ZIP1 is a synaptonemal complex protein required for meiotic chromosome synapsis

Sym, Mary; Engebrecht, JoAnne; Roeder, G. Shirleen

doi:10.1016/0092-8674(93)90114-6

Cited by 593 publications

(664 citation statements)

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“…To define association of these proteins with meiotic chromosomes, spread nuclei were doublestained with antibodies to the myc epitope and to Zip1, the protein that makes up the transverse filaments of the synaptonemal complex (SC) (Sym et al 1993). The stage in meiotic prophase can be determined for any given nucleus based on the extent of SC formation (Fig.…”

Section: Association Of Mei4 and Rec114 With Meiotic Chromosomesmentioning

confidence: 99%

Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

et al. 2007

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In most sexually reproducing organisms, meiotic recombination is initiated by DNA double-strand breaks (DSBs) formed by the Spo11 protein. In budding yeast, nine other proteins are also required for DSB formation, but roles of these proteins and interactions among them are poorly understood. We report here further studies of the behaviors of these proteins. Consistent with other studies, we find that Mei4 and Rec114 bind to chromosomes from leptonema through early pachynema. Both proteins showed only limited colocalization with the meiotic cohesin subunit Rec8, suggesting that Mei4 and Rec114 associated preferentially with chromatin loops. Rec114 localization was independent of other DSB factors, but Mei4 localization was strongly dependent on Rec114 and Mer2. Systematic deletion analysis identified protein regions important for a previously described two-hybrid interaction between Mei4 and Rec114. We also report functional characterization of a previously misannotated 5′ coding exon of REC102. Sequences encoded in this exon are essential for DSB formation and for Rec102 interaction with Rec104, Spo11, Rec114, and Mei4. Finally, we also examined genetic requirements for a set of previously described two-hybrid interactions that can be detected only when the reporter strain is induced to enter meiosis. This analysis reveals new functional dependencies for interactions among the DSB proteins. Taken together, these studies support the view that Mei4, Rec114, and Mer2 make up a functional subgroup that is distinct from other subgroups of the DSB proteins: Spo11-Ski8, Rec102-Rec104, and Mre11-Rad50-Xrs2. These studies also suggest that an essential function of Rec102 and Rec104 is to connect Mei4 and Rec114 to Spo11.

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Section: Association Of Mei4 and Rec114 With Meiotic Chromosomesmentioning

confidence: 99%

Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

et al. 2007

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“…TF proteins, have therefore been identified independently in only a limited number of species, namely mammalian species (SYCP1) (Meuwissen et al, 1992), budding yeast (Zip1) (Sym et al, 1993), Drosophila (C(3)G) (Page and Hawley, 2001) and Caenorhabditis (Syp-1 and Syp-2) (Colaia´covo et al, 2003;MacQueen et al, 2002). Despite their lack of amino acid sequence similarity, SYCP1, Zip1 and C(3)G have a similar structure: they are long coiled coil proteins with globular domains at both ends.…”

Section: Transverse Filament Proteins Are Essential For Crossing Overmentioning

confidence: 99%

“…In Caenorhabditis and Drosophila meiotic crossing over is completely abolished (Colaia´covo et al, 2003;MacQueen et al, 2002;Page and Hawley, 2001), and in mouse almost completely (de Vries et al, 2005). Yeast zip1 null mutants on the other hand, still display about 30% of the wildtype level of crossing over (Sym et al, 1993).…”

Section: Transverse Filament Proteins Are Essential For Crossing Overmentioning

confidence: 99%

Meiotic Transverse Filament Proteins: Essential for Crossing Over

Heyting

2005

Transgenic Res

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Meiosis is a specialized set of two nuclear divisions, meiosis I and II, by which a diploid cell produces four haploid daughters. After premeiotic DNA replication, homologous chromosomes pair and recombine, and then disjoin at meiosis I. Subsequently, at meiosis II, the sister chromatids of each chromosome segregate. In nearly all eukaryotes, meiotic chromosome pairing culminates in the formation of a ladderlike supramolecular protein structure, the synaptonemal complex (SC) (Page and Hawley, 2004). The rungs of the ladder are known as transverse filaments (TFs). Genes encoding TF proteins have been identified in a limited number of organisms, and their function has been studied by mutational analysis. Although TF proteins show little amino acid sequence conservation, their structure and function are largely conserved. In all analyzed species, TF proteins are required for meiotic reciprocal recombination (crossing over).

