Zerumbone promotes proliferation of endogenous neural stem cells in vascular dementia by regulating Notch signalling

Abstract

Introduction: Present investigation determines the effect of zerumbone on the proliferation of stem cells in vascular dementia (VD) rats. Material and methods: Vascular dementia was induced by cerebral ischemia and reperfusion through non-invasive clamp. Rats were treated with zerumbone 50 mg/kg and 100 mg/kg intraperitoneally 30 min for four weeks after the surgery. Cognitive functions are determined by the Morris water maze (MWM) test and neurological function score in VD rats. Moreover mediators of inflammation and parameters of oxidative stress were estimated in the brain tissue homogenate of ischemia-induced vascular dementia rats. The expression of proteins and mRNA expressions were determined by western blot assay and RT-PCR methods. Moreover histopathological changes were observed by H&E staining on the brain tissue of vascular dementia rats. Results: There was a significant reduction in the cognitive function and neurological score in the zerumbone-treated group compared to the VD group of rats. Data of the study reveal that treatment with zerumbone attenuates the altered level of cytokines and markers of oxidative stress parameters in the brain tissue of VD rats. The expression of NICD, Hes-1 and Nestin proteins was significantly (p < 0.01) reduced in the brain tissue of the zerumbone-treated group compared to the VD group of rats. There was a significant reduction in the mRNA expression of Notch-1 and Hes-1 in the brain tissue of the zerumbone-treated group compared to the VD group of rats. Conclusions: This study concludes that treatment with zerumbone protects the neuronal injury and ameliorates the cognitive function by stimulating the proliferation of endogenous neural stem cells. Moreover proliferation of neural stem cells was stimulated in zerumbone-treated rats by regulating the Notch signalling.

Sophoricoside attenuates neuronal injury and altered cognitive function by regulating the LTR-4/NF-B/PI3K signalling pathway in anaesthetic-exposed neonatal rats

“…Neurological dysfunction was assessed using Longa’s method [ 14 ]. The scoring was as follows: 0, no deficit; 1, forelimb failed to extend entirely and weak; 2, circling to the contralateral side; 3, weight-bearing capacity reduced on the injured side; and 4, no spontaneous locomotor activity.…”

The roles, mechanism, and mobilization strategy of endogenous neural stem cells in brain injury

“…Zerumbone is a sesquiterpenoid and cyclic ketone, which is obtained by steam distillation from Zingiber zerumbet Smith, a type of edible ginger found in southeast Asia particularly ( Pubchem, 2022 ). 50 mg/kg and 100 mg/kg intraperitoneally Zerumbone was reported to stimulate the proliferation of neural stem cells in rats by regulating the Notch signaling ( Sun et al, 2019 ). Osthole was found to promote eNSC proliferation and improve neurological function via the Notch signaling pathway in mice with acute mechanical brain injury ( Yan et al, 2018 ).…”