Zinc-Rutin Particles Ameliorate DSS-Induced Acute and Chronic Colitis via Anti-inflammatory and Antioxidant Protection of the Intestinal Epithelial Barrier

Abstract

In patients suffering from inflammatory bowel diseases (IBDs), the immune system is disrupted and the intestinal barrier function is compromised. Here, six zinc-flavonoid particles were produced by one-step reaction via changing flavonoids (myricetin, quercetin, and rutin) and solvent (water and ethanol), and then their cytocompatibility and ability to scavenge H 2 O 2 , free radicals, and LPS-induced ROS were compared. Zinc-rutin particles (W-ZnRT) composed of rutin (78.92 wt %), Na 12 [ZnPO 4 ] 12 •12H 2 O (6.76 wt %), and crystal water were screened out because W-ZnRT exhibited 80.8 ± 15% cell viability against RAW264.7, could rapidly scavenge 78.1 ± 1% of H 2 O 2 and 71.6 ± 2% of DPPH within 30 min, and reduced LPS-increased intracellular ROS to normal levels. In addition, the therapeutic effects of rutin and W-ZnRT were also compared in dextran sulfate sodium (DSS)-induced acute and chronic colitis in mice. W-ZnRT was superior to rutin alone in chronic colitis (n = 9), although they were equally effective in acute colitis (n = 7). Compared to rutin, 11 oral doses of W-ZnRT (40 mg kg −1 ) significantly improved intestinal permeability (p = 0.0299) and colon length (p = 0.0025), reduced intestinal proinflammatory factors (IL-6, IL-1β, and TNF-α), and upregulated tight junction proteins to maintain intestinal barrier function. Taken together, these results identified W-ZnRT as an efficient and safe therapeutic strategy for IBD.

Engineered baicalein-decorated zinc phosphates for synergistic alleviation of inflammatory bowel disease by repairing the mucosal barrier and relieving oxidative stress

“…Therefore, it is common to observe the increased expression of both anti- and pro-inflammatory cytokines. 29–31 Accordingly, the level of IL-10 was also increased in LPS-induced RAW264.7. Both ZnBM and ZnPBS treatment significantly reduced the IL-10 level to the same as in the Control group (Fig.…”

Protective effect of zinc gluconate on intestinal mucosal barrier injury in antibiotics and LPS-induced mice

“…The present findings are consistent with this, demonstrating that the combined induction of antibiotics and LPS significantly decreased occludin gene and protein expression in the ileal tissues of mice. Furthermore, previous research suggests that zinc may positively regulate the expression of mucosal barrier proteins to produce inhibit inflammation, oxidative damage and promote epithelial cell repair (Souffriau et al, 2020;Wu et al, 2023). Yi et al showed that zinc oxide (ZnO) increased the height of ileal villi and enhanced the expression of Occludin in the duodenum and ileum, as well as increased the mRNA expression levels of MUC-2 and ZO-1 in the jejunum and ileum.…”