Zhihengliuella aestuarii sp. nov., isolated from tidal flat sediment

Abstract

A Gram-stain-positive, ovoid to short rod-shaped, yellow-pigmented bacterium, designated strain DY66T , was isolated from tidal-flat sediment collected from Deukryang Bay (Republic of Korea), and its taxonomic position was investigated by using a polyphasic approach. Strain DY66 T grew optimally at 30 6C and pH 8-9 and in 2 % (w/v) NaCl. The peptidoglycan type was A4a, L-Lys-L-Ala-D-Glu, and tyvelose and glucose were the major cell-wall sugars. The predominant menaquinones were MK-10 and MK-9. Major cellular fatty acids (.10 % of total) were anteiso-C 15 : 0 and iso-C 15 : 0 . The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, an unknown aminophospholipid and three unknown aminolipids. The DNA G+C content was 59.1 mol%. This chemotaxonomic profile supported the assignment of strain DY66 T to the genus Zhihengliuella. Phylogenetic analysis based on 16S rRNA gene sequences also indicated that strain DY66 T belonged to the family Micrococcaceae and was related to the genus Zhihengliuella. On the basis of the evidence presented in this study, strain DY66

Zhihengliuella somnathii sp. nov., a halotolerant actinobacterium from the rhizosphere of a halophyte Salicornia brachiata

“…DNA–DNA hybridization was carried out as described by De Ley et al (1970) under consideration of the modifications described by Huss et al (1983). The G+C content of strains JG 03 T and JG 05 was 70.0 mol% and 70.1 mol%, respectively, which are similar to the values (59.1–70.3 %) reported for other species of the genus Zhihengliuella (Zhang et al , 2007; Tang et al , 2009; Chen et al , 2010; Baik et al , 2011; Hamada et al , 2013). DNA–DNA hybridization of JG 03 T with Z. alba DSM 21143 T , Z. halotolerans DSM 17364 T , Z. salsuginis DSM 21149 T , Z. flava DSM 26152 T and Z. aestuarii KCTC 19557 T showed reassociation values of 53.4 %, 49.6 %, 37.2 %, 25.9 % and 19.8 %, respectively.…”

“…The genus Zhihengliuella was proposed by Zhang et al (2007). At the time of writing, the genus Zhihengliuella contains five species with validly published names: Zhihengliuella halotolerans (Zhang et al , 2007), Zhihengliuella alba (Tang et al , 2009), Zhihengliuella salsuginis (Chen et al , 2010), Zhihengliuella aestuarii (Baik et al , 2011) and Zhihengliuella flava (Hamada et al , 2013).…”

“…The major component of the cell-wall sugar was galactose with minor amounts of glucose, mannose and rhamnose also detected in both strains (JG 03 T and JG 05). Glucose and tyvelose were the major cell-wall sugars in Z. halotolerans and Z. aestuarii (Zhang et al, 2007; Baik et al , 2011), whereas mannose and tyvelose were those in Z. alba and Z. salsuginis (Tang et al , 2009; Chen et al, 2010). In the case of Z. flava, however, galactose was also present as a major constituent (Hamada et al , 2013).…”

“… * Data from Baik et al (2011). † Data from Tang et al (2009). ‡ Data from Chen et al (2010). § Data from Zhang et al (2007). ‖ Data from Hamada et al (2013). …”

Salt tolerance of Cressa cretica and its rhizosphere microbiota

“…In the present study, various halotolerant bacteria were recorded in the soil and rhizosphere samples such as Inmirania sp., a Gamma-Proteobacterium known for halophilic representatives (e.g. the thermophilic, facultative autotrophic, sulfur-oxidizing I. thermothiophila; Slobodkina et al 2016) as well as Zhihengiuella, a halophilic genus found in tidal flats (Baik et al 2011). In addition, most of the other genera contributing to the differentiation of the rhizosphere and bulk soils are also known from other saline habitats.…”