Zinc oxide nanoparticles reduce biofilm formation, synergize antibiotics action and attenuate Staphylococcus aureus virulence in host; an important message to clinicians

Abdelghafar, Aliaa; Yousef, Nehal; Askoura, Momen

doi:10.1186/s12866-022-02658-z

Cited by 46 publications

(15 citation statements)

References 40 publications

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“…The highest concentration of ZnO NPs (8 μg/disc) exhibited the largest zone of inhibition against both bacterial strains. These findings have implications for the potential use of ZnO NPs as an alternative to conventional antibiotics in the treatment of bacterial infections. ,, However, further studies are required to investigate the mechanism of action of ZnO NPs against bacterial strains and their potential toxicity toward human cells.…”

Section: Resultsmentioning

confidence: 99%

Green Synthesis of Zinc Oxide Nanoparticles Using Cymbopogon citratus Extract and Its Antibacterial Activity

Mohammed,

Alghamdi,

Jabbar

et al. 2023

ACS Omega

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Excessive use of antimicrobial medications including antibiotics has led to the emerging menace of antimicrobial resistance, which, as per the World Health Organization (WHO), is among the top ten public health threats facing humanity, globally. This necessitates that innovative technologies be sought that can aid in the elimination of pathogens and hamper the spread of infections. Zinc oxide (ZnO) has multifunctionality owing to its extraordinary physico-chemical properties and functionality in a range of applications. In this research, ZnO nanoparticles (NPs) were synthesized from zinc nitrate hexahydrate, by a green synthesis approach using Cymbopogon citratus extract followed by characterization of the NPs. The obtained X-ray diffraction peaks of ZnO NPs matched with the standard JCPDS card (no. 89-510). The particles had a size of 20–24 nm, a wurtzite structure with a high crystallinity, and hexagonal rod-like shape. UV–Vis spectroscopy revealed absorption peaks between 369 and 374 nm of ZnO NPs synthesized from C. citratus extract confirming the formation of ZnO. Fourier transform infrared confirmed the ZnO NPs as strong absorption bands were observed in the range of 381–403 cm–1 corresponding to Zn–O bond stretching. Negative values of the highest occupied molecular orbital–lowest unoccupied molecular orbital for ZnO NPs indicated the good potential to form a stable ligand–protein complex. Docking results indicated favorable binding interaction between ZnO and DNA gyrase subunit b with a binding energy of −2.93 kcal/mol. ZnO NPs at various concentrations inhibited the growth of Escherichia coli and Staphylococcus aureus. Minimum inhibitory concentration values of ZnO NPs against E. coli and S. aureus were found to be 92.07 ± 0.13 and 88.13 ± 0.35 μg/mL, respectively, at a concentration of 2 mg/mL. AO/EB staining and fluorescence microscopy revealed the ability of ZnO NPs to kill E. coli and S. aureus cells. Through the findings of this study, it has been shown that C. citratus extract can be used in a green synthesis approach to generate ZnO NPs, which can be employed as alternatives to antibiotics and a tool to eliminate drug-resistant microbes in the future.

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Section: Resultsmentioning

confidence: 99%

Green Synthesis of Zinc Oxide Nanoparticles Using Cymbopogon citratus Extract and Its Antibacterial Activity

Mohammed,

Alghamdi,

Jabbar

et al. 2023

ACS Omega

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“…The formed ROS can irreversibly damage the bacteria membrane, DNA, mitochondria, etc. [22] , [44] . The mechanism of synergism between Prot-K and NPs ZnO_G240 can be inferred from the proteinase K treatment, which significantly degraded the related proteins in the EPS matrix, and the biofilm cells became loose.…”

