Comparative analysis of Arabidopsis zinc finger-containing glycine-rich RNA-binding proteins during cold adaptation

Kim, Won Yong; Kim, Joo Yeol; Jung, Hyun Ju; Oh, Seung Han; Han, Yeon Soo; Kang, Hunseung

doi:10.1016/j.plaphy.2010.08.013

Cited by 37 publications

(26 citation statements)

References 35 publications

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“…Homologs of RZ-1 in Arabidopsis were shown to have RNA chaperone activity in Escherichia coli (Kim et al, 2005). Overexpression of RZ-1A, but not homologous genes RZ-1B and RZ-1C, confers freezing tolerance in transgenic Arabidopsis (Kim et al, 2007;Kim et al, 2010a;Kim et al, 2010b). However, there is no direct genetic evidence to support the apparently important roles of these GRPs in plant growth and development and in responses to environmental stimuli.…”

Section: Introductionmentioning

confidence: 99%

RNA Binding Proteins RZ-1B and RZ-1C Play Critical Roles in Regulating Pre-mRNA Splicing and Gene Expression during Development in Arabidopsis

Zhu

Lin

et al. 2015

Plant Cell

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Nuclear-localized RNA binding proteins are involved in various aspects of RNA metabolism, which in turn modulates gene expression. However, the functions of nuclear-localized RNA binding proteins in plants are poorly understood. Here, we report the functions of two proteins containing RNA recognition motifs, RZ-1B and RZ-1C, in Arabidopsis thaliana. RZ-1B and RZ-1C were localized to nuclear speckles and interacted with a spectrum of serine/arginine-rich (SR) proteins through their C termini. RZ-1C preferentially bound to purine-rich RNA sequences in vitro through its N-terminal RNA recognition motif. Disrupting the RNA binding activity of RZ-1C with SR proteins through overexpression of the C terminus of RZ-1C conferred defective phenotypes similar to those observed in rz-1b rz-1c double mutants, including delayed seed germination, reduced stature, and serrated leaves. Loss of function of RZ-1B and RZ-1C was accompanied by defective splicing of many genes and global perturbation of gene expression. In addition, we found that RZ-1C directly targeted FLOWERING LOCUS C (FLC), promoting efficient splicing of FLC introns and likely also repressing FLC transcription. Our findings highlight the critical role of RZ-1B/1C in regulating RNA splicing, gene expression, and many key aspects of plant development via interaction with proteins including SR proteins.

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Section: Introductionmentioning

confidence: 99%

RNA Binding Proteins RZ-1B and RZ-1C Play Critical Roles in Regulating Pre-mRNA Splicing and Gene Expression during Development in Arabidopsis

Zhu

Lin

et al. 2015

Plant Cell

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“…Sequence analysis shows that TaRZ2 is most homologous to AtRZ1-a among the three AtRZs (Figure S1). Importantly, the cellular localization of TaRZ2 and AtRZ-1a was similar in that they were localized to the nucleus and cytoplasm, which is different from the nucleus localization of other Arabidopsis [9] and wheat TaRZs (Figure 2). These present and previous results suggest that AtRZ-1a and TaRZ2 are functionally conserved in Arabidopsis and wheat.…”

Section: Discussionmentioning

confidence: 75%

“…It has been reported that, among the three Arabidopsis AtRZs, AtRZ-1a plays a positive role in plant response to cold and freezing stress but negatively affects seed germination and seedling growth of Arabidopsis under salt or dehydration stress [8], [11], [12]. By contrast, AtRZ-1b or AtRZ-1c does not affect seed germination and seedling growth of Arabidopsis under stress conditions [9]. Here, we demonstrated that wheat TaRZ2 contributes to enhance freeze tolerance of Arabidopsis (Figure 5) and plays a negative role during seed germination and seedling growth under salt or dehydration stress conditions (Figure 3 and 4).…”

Section: Discussionmentioning

confidence: 99%

“…Arabidopsis AtRZ-1a is strongly up-regulated by cold stress and down-regulated by dehydration stress or exogenous abscisic acid [8], and affects seed germination and seedling growth of plants under salt, dehydration, or cold stress [8], [11], [12]. In comparison, neither loss-of-function nor overexpression of AtRZ-1b or AtRZ-1c affects seed germination and seedling growth of plants under stress [9]. The rice genome encodes three RZ genes, named OsRZ1, OsRZ2, and OsRZ3.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Functional Analysis of Wheat (Triticum aestivum) Zinc Finger-Containing Glycine-Rich RNA-Binding Proteins in Response to Abiotic Stresses

