Zinc PVA versus potassium dichromate for preservation of microsporidian spores of human origin

El-Taweel, Hend A; Tolba, Mona M; Sadaka, H A; El-Zawawy, Lobna A; Osman, Mohamed

doi:10.1007/s00436-012-2888-8

Cited by 5 publications

(1 citation statement)

References 45 publications

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“…Acridine orange was added to achieve a final concentration of 0.05 mg/mL; the solution was left for 5 min before observation under a fluorescent microscope. The use of acridine orange for the viability assay of microsporidian spores have been reported previously (Peterson et al, 1988;El-Taweel et al, 2012). Stained spores (brightly fluorescent) in red were considered dead, and the proportion of dead/live spores was calculated for a minimum of 500 spores per sample.…”

Section: Assessing Spore Viabilitymentioning

confidence: 99%

Chemical Treatment of Beko Disease in <i>Seriola</i> Fishes: Laboratory Trials of Commercially Available Fishery and Veterinary Medicines

Yanagi¹,

Satoh

Mekata

et al. 2021

Fish Pathol.

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Beko disease in commercially farmed amberjacks, Seriola spp., has been known in Japan for many years (Egusa, 1982). The disease is caused by infection of the microsporidian Microsporidium seriolae, and forms unsightly whitish cysts to 1 cm length in fish somatic muscle. Within the cytoplasm of muscle cells, the parasite replicates and forms spores, so cysts may contain all of meronts, sporonts, sporoblasts, and spores (Egusa, 1982). As the infection matures the cysts disintegrate, resulting in characteristic concave depressions (beko) on the fish body surface.Historically, beko disease primarily afflicted juvenile Seriola quinqueradiata reared in restricted geographical areas. Also, most affected fish recovered by the time they reached harvest size (Sano et al., 1998;Yokoyama et al., 1996). Accordingly, M. seriolae was considered to have relatively low pathological effects on fish, and the unpleasant cysts found on a small proportion of harvested fish in a limited number of fish farms were not of great concern. However, since around 2012, outbreaks of beko disease have occurred in various localities

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Section: Assessing Spore Viabilitymentioning

confidence: 99%

Chemical Treatment of Beko Disease in <i>Seriola</i> Fishes: Laboratory Trials of Commercially Available Fishery and Veterinary Medicines

Yanagi¹,

Satoh

Mekata

et al. 2021

Fish Pathol.

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Potentiated anti-microsporidial activity of Lactobacillus acidophilus CH1 bacteriocin using gold nanoparticles

Mossallam

Amer

Diab

2014

Experimental Parasitology

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A new scope for orlistat: Effect of approved anti-obesity drug against experimental microsporidiosis

Abou-El-Naga¹,

Said²,

Gaafar³

et al. 2018

Medical Mycology

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As the current therapies for intestinal microsporidiosis are either inconsistent in their efficacies or hampered by several adverse effects, alternative antimicrosporidial agents are being sought. The present study is the first that was designed to evaluate the potency of orlistat, an approved anti-obesity drug, against intestinal microsporidiosis caused by both Enterocytozoon bieneusi and Encephalitozoon intestinalis. Results were assessed through studying fecal and intestinal spore load, intestinal histopathological changes, viability, and infectivity of spores from treated animals. Results showed that orlistat has promising antimicrosporidia potential, with better results in E. intestinalis than E. bieneusi. The animals that received orlistat showed statistically significant decrease in the fecal and intestinal spore load, when compared to the corresponding control infected nontreated mice. The results were insignificant compared to fumagillin and albendazole. Light microscopic examination of stained intestinal sections revealed amelioration of the pathological changes and decreased inflammatory cells detected in the control infected nontreated mice. Spores encountered from stool of orlistat-treated E. bieneusi and E. intestinalis mice showed low viability and significant reduction of infectivity versus their control. Thus, considering the results of the present work, orlistat proved its effectiveness against the intestinal microsporidial infection.

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Zinc PVA versus potassium dichromate for preservation of microsporidian spores of human origin

Cited by 5 publications

References 45 publications

Chemical Treatment of Beko Disease in <i>Seriola</i> Fishes: Laboratory Trials of Commercially Available Fishery and Veterinary Medicines

Chemical Treatment of Beko Disease in <i>Seriola</i> Fishes: Laboratory Trials of Commercially Available Fishery and Veterinary Medicines

Potentiated anti-microsporidial activity of Lactobacillus acidophilus CH1 bacteriocin using gold nanoparticles

A new scope for orlistat: Effect of approved anti-obesity drug against experimental microsporidiosis

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