Zinc oxide nanostructures as a control strategy of bacterial speck of tomato caused by Pseudomonas syringae in Egypt

Elsharkawy, Mohsen Mohamed; Derbalah, Aly; Hamza, Amany; El-Shaer, Abdelhamid

doi:10.1007/s11356-018-3806-0

Cited by 41 publications

(25 citation statements)

References 35 publications

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“…Zinc toxicity towards Pst was ascertained in vitro using several concentrations of zinc phosphate. Similar results were reported by Elsharkawy et al (2018) using zinc oxide nanoparticles and the agar disk diffusion technique. In particular, the EC 50 of 46 ppm zinc phosphate determined here in vitro was similar to the zinc concentration reached in the treated tomato plants (50 ppm), which was more than twice that of the control plants.…”

Section: Discussionsupporting

confidence: 89%

“…Pst growth was also repressed in the zinc phosphatetreated plants here. Similar findings have been reported for Pst in zinc-supplied plants (Elsharkawy et al 2018;Fones and Preston 2013) and for other pathogenic or beneficial bacteria (Othman et al 2017;Zhang et al 2015). This Effects of zinc phosphate treatment on zinc and phosphorous levels in tomato leaves.…”

Section: Discussionsupporting

confidence: 85%

“…We have also demonstrated that zinc is involved in the induction of plant defence responses, in terms of callose apposition and PR1b1 gene expression. In particular, the increased PR1b1 gene expression observed in the zinc phosphate-and ASM-treated plants is in agreement with Elsharkawy et al (2018) and Huang and Vallad (2018). For the PIN2 gene expression, in the three biological replicates we found that its expression was unchanged for both the zinc phosphate and ASM treatments, except for the reduction in the zinc phosphate-treated plants at the early treatment time (24 hpt).…”

Section: Discussionsupporting

confidence: 82%

“…This would indicate therefore that in the zinc phosphate-treated plants the diameters of the spots were lower than those for the ASMtreated plants. Similar data were obtained by Elsharkawy et al (2018) in the same pathosystem, who used zinc oxide nanoparticles as a spray treatment for the leaves.…”

Section: Discussionsupporting

confidence: 80%

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Zinc phosphate protects tomato plants against Pseudomonas syringae pv. tomato

et al. 2021

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The purpose of this study was to determine whether zinc phosphate treatments of tomato plants (Solanum lycopersicum L.) can attenuate bacterial speck disease severity through reduction of Pseudomonas syringae pv. tomato (Pst) growth in planta and induce morphological and biochemical plant defence responses. Tomato plants were treated with 10 ppm (25.90 µM) zinc phosphate and then spray inoculated with strain DAPP-PG 215, race 0 of Pst. Disease symptoms were recorded as chlorosis and/or necrosis per leaf (%) and as numbers of necrotic spots. Soil treatments with zinc phosphate protected susceptible tomato plants against Pst, with reductions in both disease severity and pathogen growth in planta. The reduction of Pst growth in planta combined with significantly higher zinc levels in zinc-phosphate-treated plants indicated direct antimicrobial toxicity of this microelement, as also confirmed by in vitro assays. Morphological (i.e. callose apposition) and biochemical (i.e., expression of salicylic-acid-dependent pathogenesis-related protein PR1b1 gene) defence responses were induced by the zinc phosphate treatment, as demonstrated by histochemical and qPCR analyses, respectively. In conclusion, soil treatments with zinc phosphate can protect tomato plants against Pst attacks through direct antimicrobial activity and induction of morphological and biochemical plant defence responses.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 80%

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Zinc phosphate protects tomato plants against Pseudomonas syringae pv. tomato

et al. 2021

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“…The growth of rice bacterial pathogens, B. glumae and B. gladioli, was inhibited significantly after the application of biogenic ZnONPs as compared to the control treatment. In the literature, various studies reported that ZnONPs effectively controlled the bacterial phytopathogens [34,50,51]. In this study, we described for the first time the antibacterial potential of biogenic ZnONPs against B. glumae and B. gladioli, which causes rice bacterial panicle blight disease.…”

Section: In Vitro Antibacterial Activitymentioning

confidence: 88%

Bioinspired Green Synthesis of Zinc Oxide Nanoparticles from a Native Bacillus cereus Strain RNT6: Characterization and Antibacterial Activity against Rice Panicle Blight Pathogens Burkholderia glumae and B. gladioli

Ahmed

Jiang

et al. 2021

Nanomaterials

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Burkholderia glumae and B. gladioli are seed-borne rice pathogens that cause bacterial panicle blight (BPB) disease, resulting in huge rice yield losses worldwide. However, the excessive use of chemical pesticides in agriculture has led to an increase in environmental toxicity. Microbe-mediated nanoparticles (NPs) have recently gained significant attention owing to their promising application in plant disease control. In the current study, we biologically synthesize zinc oxide nanoparticles (ZnONPs) from a native Bacillus cereus RNT6 strain, which was taxonomically identified using 16S rRNA gene analysis. The biosynthesis of ZnONPs in the reaction mixture was confirmed by using UV–Vis spectroscopy. Moreover, XRD, FTIR, SEM-EDS, and TEM analysis revealed the functional groups, crystalline nature, and spherical shape of ZnONPs with sizes ranging from 21 to 35 nm, respectively. Biogenic ZnONPs showed significant antibacterial activity at 50 µg mL−1 against B. glumae and B. gladioli with a 2.83 cm and 2.18 cm zone of inhibition, respectively, while cell numbers (measured by OD600) of the two pathogens in broth culture were reduced by 71.2% and 68.1%, respectively. The ultrastructure studies revealed the morphological damage in ZnONPs-treated B. glumae and B. gladioli cells as compared to the corresponding control. The results of this study revealed that ZnONPs could be considered as promising nanopesticides to control BPB disease in rice.

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