Choi

et al. 2014

PLoS ONE

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Although the functional roles of zinc finger-containing glycine-rich RNA-binding proteins (RZs) have been characterized in several plant species, including Arabidopsis thaliana and rice (Oryza sativa), the physiological functions of RZs in wheat (Triticum aestivum) remain largely unknown. Here, the functional roles of the three wheat RZ family members, named TaRZ1, TaRZ2, and TaRZ3, were investigated using transgenic Arabidopsis plants under various abiotic stress conditions. Expression of TaRZs was markedly regulated by salt, dehydration, or cold stress. The TaRZ1 and TaRZ3 proteins were localized to the nucleus, whereas the TaRZ2 protein was localized to the nucleus, endoplasmic reticulum, and cytoplasm. Germination of all three TaRZ-expressing transgenic Arabidopsis seeds was retarded compared with that of wild-type seeds under salt stress conditions, whereas germination of TaRZ2- or TaRZ3-expressing transgenic Arabidopsis seeds was retarded under dehydration stress conditions. Seedling growth of TaRZ1-expressing transgenic plants was severely inhibited under cold or salt stress conditions, and seedling growth of TaRZ2-expressing plants was inhibited under salt stress conditions. By contrast, expression of TaRZ3 did not affect seedling growth of transgenic plants under any of the stress conditions. In addition, expression of TaRZ2 conferred freeze tolerance in Arabidopsis. Taken together, these results suggest that different TaRZ family members play various roles in seed germination, seedling growth, and freeze tolerance in plants under abiotic stress.

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“…One GRP family member, designated RZ proteins, harbors CCHC-type zinc knuckles between the RRM and the glycine-rich region. The functional roles of RZs in plant response to different stresses have been determined in diverse plant species, including Arabidopsis thaliana Kim and Kang 2006;Kim et al 2007aKim et al , 2010a and rice (Oryza sativa) (Kim et al 2010b). In particular, certain family members of RZs have been determined to exert their roles by acting as RNA chaperones (Kim et al , 2010b.…”

Section: Introductionmentioning

confidence: 99%

Wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding protein TaRZ1 affects plant growth and defense response in Arabidopsis thaliana

Lee

et al. 2014

Plant Growth Regul

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Although the importance of zinc finger-containing glycine-rich RNA-binding proteins (RZs) during plant growth and stress adaptation has been determined in diverse plant species, the function and importance of RZs in wheat (Triticum aestivum) are largely unknown. Here, functional role of wheat TaRZ1 in the growth and defense response was evaluated using transgenic Arabidopsis thaliana. Ectopic expression of TaRZ1 in Arabidopsis resulted in retarded plant growth and reduced seed yield under normal growth conditions. Moreover, TaRZ1-expressing transgenic plants displayed increased resistance against the pathogenic bacteria Pseudomonas syringae DC3000. Microarray analysis showed that many genes related to plant growth, metabolism, and stress responses are differently up-or down-regulated in Arabidopsis by TaRZ1 expression. Taken together, these results reveal that wheat TaRZ1 has the ability to inhibit the growth of pathogenic bacteria and Arabidopsis, and suggest that TaRZ1 plays diverse roles in the growth and defense of plants.

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Comparative analysis of Arabidopsis zinc finger-containing glycine-rich RNA-binding proteins during cold adaptation

Cited by 37 publications

References 35 publications

RNA Binding Proteins RZ-1B and RZ-1C Play Critical Roles in Regulating Pre-mRNA Splicing and Gene Expression during Development in Arabidopsis

RNA Binding Proteins RZ-1B and RZ-1C Play Critical Roles in Regulating Pre-mRNA Splicing and Gene Expression during Development in Arabidopsis

Comparative Functional Analysis of Wheat (Triticum aestivum) Zinc Finger-Containing Glycine-Rich RNA-Binding Proteins in Response to Abiotic Stresses

Wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding protein TaRZ1 affects plant growth and defense response in Arabidopsis thaliana

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