Zeta Potential Mechanisms Applied to Cellular Immobilization: A Study with Saccharomyces cerevisiae on Dye Adsorption

Mendes, Carolina Rosai; Dilarri, Guilherme; Stradioto, Márcia Regina; Lopes, Paulo Renato Matos; Bidóia, Edério Dino; Montagnolli, Renato Nallin

doi:10.1007/s10924-020-02030-0

Cited by 8 publications

(4 citation statements)

References 33 publications

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“…FT-IR spectra are like molecular fingerprints that provide a valuable insight into chemical structures and their changes due to interactions with other molecules 20 . FT-IR analyses detected the characteristic functional groups associated with the ZnO NPs (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…ZnO NPs were characterized using Fourier transform infrared spectrophotometer FT-IR (Shimadzu Model 8300), adjusted for scanning at 4000–400 cm −1 . For the analysis, a KBr pellet was made with the nanomaterial sample 20 . Micrographs of ZnO NPs were taken by Scanning Electron Microscope (SEM)—JEOL JSM-IT100 operated at 30 kV coupled to a Bruker Quantax Energy Dispersive Detector (EDS), in order to study the morphological characteristics.…”

Section: Methodsmentioning

confidence: 99%

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Antibacterial action and target mechanisms of zinc oxide nanoparticles against bacterial pathogens

Mendes

Dilarri

Forsan

et al. 2022

Sci Rep

Self Cite

282

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Zinc oxide nanoparticles (ZnO NPs) are one of the most widely used nanoparticulate materials due to their antimicrobial properties, but their main mechanism of action (MOA) has not been fully elucidated. This study characterized ZnO NPs by using X-ray diffraction, FT-IR spectroscopy and scanning electron microscopy. Antimicrobial activity of ZnO NPs against the clinically relevant bacteria Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and the Gram-positive model Bacillus subtilis was evaluated by performing resazurin microtiter assay (REMA) after exposure to the ZnO NPs at concentrations ranging from 0.2 to 1.4 mM. Sensitivity was observed at 0.6 mM for the Gram-negative and 1.0 mM for the Gram-positive cells. Fluorescence microscopy was used to examine the interference of ZnO NPs on the membrane and the cell division apparatus of B. subtilis (amy::pspac-ftsZ-gfpmut1) expressing FtsZ-GFP. The results showed that ZnO NPs did not interfere with the assembly of the divisional Z-ring. However, 70% of the cells exhibited damage in the cytoplasmic membrane after 15 min of exposure to the ZnO NPs. Electrostatic forces, production of Zn2+ ions and the generation of reactive oxygen species were described as possible pathways of the bactericidal action of ZnO. Therefore, understanding the bactericidal MOA of ZnO NPs can potentially help in the construction of predictive models to fight bacterial resistance.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Antibacterial action and target mechanisms of zinc oxide nanoparticles against bacterial pathogens

Mendes

Dilarri

Forsan

et al. 2022

Sci Rep

Self Cite

282

View full text Add to dashboard Cite

show abstract

“…ZnO NPs were characterized using Fourier transform infrared spectrophotometer FT-IR (Shimadzu Model 8300), adjusted for scanning at 4000-400 cm −1 . For the analysis, a KBr pellet was made with the nanomaterial sample [19]. Micrographs of ZnO NPs were taken by Scanning Electron Microscope (SEM) -JEOL JSM-IT100 operated at 30 kV coupled to a Bruker Quantax Energy Dispersive Detector (EDS), in order to study the morphological characteristics.…”

Section: Characterization Of the Synthesized Nanomaterialsmentioning

confidence: 99%

“…FT-IR spectra are like molecular ngerprints that provide a valuable insight into chemical structures and their changes due to interactions with other molecules [19]. FT-IR analyses detected the characteristic functional group associated with the ZnO NPs, shown in Figure 3.…”

Section: Equation (1)mentioning

confidence: 99%

Antibacterial Action and Target Mechanisms of Zinc Oxide Nanoparticles Against Bacterial Pathogens

Mendes

Dilarri

Forsan

et al. 2021

Preprint

Self Cite

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Zinc oxide nanoparticles (ZnO NPs) are one of the most widely used nanoparticulate materials due to their antimicrobial properties, but their main mechanism of action (MOA) has not been fully elucidated. The study characterized ZnO NPs using X-ray diffraction, FT-IR spectroscopy and scanning electron microscopy. Antimicrobial activity of clinically bacteria Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa was evaluated by REMA after exposure to the ZnO NP at concentrations from 0.2 to 1.4 mM. Sensitivity was achieved at 0.6 mM for the Gram-negatives and 1.0 mM for Gram-positives cells. The effect of ZnO NPs on the membrane integrity and in the interference of cell division was investigated by its effect on the divisional ring, through fluorescence microscopy assays using B. subtilis (amy::pspac-ftsZ-gfpmut1) expressing FtsZ-GFP. Results showed that ZnO NPs did not interfere with the assembly of the divisional Z-ring. However, 70% of the cells showed damage in the cytoplasmic membrane after 15 min of exposure to the ZnO NPs. Electrostatic forces, production of Zn2+ ions, generation of reactive oxygen species were described as pathways of bactericidal action by ZnO. Thus, understanding bactericidal MOA can produce predictive models to prevent bacterial resistance and lead to further research.

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Anise essential oil immobilized in chitosan microparticles: a novel bactericidal material for food protection

Mendes,

Zortea,

de Souza Laurentino

et al. 2024

Int Microbiol

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Zeta Potential Mechanisms Applied to Cellular Immobilization: A Study with Saccharomyces cerevisiae on Dye Adsorption

Cited by 8 publications

References 33 publications

Antibacterial action and target mechanisms of zinc oxide nanoparticles against bacterial pathogens

Antibacterial action and target mechanisms of zinc oxide nanoparticles against bacterial pathogens

Antibacterial Action and Target Mechanisms of Zinc Oxide Nanoparticles Against Bacterial Pathogens

Anise essential oil immobilized in chitosan microparticles: a novel bactericidal material for food protection

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