Zinc enhances CDKN2A , pRb1 expression and regulates functional apoptosis via upregulation of p53 and p21 expression in human breast cancer MCF-7 cell

Al-Saran, Nada; Subash-Babu, Pandurangan; Al-Nouri, Doha M.; Alfawaz, Hanan; Alshatwi, Ali A.

doi:10.1016/j.etap.2016.08.002

Cited by 27 publications

(16 citation statements)

References 44 publications

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“…Zinc plays dual roles in regulating cell proliferation and apoptosis. In vitro cytotoxicity of zinc towards solid tumor cells, especially prostate [43] or breast cancer cells [44], has been widely reported. Recently, apoptosis induced by zinc depletion was also recognized as a potential therapeutic strategy against cancer [45, 46].…”

Section: Discussionmentioning

confidence: 99%

Zinc Depletion by TPEN Induces Apoptosis in Human Acute Promyelocytic NB4 Cells

Zhu

Wang

Zhou

et al. 2017

Cell Physiol Biochem

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Background/Aims: The effects of zinc signaling on proliferation or apoptosis of leukemia cells remain elusive. In the present study, we used N, N, N’, N’-tetrakis-(2-pyridylmethyl)-ethylene-diamine (TPEN), a membrane-permeable zinc chelator, to evaluate the effect of zinc depletion on survival and apoptosis of NB4 acute promyelocytic leukemia (APL) cells. Methods: The pro-apoptotic effects of TPEN on NB4 cells were examined by flow cytometry, and observed using an optical microscope. Intracellular labile zinc, nitric oxide (NO) or reactive oxygen species (ROS) changes caused by TPEN were measured by flow cytometry. We then explored possible roles of the crosstalk between intracellular labile zinc signaling and nitric oxide signaling in TPEN-triggered apoptosis. Results: we found that TPEN induced apoptosis in NB4 APL cells in a dosage-dependent manner. We further demonstrated that TPEN triggered apoptosis by attenuating intracellular zinc and nitric oxide signaling in NB4 cells. Both exogenous zinc supplement and the nitric donor sodium nitroprusside (SNP) pre-incubation reversed TPEN-mediated inhibition of intracellular NO and Zn²⁺ signaling, and rescued NB4 cells from apoptosis. Conclusion: These results suggest for the first time that crosstalk between zinc signaling and nitric oxide pathway is essential for the survival of NB4 cells. TPEN induces apoptosis in NB4 cells via negatively regulating intracellular NO and Zn²⁺ signaling. Our in vitro data suggest that zinc depletion by TPEN may be a potential therapeutic strategy for APL.

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Section: Discussionmentioning

confidence: 99%

Zinc Depletion by TPEN Induces Apoptosis in Human Acute Promyelocytic NB4 Cells

Zhu

Wang

Zhou

et al. 2017

Cell Physiol Biochem

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“…p53 plays an important role in biological processes and cellular components, participates in the process of apoptosis and plays a positive regulatory role in the apoptotic signal pathway. Al-Saran et al (2016) found that zinc can induce apoptosis of human breast cancer MCF-7 cells by up-regulating the expression of p53 and p21. Genomic data analysis found that p53 can affect the incidence of cancer (Stracquadanio et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Establishment of optimal regulatory network of colorectal cancer based on p42.3 protein

Hao¹,

Zhang²,

Shan³

et al. 2017

Saudi Journal of Biological Sciences

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to establish regulatory network of colorectal cancer involving p42.3 protein and to provide theoretical evidence for deep functional exploration of p42.3 protein in the onset and development of colorectal cancer. with protein similarity algorithm, reference protein set of p42.3 cell apoptosis was built according to structural features of p42.3. GO and KEGG databases were used to establish regulatory network of tumor cell apoptosis involving p42.3; meanwhile, the largest possible working pathway that involves p42.3 protein was screened out based on Bayesian network theory. Besides, GO and KEGG were used to build regulatory network on early diagnosis gene markers for colorectal cancer including WWOX, K-ras, COX-2, p53, APC, DCC and PTEN, at the same time, a regulatory network of colorectal cancer cell apoptosis which involves p42.3 was established. cell apoptotic regulatory network that p42.3 participates in primarily consists of Bcl-2 family genes and the largest possible pathway is p42.3 → FKBP → Bcl-2 centered as FKBP protein. Combined with colorectal cancer regulatory network that involves early diagnosis gene markers, it can be predicted that p42.3 is most likely to regulate the colorectal cancer cell apoptosis through FKBP → Bcl-2 → Bax → caspase-9 → caspase-3 pathway. the colorectal cancer apoptosis network based on p42.3 established in the study provides theoretical evidence for deep exploration of p42.3 regulatory mechanism and molecular targeting treatment of colorectal cancer.

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“…Zinc is found in abundance in egg yolks, meat, poultry, legumes, peanuts, non-fat milk powder, cheddar cheese, wheat, and cocoa [125]. It has been proposed that zinc intake might be essential to protect against breast cancer progression and recurrence [126]. According to a recent study, zinc deficiency promoted the abnormal growth of MCF-7 breast cancer cells, and cell exposure to zinc supplements increased the expression of two important genes namely CDKN2A and p53 , and decreased the expression of mdm2 [126].…”

Section: Mediterranean Diet and Breast Cancermentioning

confidence: 99%

The Mediterranean Diet and Breast Cancer: A Personalised Approach

2019

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There have been many original and review articles summarizing the impact of nutrition and diet on breast cancer risk. However, very few consider the implication of genetic background and the effect of personalised nutrition on the risk and prognosis of breast cancer. A literature search was performed using the following databases: MEDLINE (Ovid), PubMed, Scopus and EMBASE (Ovid). The ensuing search terms were selected: genomics, nutrigenomics, breast cancer, breast neoplasms, cancer, nutrigenetics, diet–gene interaction, and Mediterranean, nutrition, polyphenols and diet. In this review, we discuss the Mediterranean-style diet and associated nutrients, evidence of benefit, impact on gene expression and evidence of interactions with genotype and how this interaction can modify breast cancer risk and progression. In addition, the impact of nutrients commonly associated with a Mediterranean-style diet, on breast cancer treatment, and synergistic effects are mentioned when modified by genotype. Some evidence exists around the benefit of a gene-based personalised diet based on a Mediterranean-style dietary pattern, but further evidence in the form of clinical trials is required before such an approach can be comprehensively implemented.

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Zinc enhances CDKN2A , pRb1 expression and regulates functional apoptosis via upregulation of p53 and p21 expression in human breast cancer MCF-7 cell

Cited by 27 publications

References 44 publications

Zinc Depletion by TPEN Induces Apoptosis in Human Acute Promyelocytic NB4 Cells

Zinc Depletion by TPEN Induces Apoptosis in Human Acute Promyelocytic NB4 Cells

Establishment of optimal regulatory network of colorectal cancer based on p42.3 protein

The Mediterranean Diet and Breast Cancer: A Personalised Approach

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