Zinc finger‐containing glycine‐rich RNA‐binding protein in <i>Oryza sativa</i> has an RNA chaperone activity under cold stress conditions

Kim, Joo Y.; Kim, Won Yong; Kwak, Kyung Jin; Oh, Seung Han; Han, Yeon Soo; Kang, Hunseung

doi:10.1111/j.1365-3040.2009.02101.x

Cited by 64 publications

(58 citation statements)

References 31 publications

(98 reference statements)

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“…AtGRP7 is abundantly expressed in guard cells and is important for regulating stomatal movement during dehydration and salt stress . GRPs have been shown to function as RNA chaperones during adaptation to cold stress in both monocots and dicots (Kim et al, 2009;. These studies exemplify the myriad functional roles of plant RBPs during normal plant development and in response to environmental perturbations.…”

Section: Introductionmentioning

confidence: 89%

Phylogenetic and Expression Analysis of RNA-binding Proteins with Triple RNA Recognition Motifs in Plants

Peal¹,

Jambunathan²,

Mahalingam³

2011

Molecules and Cells

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The superfamily of RNA binding proteins (RBPs) is vastly expanded in plants compared to other eukaryotes. A subfamily of RBPs that contain three RNA recognition motifs (RRMs) from the Arabidopsis (24), rice (19) and poplar (37) genomes was analyzed in this study. Phylogenetic analysis with full-length protein sequences of 80 RBPs identified nine clades. The largest clade, comprising 23 members, showed high homology to human RBPs involved in oxidative signaling. Digital northern analysis revealed that Arabidopsis RBPs are transcriptionally responsive to biotic, abiotic and hormonal treatments. Northern blot analysis of eight Arabidopsis RBPs belonging to the tobacco RBP45/47 family showed that these genes respond to ozone stress. AtRBP45b, which shows closest homology to the yeast oxidative stress regulatory protein, CSX1, was expressed in multiple tissues. Two novel splice variant forms of AtRBP45b were identified by 3'RACE analysis. Based on RT-PCR, splice variant AtRBP45b-SV1 was observed only in response to mechanical wounding caused by pathogen or chemical infiltrations and was not detectable in response to salt or temperature stress. Electrophoretic mobility shift assay demonstrated that recombinant full-length and splice variant forms of AtRBP45b bound synthetic RNA. Identifying in vivo RNA targets of AtRBP45b will aid in determining the precise functional role of these proteins during oxidative signaling.

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Section: Introductionmentioning

confidence: 89%

Phylogenetic and Expression Analysis of RNA-binding Proteins with Triple RNA Recognition Motifs in Plants

Peal¹,

Jambunathan²,

Mahalingam³

2011

Molecules and Cells

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“…We have also shown that AtRZ-1a affects negatively seed germination and seedling growth of Arabidopsis under salt or dehydration stress [25]. In recent, the functional roles and RNA chaperone activity of OsRZ proteins in Oryza sativa have been determined during cold adaptation process [26].…”

Section: Introductionmentioning

confidence: 98%

Comparative analysis of Arabidopsis zinc finger-containing glycine-rich RNA-binding proteins during cold adaptation

Kim

Jung

et al. 2010

Plant Physiology and Biochemistry

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“…One GRP family member, designated RZ proteins, harbors CCHC-type zinc knuckles between the RRM and the glycine-rich region. The functional roles of RZs in plant response to different stresses have been determined in diverse plant species, including Arabidopsis thaliana Kim and Kang 2006;Kim et al 2007aKim et al , 2010a and rice (Oryza sativa) (Kim et al 2010b). In particular, certain family members of RZs have been determined to exert their roles by acting as RNA chaperones (Kim et al , 2010b.…”

Section: Introductionmentioning

confidence: 99%

Wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding protein TaRZ1 affects plant growth and defense response in Arabidopsis thaliana

Lee

et al. 2014

Plant Growth Regul

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Although the importance of zinc finger-containing glycine-rich RNA-binding proteins (RZs) during plant growth and stress adaptation has been determined in diverse plant species, the function and importance of RZs in wheat (Triticum aestivum) are largely unknown. Here, functional role of wheat TaRZ1 in the growth and defense response was evaluated using transgenic Arabidopsis thaliana. Ectopic expression of TaRZ1 in Arabidopsis resulted in retarded plant growth and reduced seed yield under normal growth conditions. Moreover, TaRZ1-expressing transgenic plants displayed increased resistance against the pathogenic bacteria Pseudomonas syringae DC3000. Microarray analysis showed that many genes related to plant growth, metabolism, and stress responses are differently up-or down-regulated in Arabidopsis by TaRZ1 expression. Taken together, these results reveal that wheat TaRZ1 has the ability to inhibit the growth of pathogenic bacteria and Arabidopsis, and suggest that TaRZ1 plays diverse roles in the growth and defense of plants.

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Zinc finger‐containing glycine‐rich RNA‐binding protein in Oryza sativa has an RNA chaperone activity under cold stress conditions

Cited by 64 publications

References 31 publications

Phylogenetic and Expression Analysis of RNA-binding Proteins with Triple RNA Recognition Motifs in Plants

Phylogenetic and Expression Analysis of RNA-binding Proteins with Triple RNA Recognition Motifs in Plants

Comparative analysis of Arabidopsis zinc finger-containing glycine-rich RNA-binding proteins during cold adaptation

Wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding protein TaRZ1 affects plant growth and defense response in Arabidopsis thaliana

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