Tesema

et al. 2018

Jundishapur J Nat Pharm Prod

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“…The effect of oral administration of AEBO was determined by injection of histamine in the subplantar region of the right hind paw, according to the method described by [11] and [12]. Briefly, rats ( n = 6) were pretreated with AEBO at doses of 50 and 150 mg/kg, for 4 consecutive days, before being injected with 0.1 mL histamine (0.1%).…”

Section: Methodsmentioning

confidence: 99%

Chemical constituents and antihistamine activity of Bixa orellana leaf extract

Yong

Zakaria

Kadir

et al. 2013

BMC Complement Altern Med

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BackgroundBixa orellana L. has been traditionally used in Central and South America to treat a number of ailments, including internal inflammation, and in other tropical countries like Malaysia as treatment for gastric ulcers and stomach discomfort. The current study aimed to determine the major chemical constituents of the aqueous extract of B. orellana (AEBO) and to evaluate the antihistamine activity of AEBO during acute inflammation induced in rats.MethodsAcute inflammation was produced by subplantar injection of 0.1 mL of 0.1% histamine into the right hind paw of each rat in the control and treatment groups. The degree of edema was measured before injection and at the time points of 30, 60, 120, 180, 240 and 300 min after injection. Changes of peritoneal vascular permeability were studied using Evans blue dye as a detector. Vascular permeability was evaluated by the amount of dye leakage into the peritoneal cavity in rats. To evaluate the inhibitory effect of AEBO on biochemical mediators of vascular permeability, the levels of nitric oxide (NO) and vascular endothelial growth factor (VEGF) were determined in histamine-treated paw tissues. The major constituents of AEBO were determined by gas chromatography–mass spectrometry (GC-MS) analysis.ResultsAEBO produced a significant inhibition of histamine-induced paw edema starting at 60 min time point, with maximal percentage of inhibition (60.25%) achieved with a dose of 150 mg/kg of AEBO at 60 min time point. Up to 99% of increased peritoneal vascular permeability produced by histamine was successfully suppressed by AEBO. The expression of biochemical mediators of vascular permeability, NO and VEGF, was also found to be downregulated in the AEBO treated group. Gas chromatography–mass spectrometry (GC-MS) analysis revealed that the major constituent in AEBO was acetic acid.ConclusionsThe experimental findings demonstrated that the anti-inflammatory activity of AEBO was due to its inhibitory effect on vascular permeability, which was suppressed as a result of the reduced expression of biochemical mediators (NO and VEGF) in tissues. Our results contribute towards the validation of the traditional use of Bixa orellana in the treatment of inflammatory disorders.

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“…Its structure possesses three double bonds, two conjugated and one isolated, as well as double conjugated carbonyl group in the 11-membrane ring structure [3] and has been found to possess many biomedical properties such as antiproliferative, antioxidant, antiinflammatory [4][5][6], anticancer [7][8][9][10], and antimicrobial [11][12][13] activities. It possesses antiproliferative properties toward several cancer cell lines mainly on human cervical cancer (HeLa) cell line [14].…”

Section: Introductionmentioning

confidence: 99%

“…Essential oil yielding plants such as Salvia officinales, Syzgium aromaticum, Mentha sps., Eucalyptus sps., and Ocimum basilicum are found to stimulate immune cell proliferation, cytokines expression both in vitro and in vivo [17][18][19]. Zerumbone which is a bioactive compound of essential oil yielding plant of Z. zerumbet have been reported to have antioxidative, anticancer, anti-inflammatory [5,6], and antimicrobial properties [12,13] is yet to be investigated with respect to its immunomodulating activity. Keeping this in view, this study has been designed to find out the immunomodulatory role of zerumbone in modulating the cytokine expression and its effect on the proliferation pattern of lymphocytes in human PBMCs.…”

Section: Introductionmentioning

confidence: 99%

Zerumbone, a Natural Plant Dietary Compound Induces Expression of Interleukin-12p70 Cytokine in Human Peripheral Blood Mononuclear Cells

Dash¹,

Ray²,

Mishra³

et al. 2016

Asian J Pharm Clin Res

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Objective: Despite possessing many biological activities as antiproliferative, antioxidant, anti-inflammatory, and anticancerous, and zerumbone lacks any evidence for its immunomodulatory activity. This naturally occurring dietary compound needs to be developed as drug to support therapeutic claims in various infections and diseases.Methods: Hence, in this study, the immunomodulatory effects of zerumbone were investigated by evaluating the effect of this compound toward the lymphocytes proliferation in human peripheral blood mononuclear cells. Results:Lymphocyte proliferation assay showed that zerumbone was able to activate human lymphocytes at dosage-dependent manner at the highest concentration 40 µl/mL. The production of human interleukin-12p70 cytokine in culture supernatant from activated lymphocytes was upregulated by zerumbone at 24 hrs and gradually decreased at 48 hrs. Hence, the study confirms the immunomodulatory activity of zerumbone which play an important role in boosting up the immune system through cytokine production in dosage dependent manner. Conclusion:The study concludes that zerumbone could be used as a lead molecule in herbal therapeutic world as an immunomodulatory drug in the treatment of chronic infections and various autoimmune disorders.

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Zerumbone isolated from Zingiber zerumbet inhibits inflammation and pain in rats

Cited by 101 publications

References 33 publications

Evaluation of In vivo Antimalarial Activities of leaves of Moringa oleifera against Plasmodium berghei in Mice

Evaluation of In vivo Antimalarial Activities of leaves of Moringa oleifera against Plasmodium berghei in Mice

Chemical constituents and antihistamine activity of Bixa orellana leaf extract

Zerumbone, a Natural Plant Dietary Compound Induces Expression of Interleukin-12p70 Cytokine in Human Peripheral Blood Mononuclear Cells

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