Section: Resultsmentioning

confidence: 99%

A combined treatment of Proteinase K and biosynthesized ZnO-NPs for eradication of dairy biofilm of sporeformers

et al. 2022

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<abstract> <p>Biofilms of sporeformers found in the dairy industry are the major contaminants during processing, as they withstand heat and chemical treatment that are used to control microbes. The present work is aimed to remove these resistant forms of bacterial community (biofilm) present in dairy production lines using ecofriendly agents based on proteinase K (Prot-K) coupled with Zinc oxide nanoparticles (ZnO-NPs). Some metal/metal oxide (Ag, CuO and ZnO) NPs were prepared microbially, and ZnO-NPs were characterized as the most effective ones among them. The produced ZnO-NPs were 15–25 nm in size with spherical shape, and FTIR analysis confirmed the presence of proteins and alkanes surrounding particles as capping agents. Application of Prot-K for eradication (removal) of a model biofilm of mixed sporeformers on food-grade stainless steel resulted in an 83% reduction in the absorbance of crystal violet-stained biofilm. When Prot-K was mixed with the biosynthesized NPs ZnO_G240, the reduction increased to 99.19%. This finding could contribute to an efficient cleaning approach combined with CIP to remove the recalcitrant biofilms in dairy production lines.</p> </abstract>

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“…ZnO NPs have demonstrated potent antimicrobial properties against a wide range of dental pathogens due to their ability to induce oxidative stress and disrupt microbial cell membranes [13]. Additionally, ZnO NPs have shown potential in inhibiting bio lm formation, a crucial factor in the pathogenesis of dental infections [22]. PIP, on the other hand, possesses antimicrobial properties and has been reported to enhance the bioavailability and e cacy of various drugs [23].…”

Section: Discussionmentioning

confidence: 99%

Targeted piperine-coated zinc oxide nanoparticle induces the biofilm inhibition of dental pathogens and apoptosis of oral cancer through the BCL-2/BAX/P53 signaling pathway

Shaik,

Kandaswamy,

Guru

et al. 2024

Preprint

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Background Dental pathogens, encompassing bacteria, viruses, and fungi, are pivotal in the development of oral health issues such as tooth decay, gum disease, and oral infections. Interestingly, recent research suggests a potential interconnection between dental pathogens and oral cancer, with evidence implicating certain oral pathogens in the initiation and progression of oral malignancies. Given the rising concern of antibiotic resistance and the limitations of conventional treatments, there is a compelling need for innovative therapeutic approaches. Methods The research involved the synthesis and analysis of zinc oxide nanoparticles (ZnO NPs) facilitated by piperine (PIP). Various techniques including UV spectroscopy, SEM, XRD, FTIR, and EDAX were employed to confirm their chemical composition and structural characteristics. Evaluations were conducted on the antioxidant and antimicrobial effectiveness of ZnO-PIP NPs through DPPH, ABTS, and MIC assays. Furthermore, cell viability and regulation of apoptotic gene expression on KB oral squamous carcinoma cells after the treatment were assessed to identify the potential anticancer properties of the ZnO-PIP NPs Results ZnO-PIP NPs showed significant antioxidant activity in both DPPH and ABTS assays, indicating their potential as potent scavengers of free radicals. The identified MIC of 50 μg/mL against dental pathogens highlighted the strong antimicrobial properties of ZnO-PIP NPs. Moreover, interaction analysis revealed the high binding affinity and complex amino acid interactions between ZnO-PIP NPs and receptors on dental pathogens. Additionally, in terms of anticancer activity, ZnO-PIP NPs exhibited a dose-dependent response against Human Oral Epidermal Carcinoma KB cells at concentrations ranging from 5 μg/mL to 100 μg/mL. Furthermore, ZnO-PIP NPs treatment increased the upregulation of crucial apoptotic genes, including BCL2, BAX, and P53, within the KB cells, unraveling the intricate mechanism of apoptotic activity. Conclusions This approach offers a multifaceted solution to combatting both oral infections and cancer, showcasing their potential for significant advancement in oral healthcare.

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Zinc oxide nanoparticles reduce biofilm formation, synergize antibiotics action and attenuate Staphylococcus aureus virulence in host; an important message to clinicians

Cited by 46 publications

References 40 publications

Green Synthesis of Zinc Oxide Nanoparticles Using Cymbopogon citratus Extract and Its Antibacterial Activity

Green Synthesis of Zinc Oxide Nanoparticles Using Cymbopogon citratus Extract and Its Antibacterial Activity

A combined treatment of Proteinase K and biosynthesized ZnO-NPs for eradication of dairy biofilm of sporeformers

Targeted piperine-coated zinc oxide nanoparticle induces the biofilm inhibition of dental pathogens and apoptosis of oral cancer through the BCL-2/BAX/P53 signaling pathway

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