Zinc, a Component of Yeast Alcohol Dehydrogenase

Vallée, Bert L.; Hoch, Frederic L.

doi:10.1073/pnas.41.6.327

Cited by 358 publications

(85 citation statements)

References 10 publications

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“…Cytosolic contamination of nuclei was assayed by determining the activity of alcohol dehydrogenase (25) in the homogenate, cytosol, and the washed nuclei preparation and was found to be <0.5% in the nuclear preparation. The average recovery of DNA in the nuclear preparation was 72.8% of that in the homogenate.…”

Section: Estrogen Binding Studiesmentioning

confidence: 99%

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Sex Hormone-Related Functions in Regenerating Male Rat Liver

Francavilla¹,

Eagon²,

DiLeo³

et al. 1986

Gastroenterology

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Sex hormone receptors were quantitated in normal male rat liver and in regenerating liver at several different times after partial (70%) hepatectomy. Both estrogen and androgen receptor content were altered dramatically by partial hepatectomy. Total hepatic content and nuclear retention of estrogen receptors increased, with the zenith evident 2 days after partial hepatectomy, corresponding to the zenith of mitotic index. Serum estradiol increased after 1 day, and reached a maximum at 3 days after surgery. In contrast, total and nuclear androgen receptor content demonstrated a massive decline at 1, 2, and 3 days after resection. Serum testosterone displayed a parallel decline. In addition, hepatic content of two androgen-responsive proteins was reduced to 15% and 13% of normal values during this period. The activity of these various proteins during regeneration of male rat liver is comparable to that observed in the liver of normal female rats. Taken together, these results indicate that partial hepatectomy induces a feminization of certain sexually dimorphic aspects of liver function in male rats. Furthermore, these data provide evidence that estrogens, but not androgens, may have an important role in the process of liver regeneration.Mammalian liver of both sexes is responsive to sex hormones. Liver is estrogen responsive in that it contains receptors for this hormone and responds by the synthesis of specific proteins (reviewed in Reference 1). Liver contains androgen receptors as well (2-5). In fact, the sexually dimorphic hepatic content of certain receptors and microsomal enzymes observed in male rats is maintained by testosterone (reviewed in References 1 and 6). Ultimate control over these sex differences in hepatic function resides in the pituitary gland. In particular, growth hormone secretion patterns, also sexually dimorphic in nature, appear to regulate hepatic levels of many microsomal steroid and drug metabolizing enzymes as well as steroid receptor levels (1,7,8).Since Higgins and Anderson originally reported (9) the technique of partial hepatectomy (PH) in rats, investigators have been studying the effect of hepatic resection and the remnant liver with the goal of identifying the mechanisms that regulate hepatic regeneration. During the last 10 yr, hormones have assumed a prominent role as factors related in some way to hepatic regeneration after PH. It has been suggested that hormonal agents such as insulin, glucagon, and epidermal growth factor may initiate the process of regeneration (10-17); however, other hormones such as parathyroid hormone (18) (23). These data demonstrated that the proliferative activity of the regenerating liver is concurrent with a significant increase in nuclear localization of hepatic ERs. However, this study did not examine possible increases in serum estradiol content that might account for the increased nuclear ER localization, nor was total hepatic content of ER examined. We have now extended the study to answer these questions, and in addition, to determin...

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Section: Estrogen Binding Studiesmentioning

confidence: 99%

“…Nuclei prepared this way appeared rounded under light microscopy and stained blue with hematoxylin and eosin. The final nuclear preparation contained no detectable cytosolic contamination as judged by lack of specific cytosolic staining (above) and by assay for alcohol dehydrogenase activity (25).…”

Section: Androgen Binding Studiesmentioning

confidence: 99%

Sex Hormone-Related Functions in Regenerating Male Rat Liver

Francavilla¹,

Eagon²,

DiLeo³

et al. 1986

Gastroenterology

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“…Since there is only one zinc atom per subunit of YADH (17), it would appear that the "structural" (35,36) zinc atom is missing. Its function in the dimeric LADH may, presumably, therefore be replaced by the tetrameric structure of YADH.…”

Section: Structure Of Yadh Compared With Other Dehydrogenasesmentioning

confidence: 99%

“…Thus, both YADH and LADH have alcohol as substrate and contain zinc (17,18), in contrast to LDH (13) and GPDH (19). On the other hand, YADH, LDH, and GPDH are all tetrameric with subunits of molecular weight about 36,000 (7,13,20), whereas LADH is dimeric with subunits of molecular weight 40,000 (21).…”

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confidence: 99%

Partial Similarities Between Yeast and Liver Alcohol Dehydrogenases

Jörnvall

1973

Proc. Natl. Acad. Sci. U.S.A.

