Zinc oxide nanoparticles inhibits quorum sensing and virulence in Pseudomonas aeruginosa

Saleh, Moustafa M.; Sadeq, Refat; Latif, Hemat K. Abdel; Abbas, Hisham A.; Askoura, Momen

doi:10.4314/ahs.v19i2.28

Cited by 45 publications

(22 citation statements)

References 27 publications

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“…In accordance with our data, Saleh et al reported that ZnO-np had a signi cant decrease in the relative expression of QS-genes lasI, lasR, rhlI, rhlR, pqsA and pqsR. Additionally, ZnO signi cantly decreased the pathogenesis of P.aeruginosa in vivo [12].…”

Section: Discussionsupporting

confidence: 92%

The effect of Zinc Oxide Nanoparticles on Pseudomonas Aeruginosa Biofilm Formation and Virulence Genes Expression

Abdelraheem

Mohamed

2020

Preprint

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Abstract Background: Due to increased resistance to antimicrobial agents, infectious disease remains a public health problem worldwide. Aim: The current study was designed to examine the effect of ZnO nanoparticles (ZnO –np) against the biofilm formation ability of P.aeruginosa clinical isolates and to study its effect on the expression level of the genes involved in biofilm formation and virulence factors production. Methodology: The MIC of ZnO –np against P. aeruginosa was determined by the broth microdilution method. The Effect of ZnO –np on the biofilm-forming isolates of P. aeruginosa was monitored by the microtiter plate method. P.aeruginosa isolates were tested for the expression of different biofilm and virulence genes using real-time rt-PCR. Results: ZnO –np significantly downregulated the expression level of all biofilm and virulence genes of P.aeruginosa clinical isolates except the toxA gene. Conclusions: This study demonstrates the promising use of ZnO –np as an anti-biofilm and anti-virulence compound.

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Section: Discussionsupporting

confidence: 92%

The effect of Zinc Oxide Nanoparticles on Pseudomonas Aeruginosa Biofilm Formation and Virulence Genes Expression

Abdelraheem

Mohamed

2020

Preprint

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“…Researchers showed that ZnO NPs were able to inhibit QS in P. aeruginosa by down regulating lasR. lasI, rhl I, and rhl R (Saleh et al, 2019). Another study showed that ZnO NPs were able to reduce the swimming and swarming motility within P. aeruginosa and also showed its efficacy against the pqs and las system of QS (Khan et al, 2020).…”

Section: Inhibition Of Quorum Sensing By Micro Biogenic Zno Npsmentioning

confidence: 99%

Microbiologically-Synthesized Nanoparticles and Their Role in Silencing the Biofilm Signaling Cascade

et al. 2021

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The emergence of bacterial resistance to antibiotics has led to the search for alternate antimicrobial treatment strategies. Engineered nanoparticles (NPs) for efficient penetration into a living system have become more common in the world of health and hygiene. The use of microbial enzymes/proteins as a potential reducing agent for synthesizing NPs has increased rapidly in comparison to physical and chemical methods. It is a fast, environmentally safe, and cost-effective approach. Among the biogenic sources, fungi and bacteria are preferred not only for their ability to produce a higher titer of reductase enzyme to convert the ionic forms into their nano forms, but also for their convenience in cultivating and regulating the size and morphology of the synthesized NPs, which can effectively reduce the cost for large-scale manufacturing. Effective penetration through exopolysaccharides of a biofilm matrix enables the NPs to inhibit the bacterial growth. Biofilm is the consortia of sessile groups of microbial cells that are able to adhere to biotic and abiotic surfaces with the help extracellular polymeric substances and glycocalyx. These biofilms cause various chronic diseases and lead to biofouling on medical devices and implants. The NPs penetrate the biofilm and affect the quorum-sensing gene cascades and thereby hamper the cell-to-cell communication mechanism, which inhibits biofilm synthesis. This review focuses on the microbial nano-techniques that were used to produce various metallic and non-metallic nanoparticles and their “signal jamming effects” to inhibit biofilm formation. Detailed analysis and discussion is given to their interactions with various types of signal molecules and the genes responsible for the development of biofilm.

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“…Real-time rt-PCR reaction was prepared with a final volume of 20 µl (master mix: 10 µl, Forward primer: 0.8 µl, Reverse primer: 0.8 µl, Reverse transcriptase: 0.2 µl, RNase inhibitor, 0.4 µl, Water up to 16 µl and Template: 4 µl). Different genes and primers were listed in Table 1 [11][12][13][14]. Four negative control samples containing deionized water instead of template, one for each gene were included in the same PCR run.…”

Section: Rt-pcrmentioning

confidence: 99%

The effect of Zinc Oxide nanoparticles on Pseudomonas aeruginosa biofilm formation and virulence genes expression

Abdelraheem

Mohamed

2021

J Infect Dev Ctries

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Introduction: Due to increased resistance to antimicrobial agents, infectious diseases remain a public health problem worldwide. The current study was designed to examine the effect of Zinc Oxide nanoparticles (ZnO–np) against the biofilm formation ability of P. aeruginosa clinical isolates and to study its effect on the expression level of the genes involved in biofilm formation and virulence factors production. Methodology: The MIC of ZnO–np against P. aeruginosa was determined by the broth micro dilution method. The effect of ZnO–np on the biofilm-forming isolates of P. aeruginosa was monitored by the microtiter plate method. P. aeruginosa isolates were tested for the expression of different biofilm and virulence genes using real-time rt-PCR. Results: ZnO–np significantly down-regulated the expression level of all biofilm and virulence genes of P. aeruginosa clinical isolates except the toxA gene. Conclusions: This study demonstrates the promising use of ZnO–np as an anti-biofilm and anti-virulence compound.

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Zinc oxide nanoparticles inhibits quorum sensing and virulence in Pseudomonas aeruginosa

Cited by 45 publications

References 27 publications

The effect of Zinc Oxide Nanoparticles on Pseudomonas Aeruginosa Biofilm Formation and Virulence Genes Expression

The effect of Zinc Oxide Nanoparticles on Pseudomonas Aeruginosa Biofilm Formation and Virulence Genes Expression

Microbiologically-Synthesized Nanoparticles and Their Role in Silencing the Biofilm Signaling Cascade

The effect of Zinc Oxide nanoparticles on Pseudomonas aeruginosa biofilm formation and virulence genes expression

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