Zinc overload mediated by zinc oxide nanoparticles as innovative anti-tumor agent

Wiesmann, Nadine; Kluenker, Martin; Demuth, Philipp; Brenner, Walburgis; Tremel, Wolfgang; Brieger, Juergen

doi:10.1016/j.jtemb.2018.08.002

Cited by 57 publications

(27 citation statements)

References 33 publications

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“…Several studies have shown that the cytotoxicity of ZnO-NPs results from the generation of ROS [20][21][22]. We first used the ROS/superoxide detection kit to determine the impact of ZnO-NPs on oxidative stress in gingival cancer cells.…”

Section: Zno-nps Stimulated Ros and Superoxide Generation In Gingivalmentioning

confidence: 99%

ZnO Nanoparticles Induced Caspase-Dependent Apoptosis in Gingival Squamous Cell Carcinoma through Mitochondrial Dysfunction and p70S6K Signaling Pathway

Wang

Lee

Lin

et al. 2020

IJMS

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Zinc oxide nanoparticles (ZnO-NPs) are increasingly used in sunscreens, food additives, pigments, rubber manufacture, and electronic materials. Several studies have shown that ZnO-NPs inhibit cell growth and induce apoptosis by the production of oxidative stress in a variety of human cancer cells. However, the anti-cancer property and molecular mechanism of ZnO-NPs in human gingival squamous cell carcinoma (GSCC) are not fully understood. In this study, we found that ZnO-NPs induced growth inhibition of GSCC (Ca9-22 and OECM-1 cells), but no damage in human normal keratinocytes (HaCaT cells) and gingival fibroblasts (HGF-1 cells). ZnO-NPs caused apoptotic cell death of GSCC in a concentration-dependent manner by the quantitative assessment of oligonucleosomal DNA fragmentation. Flow cytometric analysis of cell cycle progression revealed that sub-G1 phase accumulation was dramatically induced by ZnO-NPs. In addition, ZnO-NPs increased the intracellular reactive oxygen species and specifically superoxide levels, and also decreased the mitochondrial membrane potential. ZnO-NPs further activated apoptotic cell death via the caspase cascades. Importantly, anti-oxidant and caspase inhibitor clearly prevented ZnO-NP-induced cell death, indicating the fact that superoxide-induced mitochondrial dysfunction is associated with the ZnO-NP-mediated caspase-dependent apoptosis in human GSCC. Moreover, ZnO-NPs significantly inhibited the phosphorylation of ribosomal protein S6 kinase (p70S6K kinase). In a corollary in vivo study, our results demonstrated that ZnO-NPs possessed an anti-cancer effect in a zebrafish xenograft model. Collectively, these results suggest that ZnO-NPs induce apoptosis through the mitochondrial oxidative damage and p70S6K signaling pathway in human GSCC. The present study may provide an experimental basis for ZnO-NPs to be considered as a promising novel anti-tumor agent for the treatment of gingival cancer.

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Section: Zno-nps Stimulated Ros and Superoxide Generation In Gingivalmentioning

confidence: 99%

ZnO Nanoparticles Induced Caspase-Dependent Apoptosis in Gingival Squamous Cell Carcinoma through Mitochondrial Dysfunction and p70S6K Signaling Pathway

Wang

Lee

Lin

et al. 2020

IJMS

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“…Nanoparticles can be used as nanofertilizer for emergent of plants but although a number of researches utter nanoparticles prevent the enlargement of some plants, few others stated the development of some other plants [3,6]. The techniques comprise in the fusion of ZnO nanoparticles (ZnO NPs) are chemical steam toppling, gas stage method, drenched decomposition, hydrothermal mixture, micro suspension, electrochemical technique, thumped laser deposit, microwave blend, and the gel processing [7]. The significant of the produced ZnO has possible characteristics such as antimicrobial, gas detector, photoreactions, and deprivation of organic dyes [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Zinc oxide nanoparticles synthesised from the Vernonia amygdalina shows the anti-inflammatory and antinociceptive activities in the mice model

