Zinc-doped dentin adhesive for collagen protection at the hybrid layer

Osorio, Raquel; Yamauti, Mónica; Osorio, Estrella; Román, Julio San; Toledano, Manuel

doi:10.1111/j.1600-0722.2011.00853.x

Cited by 62 publications

(85 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Zinc has been demonstrated to reduce MMPs-mediated collagen degradation (Osorio et al, 2011), to inhibit dentin demineralization (Takatsuka et al, 2005) and to induce dentin remineralization at the bonded interface (Toledano et al, 2013a). Zinc influences signaling pathways and stimulates a metabolic effect in hard tissue mineralization (Hoppe et al, 2011) and remineralization processes (Barcellos et al, 2016;Lynch et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Toledano

Osorio

et al. 2017

Journal of the Mechanical Behavior of Biomedical Materials

Self Cite

View full text Add to dashboard Cite

The aim of this study was to evaluate changes in the mechanical and chemical behavior, and bonding ability at dentin interfaces infiltrated with polymeric nanoparticles (NPs) prior to resin application. Dentin surfaces were treated with 37% phosphoric acid followed by application of an ethanol suspension of NPs, Zn-NPs or Ca-NPs followed by the application of an adhesive, Single Bond (SB). Bonded interfaces were stored for 24 h, submitted to microtensile bond strength test, and evaluated by scanning electron microscopy. After 24 h and 21 d of storage, the whole resin-dentin interface adhesive was evaluated using a Nano-DMA. Complex modulus, storage modulus and tan delta (δ) were assessed. AFM imaging and Raman analysis were performed. Bond strength was not affected by NPs infiltration. After 21 d of storage, tan δ generally decreased at Zn-NPs/resin-dentin interface, and augmented when Ca-NPs or non-doped NPs were used. When both Zn-NPs and Ca-NPs were employed, the storage modulus and complex modulus decreased, though both moduli increased at the adhesive and at peritubular dentin after Zn-NPs infiltration. The phosphate and the carbonate peaks, and carbonate substitution, augmented more at interfaces promoted with Ca-NPs than with Zn-NPs after 21 d of storage, but crystallinity did not differ at created interfaces with both ions-doped NPs. Crosslinking of collagen and the secondary structure of collagen improved with Zn-NPs resin-dentin infiltration. Ca-NPs-resin dentin infiltration produced a favorable dissipation of energy with minimal stress concentration trough the crystalline remineralized resin-dentin interface, causing minor damage at this structure.

show abstract

Section: Introductionmentioning

confidence: 99%

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Toledano

Osorio

et al. 2017

Journal of the Mechanical Behavior of Biomedical Materials

Self Cite

View full text Add to dashboard Cite

show abstract

“…Zn ++ may causes interaction with MDP forming Zn-MDP complexes when it is combined with the primer (SEB·P-Zn doped) and applied on dentin previous to the adhesive bonding placement (SEB·Bd). This reduced the Ca-MDP salts formation (Osorio et al, 2011). As a consequence, the penetration of free MDP into the partially demineralized dentin is compromised, due to simultaneous formation of MDP-Zn and Ca-MDP-Zn salts rather than MDP-Ca (Feitosa et al, 2014).…”

Section: Zn-doped Self-etching Adhesivesmentioning

confidence: 99%

“…These Ca-MDP complexes, organized in nano-layering (Yoshida et al, 2012) did not hamper the inward diffusion of ions. These nano-layers helped for further interactions between the remaining Ca ++ and Zn ++ ions, the curable resin matrix containing acidic functional, Zn-MDP and Ca-MDP-Zn complexes, and the partially demineralized collagen (Osorio et al, 2011). This lower salt formation, plus the alkalinity promoted after mechanical loading (McAllister and Frangos, 1999) could also account for the less effective liberation of zinc ions at the resin-dentin interface.…”

mentioning

confidence: 99%

“…This means that the sealing capability was not totally achieved by doping with ZnCl2. ZnCl2 is highly acidic (Brown, 2006) soluble and hydrophilic, and added to the adhesive blend, produces and over-etching effect within the infiltrated dentin, demineralizing even more the underlying carious dentin, just previously and pathologically demineralized (Osorio et al, 2011). Apart from that, it has been clearly demonstrated that intermittent compressive load stimulates the alkaline phosphatase activity.…”

mentioning

confidence: 99%

See 1 more Smart Citation

A zinc chloride-doped adhesive facilitates sealing at the dentin interface: A confocal laser microscopy study

