Zinc induces distinct changes in the metabolism of reactive oxygen and nitrogen species (ROS and RNS) in the roots of two<i>Brassica</i>species with different sensitivity to zinc stress

Feigl, Gábor; Lehotai, Nóra; Molnár, Árpád; Ördög, Attila; Rodríguez-Ruiz, Marta; Palma, José M.; Corpas, Francisco J.; Erdei, László; Kolbert, Zsuzsanna

doi:10.1093/aob/mcu246

Cited by 118 publications

(72 citation statements)

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“…Plants undergoing metal stress result in series of signaling events that largely includes reprogramming of transcripts that could help them to overcome the toxicity. In plants, excess of Zn also results in the generation of reactive oxygen and nitrogen species (Feigl et al, 2015). The abundance of multiple membrane proteins is increased by the presence of either excess Zn or Fe deficiency.…”

Section: Discussionmentioning

confidence: 99%

Overlapping transcriptional expression response of wheat zinc-induced facilitator-like transporters emphasize important role during Fe and Zn stress

Sharma

Kaur

Kumar

et al. 2019

Preprint

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BackgroundHexaploid wheat is an important cereal crop that has been targeted to enhance grain micronutrient content including zinc and iron. In this direction, modulating the expression of plant transporters involved in Fe and Zn homeostasis could be one of the promising approaches. Therefore, the present work was undertaken to identify bread wheatZinc–InducedFacilitator-Like (ZIFL) family of transporters and study their transcriptional expression response during micronutrient fluctuations and exposure to multiple heavy metals.ResultsThe genome-wide analyses resulted in identification of thirty-five putativeTaZIFLgenes, which were distributed only on Chromosome 3, 4 and 5. Wheat ZIFL proteins subjected to the phylogenetic analysis showed the uniform distribution along with rice,Arabidopsisand maize.In-silicoanalysis of the promoters of the wheat ZIFL genes suggested the presence of multiple metal binding sites including those which are involved in Fe homeostasis. QRT-PCR analysis of wheatZIFLgenes suggested the differential regulation of the transcripts in roots and shoots under surplus Zn and also during Fe starvation. Specifically, in roots,TaZIFL2.3, TaZIFL4.1, TaZIFL4.2, TaZIFL5, TaZIFL6.1andTaZIFL6.2were significantly up-regulated by both Zn and Fe. This suggested that ZIFL could possibly be regulated by both the nutrient stress in a tissue specific manner. Interestingly, upon exposure to heavy metals,TaZIFL4.2andTaZIFL7.1showed significant up-regulation, whereasTaZIFL5andTaZIFL6.2remained almost unaffected.ConclusionThis is the first report with detailed analysis of wheat ZIFL genes. Our study also identifies closest ortholog for transporter of mugineic acid, a chelator required for Fe uptake. Comprehensive transcript expression pattern during development of wheat seedlings and against various abiotic/biotic stresses resulted in tissue specific responses. Overall, this work addresses the role of wheatZIFLduring the interplay between micronutrient and heavy metal stress in a tissue specific manner.

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Section: Discussionmentioning

confidence: 99%

Overlapping transcriptional expression response of wheat zinc-induced facilitator-like transporters emphasize important role during Fe and Zn stress

Sharma

Kaur

Kumar

et al. 2019

Preprint

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“…In plants, lipid oxidation is not a phenomenon restricted to seed aging; instead, it plays a role in many processes, like photooxidative stress (Broin and Rey, 2003), drought-induced leaf damage (Avramova et al, 2017), and metal-induced root damage (Feigl et al, 2015). Also, numerous studies in the fields of microbiology (Imlay, 2013) and medicine (Ames et al, 1993) link lipid oxidation to biological questions of great relevance, like pathogen defense, aging, and cardiovascular diseases.…”

Section: Discussionmentioning

confidence: 99%

Structure Annotation and Quantification of Wheat Seed Oxidized Lipids by High-Resolution LC-MS/MS