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The primary structure of about half of the protein chain of yeast alcohol dehydrogenase has been determined and compared with the amino-acid sequences of other dehydrogenases. The enzyme is found to be distantly related to horse-liver alcohol dehydrogenase, although these two proteins have different quaternary structures and subunit sizes. Some regions show no significant similarities, but long segments within the N-terminal parts of the molecules are homologous, suggesting a common and important function for these segments. Ancestral connections between some different dehydrogenases can be concluded and the degree of evolutionary changes may be estimated.

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“…It is noteworthy that zinc is found in the structure of superoxide dismutase, the enzyme involved in oxy-redox reactions [24]. Zinc is a functional component of alcohol dehydrogenase [25]. It stabilises the structure of zinc fingers, plays an important role in DNA transcription and repair, division and apoptosis of cells.…”

Section: Discussionmentioning

confidence: 99%

Relationships between serum selenium and zinc concentrations versus profibrotic and proangiogenic cytokines (FGF-19 and endoglin) in patients with alcoholic liver cirrhosis

Prystupa¹,

Kiciński²,

Luchowska-Kocot³

et al. 2017

Ann Agric Environ Med.

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Relationships between serum selenium and zinc concentrations versus profibrotic and proangiogenic cytokines in patients with alcoholic liver cirrhosis. Ann Agric Env Med. 2017; 24(3): 544-548. doi: 10.26444/aaem/76937 Abstract Introduction and objective. Liver cirrhosis is a disease involving the liver parenchyma, which is characterised by fibrosis. and impaired architectonics of the parenchyma with regenerative nodules. The aim of the study was to determine the relationship between stage of alcoholic liver cirrhosis, concentrations of selenium, zinc and profibrotic and proangiogenic cytokines ENG). Materials and method. The study included 99 patients with alcoholic cirrhosis and 20 healthy subjects. Ion chromatography with UV/VIS detection was used for determination of zinc ions in the previously mineralized serum samples. The measurements of selenium were performed with the ContrAA700 high-resolution continuum source graphite tube atomic absorption spectrometer. ELISA was used to determine concentration of FGF-19 and ENG in serum samples. Results. Concentrations of zinc and selenium were significantly decreased in cirrhotic patients (p<0.001 for both). The highest concentration of FGF-19 was found in Child-Pugh stage C liver cirrhosis patients (806.9±650.3 pg/ml), and was significantly higher than observed in controls (p=0.005) and stage A patients (compensated cirrhosis) (p=0.02). The highest concentration of ENG was demonstrated in the control group (3.24±148 ng/ml) while the lowest in patients with decompensated cirrhosis (7.32±5.39 ng/ml and 7.92±4.18 ng/ml for stage B and C; p=0.03 and p=0.02, respectively). The use of the multiple-variable model demonstrated that the independent factors affecting the concentration of ENG were the concentration of bilirubin (p=0.02), INR (p=0.01) and duration of alcohol abuse (p=0.02). The independent determinants of FGF-19 concentrations were found to be the stage (severity) of liver cirrhosis (p=0.04) and INR (p=0.03). Conclusions. Concentrations of zinc and selenium in serum of patients with alcoholic liver cirrhosis are not independently related to concentrations of FGF-19 and ENG.

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Zinc, a Component of Yeast Alcohol Dehydrogenase

Cited by 358 publications

References 10 publications

Sex Hormone-Related Functions in Regenerating Male Rat Liver

Sex Hormone-Related Functions in Regenerating Male Rat Liver

Partial Similarities Between Yeast and Liver Alcohol Dehydrogenases

Relationships between serum selenium and zinc concentrations versus profibrotic and proangiogenic cytokines (FGF-19 and endoglin) in patients with alcoholic liver cirrhosis

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