Toledano

Osorio

et al. 2017

Journal of the Mechanical Behavior of Biomedical Materials

Self Cite

View full text Add to dashboard Cite

The aim of this study was to understand the effect of Zn-doping of adhesives and mechanical load cycling on the micromorphology of the resin-dentin interdiffusion zone (of sound and caries affected dentin). The investigation considered two different Zndoped adhesive approaches and evaluated the interface using confocal laser scanning microscopy. Sound and carious dentin-resin interfaces of unloaded specimens were deficiently resin-hybridized, in general. These samples showed a rhodamine B-labeled hybrid layer and adhesive layer completely affected by fluorescein penetration (nanoleakage) through the porous resin-dentin interface, but thicker after phosphoric acid-etching and more extended in carious dentin. Zn-doping promoted an improved sealing of the resin-dentin interface at dentin, a decrease of the hybrid layer porosity, and an increment of dentin mineralization. Load cycled augmented the sealing of the Zn-doped resin-dentin interfaces, as porosity and nanoleakage diminished, and even disappeared in caries-affected dentin substrata conditioned with EDTA. Sound and carious dentin specimens treated with the xylenol orange technique produced a clearly outlined fluorescence when resins were Zn-doped, due to a consistent Ca-mineral deposits within the bonding interface and inside the dentinal tubules, especially when load cycling was applied on specimens treated with Zn-doped bonding components of self-etching adhesives. Micropermeability at the resin-dentin interface diminished after combining EDTA pretreatment, ZnCl2-doping and mechanical loading stimuli on restorations. It is clearly preferable to include the zinc compounds into the bonding constituents of the self-etching adhesives, instead of into the primer ingredients. The promoted new mineral segments contributed to reduce or avoid both porosity and nanoleakage from the load cycled Zn-doped resin dentin interface.

show abstract

“…By scanning electron microscope and energy dispersive X-ray analysis of ZnO-doped adhesive bonded dentin surfaces, it was found that ZnO particles penetrate dentinal tubules, but preferentially remained at the bottom of the hybrid layer. They do not penetrate intertubular dentin but remain in direct contact with the demineralized dentin collagen 14 . The deposition of zinc particles at this site will facilitate the effective release of zinc ions and binding to collagen, at the resin-dentin interface.…”

Section: Introductionmentioning

confidence: 99%

Mechanical loading influences the viscoelastic performance of the resin-carious dentin complex

et al. 2017

Self Cite

View full text Add to dashboard Cite

The aim of this study was to evaluate the changes in the mechanical behavior and bonding capability of Zn-doped resin-infiltrated caries-affected dentin interfaces. Dentin surfaces were treated with 37% phosphoric acid (PA) followed by application of a dentin adhesive, Single Bond (SB) (PA+SB) or by 0.5 M ethylenediaminetetraacetic acid (EDTA) followed by SB (EDTA+SB). 10 wt% ZnO microparticles or 2 wt% ZnCl2 were added into SB, resulting in the following groups: PA+SB, PA+SB-ZnO, PA+SB-ZnCl2, EDTA+SB, EDTA+SB-ZnO, EDTA+SB-ZnCl2. Bonded interfaces were stored for 24 h, and tested or submitted to mechanical loading. Microtensile bond strength was assessed. Debonded surfaces were evaluated by scanning electron microscopy and elemental analysis. The hybrid layer, bottom of the hybrid layer, peritubular and intertubular dentin were evaluated using a nanoindenter. The load/displacement responses were used for the nano-dynamic mechanical analysis (nano-DMA III) to estimate complex modulus, tan delta, loss modulus and storage modulus. The modulus mapping was obtained by imposing a quasistatic force setpoint to which a sinusoidal force was superimposed. AFM imaging was performed. Load cycling decreased the tan delta at the PA+SB-ZnCl2 and EDTA+SB-ZnO interfaces. Tan delta was also diminished at peritubular dentin when PA+SB-ZnO was used, hindering the dissipation of energy throughout these structures. Tan delta increased at the interface after using EDTA+SB-ZnCl2, lowering the energy for recoil or failure. After load cycling, loss moduli at the interface decreased when using ZnCl2 as doping agent, increasing the risk of fracture; but when using ZnO loss moduli was dissimilarly affected if dentin was EDTA-treated. The border between intertubular and peritubular dentin attained the highest discrepancy in values of viscoelastic properties, meaning a risk for cracking and breakdown of the resin-dentin interface. PA used on dentin provoked differences in 4 complex and storage modulus values at the intertubular and peritubular structures, and these differences were higher than when EDTA was employed. In these cases, the longterm performance of the restorative interface will be impared.

show abstract

Zinc-doped dentin adhesive for collagen protection at the hybrid layer

Cited by 62 publications

References 41 publications

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

A zinc chloride-doped adhesive facilitates sealing at the dentin interface: A confocal laser microscopy study

Mechanical loading influences the viscoelastic performance of the resin-carious dentin complex

Contact Info

Product

Resources

About