Zika Virus as an Emerging Global Pathogen

Beckham, J. David; Pastula, Daniel M.; Massey, Aaron; Tyler, Kenneth L.

doi:10.1001/jamaneurol.2016.0800

Cited by 68 publications

(28 citation statements)

References 42 publications

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“…In humans, congenital microcephaly and additional birth defects result in part from infection of the fetal neuronal stem cells 40,41 . Our recent research showed that ZIKV can also infect adult mouse neural progenitor cells, resulting in reduced cell proliferation and cell death 39 .…”

Section: Discussionmentioning

confidence: 99%

Identification of Zika virus epitopes reveals immunodominant and protective roles for dengue virus cross-reactive CD8+ T cells

et al. 2017

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CD8+ T cells play an important role in controlling Flavivirus infection, including Zika virus (ZIKV). Here, we have identified 25 HLA-B*0702-restricted epitopes and 1 HLA-A*0101-restricted epitope using interferon (IFN)-γ enzyme-linked immunospot (ELISPOT) and intracellular cytokine staining (ICS) in ZIKV-infected IFN-α/β receptor-deficient HLA transgenic mice. The cross-reactivity of ZIKV epitopes to dengue virus (DENV) was tested using IFN-γ-ELISPOT and IFN-γ-ICS on CD8+ T cells from DENV-infected mice, and five cross-reactive HLA-B*0702-binding peptides were identified by both assays. ZIKV/DENV cross-reactive CD8+ T cells in DENV-immune mice expanded post ZIKV challenge and dominated in the subsequent CD8+ T cell response. ZIKV challenge following immunization of mice with ZIKV-specific and ZIKV/DENV cross-reactive epitopes elicited CD8+ T cell responses that reduced infectious ZIKV levels, and CD8+ T cell depletions confirmed that CD8+ T cells mediated this protection. These results identify ZIKV-specific and ZIKV/DENV cross-reactive epitopes and demonstrate both an altered immunodominance pattern in the DENV-immune setting relative to naive, as well as a protective role for epitope-specific CD8+ T cells against ZIKV. These results have important implications for ZIKV vaccine development and provide a mouse model for evaluating anti-ZIKV CD8+ T cell responses of human relevance.

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Section: Discussionmentioning

confidence: 99%

Identification of Zika virus epitopes reveals immunodominant and protective roles for dengue virus cross-reactive CD8+ T cells

et al. 2017

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“…Here, we used PPMC with finger electrodes to detect ZIKV through electrical sensing of viral lysate. ZIKV is a newly emerging flavivirus that has been of major international public health concern following large outbreaks in the Americas . The increasing number of studies that confirm the linkage between ZIKV and birth defects calls for the urgent need for the development of low‐cost, simple, and rapid POC tests.…”

Section: Resultsmentioning

confidence: 99%

Hybrid Paper–Plastic Microchip for Flexible and High‐Performance Point‐of‐Care Diagnostics

Draz

Moazeni

Venkataramani

et al. 2018

Adv Funct Materials

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A low-cost and easy-to-fabricate microchip remains a key challenge for the development of true point-of-care (POC) diagnostics. Cellulose paper and plastic are thin, light, flexible, and abundant raw materials, which make them excellent substrates for mass production of POC devices. Herein, a hybrid paper–plastic microchip (PPMC) is developed, which can be used for both single and multiplexed detection of different targets, providing flexibility in the design and fabrication of the microchip. The developed PPMC with printed electronics is evaluated for sensitive and reliable detection of a broad range of targets, such as liver and colon cancer protein biomarkers, intact Zika virus, and human papillomavirus nucleic acid amplicons. The presented approach allows a highly specific detection of the tested targets with detection limits as low as 102 ng mL−1 for protein biomarkers, 103 particle per milliliter for virus particles, and 102 copies per microliter for a target nucleic acid. This approach can potentially be considered for the development of inexpensive and stable POC microchip diagnostics and is suitable for the detection of a wide range of microbial infections and cancer biomarkers.

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“…Given that TLR-3 is also specifically elevated in ZIKV infection (Hamel et al 2015;Dang et al 2016), it is again plausible that weakening of the BBB could lead to CNS infection in adult subjects. ZIKV outbreaks in French Polynesia and Brazil have both been associated with increased cases of paralytic neuropathy characteristic of GBS (Beckham et al 2016;Lucchese and Kanduc 2016;Brasil et al 2016). A case-control analysis of the French Polynesia cases showed a clear association between patients with GBS and a recent infection, as well as neutralizing antibodies against ZIKV (Cao-Lormeau et al 2016).…”

Section: Zikv Infection and The Risk Of Neurological Diseasesmentioning

confidence: 98%

Zika virus as a causative agent for primary microencephaly: the evidence so far

Tang

2016

Arch Microbiol

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Zika virus (ZIKV) infection has been associated with congenital microcephaly and peripheral neuropathy. The ongoing epidemic has triggered swift responses in the scientific community, and a number of recent reports have now confirmed a causal relationship between ZIKV infection and birth defect. In particular, ZIKV has been shown to be capable of compromising and crossing the placental barrier and infect the developing fetal brain, resulting in the demise and functional impairment of neuroprogenitor cells critical for fetal cortex development. Here, the evidence for ZIKV as a teratogenic agent that causes microcephaly is reviewed, and its association with other disorders is discussed.

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Zika Virus as an Emerging Global Pathogen

Cited by 68 publications

References 42 publications

Identification of Zika virus epitopes reveals immunodominant and protective roles for dengue virus cross-reactive CD8+ T cells

Identification of Zika virus epitopes reveals immunodominant and protective roles for dengue virus cross-reactive CD8+ T cells

Hybrid Paper–Plastic Microchip for Flexible and High‐Performance Point‐of‐Care Diagnostics

Zika virus as a causative agent for primary microencephaly: the evidence so far

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