Zinc-cadmium interactions instigated antagonistic alterations in lipid peroxidation, ascorbate peroxidase activity and chlorophyll synthesis in Phaseolus vulgaris leaves

Apiamu, Augustine; Asagba, Samuel Ogheneovo

doi:10.1016/j.sciaf.2020.e00688

Cited by 9 publications

(3 citation statements)

References 37 publications

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“…The suitability of these models was further validated by high Fisher's F test and low p value obtained in this study, as an indication of significance reported by Anuar et al [44]. In The use of lack of fit tests was also reported as criteria of acceptability and adequacy of a model with an indication of non significant effect (p > 0.05), which implies the best fitting of experimental data, as required [41,45,46]. The quadratic models for the prediction of NKA activity in all tissues showed a lack of fit with comparable critical F and p values, and this suggested significant goodness of fit for the experimental data.…”

Section: Discussionsupporting

confidence: 66%

“…However, there may be alterations of its functionality when biological systems are exposed to toxicants in their environment, as observed in this study. It is a priority for environmental toxicologists to characterize the effects of toxicants in various dimensions on biological systems using different research methodologies [3,4,17,41]. BBD in this study was chosen due to its robustness to identify and understand the nature of the interaction between the toxicants, (specifically Pb and Zn), on exposed African catfish through the biomonitoring of NKA activity, and comparing the predicted responses in three different fish tissues with observed responses using response surface models.…”

Section: Discussionmentioning

confidence: 99%

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Exposure of African Catfish (Clarias gariepinus) to Lead and Zinc Modulates Membrane-Bound Transport Protein: A Plausible Effect on Na+/K+-ATPase Activity

Apiamu

Osawaru

Asagba

et al. 2021

Biol Trace Elem Res

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The contamination of the aquatic ecosystem beyond tolerable limits may pose serious health challenges to its components. This study evaluated the toxic effects of a binary mixture of lead (Pb) and zinc (Zn) compounds on the activity of Na + /K + -ATPase in tissues of Clarias gariepinus in a controlled aquatic system. The study employed Box-Behnken Design (BBD) with 17 runs in which Pb and Zn concentrations were considered process variables in a time-dependent fashion. Metal exposure levels consisted of 0, 10 and 20% of 96 h-LC 50 of Pb (55.12 mg/L) and Zn (32.15 mg/L) for three weeks. Thereafter, membrane-bound Na + /K + -ATPase activity was assessed in gill, hepatic and renal tissues, and data generated from the BBD were used for the development of models. Three regression models were obtained, for gill, hepatic and renal Na + /K + -ATPase activities with exposure to metals differ significantly (p < 0.05) at R 2 > 90%, and no significant lack of fit (p > 0.05) was observed in each case. Congruent to the synergistic interactions observed between Pb and Zn in the study, the gill and hepatic Na + /K + -ATPase activities were significantly inhibited, whereas renal Na + /K + -ATPase activity was significantly stimulated (p < 0.05). The optimized models were considered reliable, as they were confirmed in the laboratory through accurate prediction of hepatic, renal and gill Na + /K + -ATPase activities with equivalences of 1.22 ± 0.17, 1.66 ± 0.07 and 3.50 ± 0.33 µmol p i /min/mg protein (p < 0.05) respectively. It is hereby concluded that the synergistic interaction between Pb and Zn truncated the physiological function of Na +/ K + -ATPase activity in the respective tissues except for renal tissue of exposed C. gariepinus. Keywords C. gariepinus • Lead • Na + /K + -ATPase activity • Toxic effects • Zinc * Augustine Apiamu

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Exposure of African Catfish (Clarias gariepinus) to Lead and Zinc Modulates Membrane-Bound Transport Protein: A Plausible Effect on Na+/K+-ATPase Activity

Apiamu

Osawaru

Asagba

et al. 2021

Biol Trace Elem Res

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“…The activity of ascorbate peroxidase (APX) was determined by referring to Apiamu et al [50]. The reaction mixture included enzyme extract (100 µL), 7.5 mM ascorbate (100 µL), 0.05 M phosphate buffer (2700 µL) and hydrogen peroxide (100 µL).…”

Section: Antioxidant Enzyme Activitymentioning

confidence: 99%

Effect of Heat Treatment on the Quality and Soft Rot Resistance of Sweet Potato during Long-Term Storage

Wu,

Zhang,

et al. 2023

Foods

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Heat treatment is a widely applied technique in the preservation of fruits and vegetables, effectively addressing issues such as disease management, rot prevention, and browning. In this study, we investigated the impact of heat treatment at 35 °C for 24 h on the quality characteristics and disease resistance of two sweet potato varieties, P32/P (Ipomoea batatas (L.) Lam. cv ‘Pushu13’) and Xinxiang (Ipomoea batatas (L.) Lam. cv ‘Xinxiang’). The growth in vitro and reproduction of Rhizopus stolonifer were significantly inhibited at 35 °C. However, it resumed when returned to suitable growth conditions. The heat treatment (at 35 °C for 24 h) was found to mitigate nutrient loss during storage while enhancing the structural characteristics and free radical scavenging capacity of sweet potato. Additionally, it led to increased enzyme activities for APX, PPO, and POD, alongside decreased activities for Cx and PG, thereby enhancing the disease resistance of sweet potato against soft rot. As a result, the heat treatment provided a theoretical basis for the prevention of sweet potato soft rot and had guiding significance for improving the resistance against sweet potato soft rot.

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Responses of riparian plant roots to acute combined nitrogen and phosphorus stress: changes in root morphology and antioxidant system

et al. 2023

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Zinc-cadmium interactions instigated antagonistic alterations in lipid peroxidation, ascorbate peroxidase activity and chlorophyll synthesis in Phaseolus vulgaris leaves

Cited by 9 publications

References 37 publications

Exposure of African Catfish (Clarias gariepinus) to Lead and Zinc Modulates Membrane-Bound Transport Protein: A Plausible Effect on Na+/K+-ATPase Activity

Exposure of African Catfish (Clarias gariepinus) to Lead and Zinc Modulates Membrane-Bound Transport Protein: A Plausible Effect on Na+/K+-ATPase Activity

Effect of Heat Treatment on the Quality and Soft Rot Resistance of Sweet Potato during Long-Term Storage

Responses of riparian plant roots to acute combined nitrogen and phosphorus stress: changes in root morphology and antioxidant system

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