Zinc Oxide Nanoparticles Protected with Terpenoids as a Substance in Redox Imbalance Normalization in Burns

Abstract

Preliminary protection of zinc oxide nanoparticles (ZnO NPs) with terpenoids such as betulin, its derivatives, and essential oils components has been proposed to produce gel-like oleophilic and hydrophilic formulations. We studied the properties of gel-like dispersions of ZnO NPs with immobilized terpenoids and their effects on the activity of LDH, GR, G6PDH, restoration of redox balance of co-enzyme pairs NAD+/NADH and NADP+/NADPH, as well as the activity of SOD, catalase, AlDH in erythrocytes in the treatment of burns in rats. Hysteresis loops on the rheograms of studied dispersions characterize their thixotropic properties. ZnO NPs with betulin diphosphate in the water–ethanol medium lead to a 20-fold increase in the hydrodynamic radius at pH 7.3 compared to the initial ZnO NPs, and facilitate the formation of Zn2+ ions and their penetration into the viable epidermis, unlike oleophilic dispersions. All dispersions reduce the healing time by one and a half times compared with the untreated control group, increase the activity of LDH, GR, G6PDH, SOD, catalase, AlDH, and contribute to the normalization of coenzyme balance. Normalization of the redox balance and wound state was more effective using hydrophilic dispersions due to Zn2 + penetration.

Interaction between neutrophil extracellular traps and cardiomyocytes contributes to atrial fibrillation progression

“…The substantial effect of the TLRs signaling pathway activation on cardiomyocytes remains controversial. 38 – 42 We primarily hypothesized TLR4 signaling was activated by NETs and led to the apoptosis of cardiomyocytes. However, the knockdown of TLR4 resulted in increased NETs-mediated cardiomyocyte death, which demonstrated the protective role of TLR4 in the process (Supplementary Fig.…”

Healing potential of nanocarriers containing essential oils: a literature review

“…Inorganic nanoparticles are biocompatible, protect bioactive from degradation, with a size that can vary according to the core material used (MOHAMMADPOUR et al, 2019). Metallic and semiconductor nanoparticles such as iron oxide (ANGHEL et al, 2013), cobalt (HOU et al, 2020 and zinc oxide (MELNIKOVA et al, 2021) were used incorporating essential oils to assess healing potential.…”

“…Regarding the essential oils evaluated, lavender, eucalyptus and clove were the ones that most had their properties analyzed, in concentrations ranging from 1-5%. Regarding the in vivo forms of administration, the topical route was used (MELNIKOVA et al, 2021;HOU et al, 2020;KHAN et al, 2021;HAJIALI et al, 2015;KALITA et al, 2017;KHEZRI;FARAHPOUR;RAD, 2019;SAPORITO et al, 2018;KAMEL et al, 2022;RIZG et al, 2022;LI et al, 2021;SUGUMAR et al, 2014) and oral (SHAKEEL et al , 2019;ALAM et al, 2017;ALAM et al, 2018).…”

“…The results obtained from the application in rat models was that the use of NPCo ointment in the treatment groups substantially increased (p ≤ 0.01) wound contracture compared to the other groups, proving to be a possible substance to be used for the treatment of various types of skin wounds in humans. Melnikova et al (2021) studied the behavior of terpenoid-protected zinc oxide nanoparticles (ZnO NPs) in the normalization of redox imbalance in burns. Hydrophilic and oleophilic gel dispersions have been developed, incorporating terpenoids from lavender and thyme oil (thymol), and betulin triterpenoids (B) or betulin diphosphate (BDP), due to their known anti-inflammatory and antioxidant properties.…”

The Effect of Betulin Diphosphate in Wound Dressings of Bacterial Cellulose-ZnO NPs on Platelet Aggregation and the Activity of Oxidoreductases Regulated by NAD(P)+/NAD(P)H-Balance in Burns on Rats

“…Earlier, in an experiment on rats, we showed that betulin diphosphate is a promising API for treating burns as a component of wound dressings made of bacterial cellulose and zinc oxide nanoparticles, and various dispersions, including oleogels with vegetable oils [ 28 , 29 ].…”