Liu

Kang

Liu³

et al. 2020

Artificial Cells, Nanomedicine, and Biotechnology

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In this study, we synthesised the zinc oxide nanoparticles from Vernonia amygdalina and evaluated its anti-inflammatory and antinociceptive potentials against the different inflammation and pain induced mice model. The synthesised zinc oxide nanoparticles were characterised by UV, SEM, XRD and FTIR techniques. The anti-nociceptive effects of V. amygdalina were examined by different stimuli e.g. acetic acid, glutamate, capsaicin, and formalin-induced nociception in mice. The anti-inflammatory effects of synthesised zinc oxide nanoparticles were assessed by air sack assessment and the level of inflammatory cytokines were studied. The muscle tension of animals were studied through open field assessment. The present study exhibited proficient antinociceptive and anti-inflammatory actions of the synthesised Zinc oxide nanoparticles from V. amygdalina. The sormulated zinc oxide nanoparticles were appreciably reduced the acetic acid, glutamate, capsaicin, and formalin-induced nociceptive responses in mice. Further the zinc nanoparticles were exhibited the potent anti-inflammatory actions via reducing the inflammatory response and pro-inflammatory cytokines level in the mice. In conclusion, the findings of this study proved the beneficial effects of zinc oxide nanoparticles from V. amygdalina against the different pain and inflammation-induced mice. Hence, it was clear that the zinc nanoparticles from V. amygdalina could be promising antinociceptive and anti-inflammatory agent in the future.

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“…highly inert), whereas oxides of the late 3d transition metals (e.g. Mn, Fe, Co, or Zn) are soluble/reactive under acidic/basic conditions or in the presence of coordinating ligands . As SnO 2 forms a dense layer of aligned lamellae, its uptake by cells is very unlikely.…”

Section: Resultsmentioning

confidence: 99%

Self‐Organized Arrays of SnO₂ Microplates with Photocatalytic and Antimicrobial Properties

et al. 2019

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Porous anodic metal oxides have been widely used for the development of various functional nanostructures. So far, these self-organized pore structures were prepared as hexagonal arrangements of nanopores, which have become a popular template system for the fabrication of functional nanostructures. Here we report a new oxalic acid-based anodization process for long-range ordered arrays of submicrometer tin dioxide plates at low applied voltages in buffered solution, which prevents the precipitation of SnC 2 O 4 on the Sn anode surface. The chemical composition of the surface SnO 2 was established [a]

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Zinc overload mediated by zinc oxide nanoparticles as innovative anti-tumor agent

Cited by 57 publications

References 33 publications

ZnO Nanoparticles Induced Caspase-Dependent Apoptosis in Gingival Squamous Cell Carcinoma through Mitochondrial Dysfunction and p70S6K Signaling Pathway

ZnO Nanoparticles Induced Caspase-Dependent Apoptosis in Gingival Squamous Cell Carcinoma through Mitochondrial Dysfunction and p70S6K Signaling Pathway

Zinc oxide nanoparticles synthesised from the Vernonia amygdalina shows the anti-inflammatory and antinociceptive activities in the mice model

Self‐Organized Arrays of SnO₂ Microplates with Photocatalytic and Antimicrobial Properties

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Zinc overload mediated by zinc oxide nanoparticles as innovative anti-tumor agent

Cited by 57 publications

References 33 publications

ZnO Nanoparticles Induced Caspase-Dependent Apoptosis in Gingival Squamous Cell Carcinoma through Mitochondrial Dysfunction and p70S6K Signaling Pathway

ZnO Nanoparticles Induced Caspase-Dependent Apoptosis in Gingival Squamous Cell Carcinoma through Mitochondrial Dysfunction and p70S6K Signaling Pathway

Zinc oxide nanoparticles synthesised from the Vernonia amygdalina shows the anti-inflammatory and antinociceptive activities in the mice model

Self‐Organized Arrays of SnO2 Microplates with Photocatalytic and Antimicrobial Properties

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Self‐Organized Arrays of SnO₂ Microplates with Photocatalytic and Antimicrobial Properties