2017

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Lipid oxidation is a process ubiquitous in life, but the direct and comprehensive analysis of oxidized lipids has been limited by available analytical methods. We applied high-resolution liquid chromatography-mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) to quantify oxidized lipids (glycerides, fatty acids, phospholipids, lysophospholipids, and galactolipids) and implemented a platform-independent high-throughput-amenable analysis pipeline for the high-confidence annotation and acyl composition analysis of oxidized lipids. Lipid contents of 90 different naturally aged wheat (Triticum aestivum) seed stocks were quantified in an untargeted high-resolution LC-MS experiment, resulting in 18,556 quantitative mass-to-charge ratio features. In a posthoc liquid chromatography-tandem mass spectrometry experiment, high-resolution MS/MS spectra (5 mD accuracy) were recorded for 8,957 out of 12,080 putatively monoisotopic features of the LC-MS data set. A total of 353 nonoxidized and 559 oxidized lipids with up to four additional oxygen atoms were annotated based on the accurate mass recordings (1.5 ppm tolerance) of the LC-MS data set and filtering procedures. MS/MS spectra available for 828 of these annotations were analyzed by translating experimentally known fragmentation rules of lipids into the fragmentation of oxidized lipids. This led to the identification of 259 nonoxidized and 365 oxidized lipids by both accurate mass and MS/MS spectra and to the determination of acyl compositions for 221 nonoxidized and 295 oxidized lipids. Analysis of 15-year aged wheat seeds revealed increased lipid oxidation and hydrolysis in seeds stored in ambient versus cold conditions. Lipid oxidation is a process inevitably connected to life. Although essential for all respiring organisms, oxygen can form reactive species (ROS). The accumulation of these compounds leads to the phenomenon commonly referred to as oxidative stress in microbes and plant and animal tissues. ROS play a role in the attack against microbial pathogens by host cells (Imlay, 2013), time-dependent death of dormant seeds (Lee et al., 2010), human diseases (Coyle and Puttfarcken, 1993;Giugliano et al., 1996), in aging (Sohal and Weindruch, 1996;Sohal et al., 2002), and in signaling processes (Gilroy et al., 2016;Mignolet-Spruyt et al., 2016) Unlike oxidized fatty acids (FAs), oxidized lipids are hardly available commercially. Targeted liquid chromatography-mass spectrometry (LC-MS) analysis of oxidized lipids using authentic standards is not very common (Hui et al., 2010;Ravandi et al., 2014). Often, oxidized lipids are detected using tandem mass spectrometry (MS/MS), where the collision-induced products of precursor ions (PRs) are scanned for fragments or neutral losses (NLs) that correspond to oxidized acyl residues expected in oxidized lipids (Spickett and Pitt, 2015), and in some cases, higher resolution MS/MS spectra are provided to support the identification (Buseman et al., 2006;Vu et al., 2012).High-resolution mass spectrometry (MS) allows for ...

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“…for photosynthesis. Feigl et al (2015) reported that a Zn treatment significantly increased the Cu and Fe contents but decreased the Mn content in the roots of Brassica, compared with the control. Zn and Cu use the same transporters, which can be upregulated by excess Zn, although Cu is preferred (Fraústo da Silva & Williams, 2001).…”

Section: Effects Of Zn Treatments On Volume and Zn Content Of Guttatimentioning

confidence: 98%

Hydathode function and changes in contents of elements in eddo exposed to zinc in hydroponic solution

Hossain

Sawada

Noda

et al. 2017

Plant Production Science

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Zinc induces distinct changes in the metabolism of reactive oxygen and nitrogen species (ROS and RNS) in the roots of twoBrassicaspecies with different sensitivity to zinc stress

Cited by 118 publications

References 69 publications

Overlapping transcriptional expression response of wheat zinc-induced facilitator-like transporters emphasize important role during Fe and Zn stress

Overlapping transcriptional expression response of wheat zinc-induced facilitator-like transporters emphasize important role during Fe and Zn stress

Structure Annotation and Quantification of Wheat Seed Oxidized Lipids by High-Resolution LC-MS/MS

Hydathode function and changes in contents of elements in eddo exposed to zinc in hydroponic solution

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