ZFP57 regulates DNA methylation of imprinted genes to facilitate embryonic development of somatic cell nuclear transfer embryos in Holstein cows

Yu, Tong; Meng, Ru; Song, Weijia; Sun, Hongzheng; An, Quanli; Zhang, Chengtu; Zhang, Yong; Su, Jianmin

doi:10.3168/jds.2022-22427

Cited by 5 publications

(2 citation statements)

References 44 publications

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“…Such a situation leads to disturbances in the energy metabolism in oocytes and embryos, which fundamentally impacts their developmental competence. Finally, precisely recognizing the biological factors responsible for the heat stress-mediated induction of impairments in reproductive physiology and endocrinology in cattle and other mammalian species might enable the multifaceted estimation of genomic, epigenomic, transcriptomic and proteomic profiles in the embryos, fetuses and offspring created and multiplied by such novel assisted reproductive technologies and in vitro embryo production strategies as the ex vivo fertilization of oocytes, with the aid of either classic penetration by capacitated spermatozoa [ 91 , 92 ] or intra-cytoplasmic sperm injection [ 93 , 94 ] and somatic cell cloning [ 95 , 96 ]. The harmful effects of heat stress on cow reproduction are of key importance to the economic status of the farm, producing financial losses every year.…”

Section: Discussionmentioning

confidence: 99%

Effects of Heat Stress on Bovine Oocytes and Early Embryonic Development—An Update

Miętkiewska

Kordowitzki

Pareek

2022

Cells

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Heat stress is a major threat to cattle reproduction today. It has been shown that the effect of high temperature not only has a negative effect on the hormonal balance, but also directly affects the quality of oocytes, disrupting the function of mitochondria, fragmenting their DNA and changing their maternal transcription. Studies suggest that the induction of HSP70 may reduce the apoptosis of granular layer cells caused by heat stress. It has been shown that the changes at the transcriptome level caused by heat stress are consistent with 46.4% of blastocyst development disorders. Cows from calves exposed to thermal stress in utero have a lower milk yield in their lifetime, exhibit immunological disorders, have a lower birth weight and display a shorter lifespan related to the expedited aging. In order to protect cow reproduction, the effects of heat stress at the intracellular and molecular levels should be tracked step by step, and the impacts of the dysregulation of thermal homeostasis (i.e., hyperthermy) should be taken into account.

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Section: Discussionmentioning

confidence: 99%

Effects of Heat Stress on Bovine Oocytes and Early Embryonic Development—An Update

Miętkiewska

Kordowitzki

Pareek

2022

Cells

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“…ZFP57 is a key protein needed for imprinting maintenance after fertilization, and its expression can maintain normal methylation in the imprinting control region. Yu et al found that the methylation level of the imprinting control region of key imprinted genes in SCNT-cloned embryos was lower than that of in vitro fertilization embryos, indicating the loss of imprinted gene methylation [123]. Increasing the expression of ZFP57 in donor cells promoted the development of SCNT blastocysts, increased the number of trophoblast cells and total cells, decreased the rate of apoptosis and achieved a degree of methylation similar to that of in vitro fertilized embryos, which provided an effective means to enhance the nuclear reprogramming and developmental potential of SCNT embryos.…”

Section: Studies On the Relationship Between Dna Methylation And Bovi...mentioning

confidence: 99%

Effects of DNA Methylation on Gene Expression and Phenotypic Traits in Cattle: A Review

Zhang

Sheng

et al. 2023

IJMS

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Gene expression in cells is determined by the epigenetic state of chromatin. Therefore, the study of epigenetic changes is very important to understand the regulatory mechanism of genes at the molecular, cellular, tissue and organ levels. DNA methylation is one of the most studied epigenetic modifications, which plays an important role in maintaining genome stability and ensuring normal growth and development. Studies have shown that methylation levels in bovine primordial germ cells, the rearrangement of methylation during embryonic development and abnormal methylation during placental development are all closely related to their reproductive processes. In addition, the application of bovine male sterility and assisted reproductive technology is also related to DNA methylation. This review introduces the principle, development of detection methods and application conditions of DNA methylation, with emphasis on the relationship between DNA methylation dynamics and bovine spermatogenesis, embryonic development, disease resistance and muscle and fat development, in order to provide theoretical basis for the application of DNA methylation in cattle breeding in the future.

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Single-cell RNA-seq and single-cell bisulfite-sequencing reveal insights into yak preimplantation embryogenesis

Yu,

Zhang,

Song

et al. 2024

Journal of Biological Chemistry

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ZFP57 regulates DNA methylation of imprinted genes to facilitate embryonic development of somatic cell nuclear transfer embryos in Holstein cows

Cited by 5 publications

References 44 publications

Effects of Heat Stress on Bovine Oocytes and Early Embryonic Development—An Update

Effects of Heat Stress on Bovine Oocytes and Early Embryonic Development—An Update

Effects of DNA Methylation on Gene Expression and Phenotypic Traits in Cattle: A Review

Single-cell RNA-seq and single-cell bisulfite-sequencing reveal insights into yak preimplantation embryogenesis

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