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“…The chromatin rearin meiotic prophase cells (Meuwissen et al, 1992). If, rangements of meiotic prophase are accompanied by as seems likely, rnSCP1 is functionally homologous to the assembly and disassembly of synaptonemal comZip1p of yeast (Sym et al, 1993;Sym and Roeder, plexes (SCs) 2 (reviewed by von Wettstein et al, 1984Wettstein et al, ). 1995, it probably has a function in the regulation of 1 To whom correspondence should be addressed at Department of reciprocal crossing over and chromosome segregation Genetics, Agricultural University, Dreyenlaan 2, NL-6703 HA Wa- (Sym and Roeder, 1994 served domains within the protein and obtain a better mouse SCP1; hamsyn1a, hamster SCP1; rnSCP1, rat SCP1; hsSCP1, insight into the importance of structural features and human SCP1; SSC, standard saline citrate (150 mM NaCl, 15 mM amino acid sequence motifs in SCP1.…”

Section: Introductionmentioning

confidence: 99%

“…hsSCP1 and rnSCP1 have 75% amino these components were the putative TF proteins Zip1p acid identity. The most prominent structural features of yeast (Sym et al, 1993;Sym and Roeder, 1995) and and amino acid sequence motifs of rnSCP1 have been conserved in hsSCP1. Most probably, hsSCP1 is func-synaptonemal complex protein 1 (SCP1) of the rat (in tionally homologous to rnSCP1.…”

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confidence: 99%

Human Synaptonemal Complex Protein 1 (SCP1): Isolation and Characterization of the cDNA and Chromosomal Localization of the Gene

et al. 1997

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Human synaptonemal complex protein 1 (SCP1): isolation and characterization of the cDNA and chromosomal localization in the gene Meuwissen, R.L.J.; Meerts, I.; Hoovers, J.M.N.; Leschot, N.J.; Heyting, C. Published in: Genomics DOI:10. 1006/geno.1996.4373 Link to publication Citation for published version (APA):Meuwissen, R. L. J., Meerts, I., Hoovers, J. M. N., Leschot, N. J., & Heyting, C. (1997). Human synaptonemal complex protein 1 (SCP1): isolation and characterization of the cDNA and chromosomal localization in the gene. Genomics, 39, 377-384. DOI: 10.1006/geno.1996 General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. SCs consist of two proteinaceous axial cores or lateral Synaptonemal complexes (SCs) are structures that elements (LEs), one along each homolog, that are conare formed between homologous chromosomes (homo-nected along their length by numerous transverse fillogs) during meiotic prophase. They consist of two pro-aments (TFs); a third longitudinal structure, the centeinaceous axes, one along each homolog, that are con-tral element (CE), exists on the TFs, between both LEs nected along their length by numerous transverse fil- (Gillies, 1975; Schmekel et al., 1993). aments (TFs). The cDNA encoding one majorTo analyze the function of SCs, we have started to component of TFs of SCs of the rat, rnSCP1, has restudy their composition. Several protein components of cently been isolated and characterized. In this paper SCs of rodents (Heyting et al., 1987(Heyting et al., , 1989; Smith and we describe the isolation and characterization of the Benavente, 1992;Chen et al., 1992) and yeast (recDNA encoding the human protein homologous to viewed in Roeder, 1995) have been identified. Among rnSCP1, hsSCP1. hsSCP1 and rnSCP1 have 75% amino these components were the putative TF proteins Zip1p acid identity. The most prominent structural features of yeast (Sym et al., 1993;Sym and Roeder, 1995) and and amino acid sequence motifs of rnSCP1 have been conserved in hsSCP1. Most probably, hsSCP1 is func-synaptonemal complex protein 1 (SCP1) of the rat (in tionally homologous to rnSCP1. The hsSCP1 gene was this paper referred to as rnSCP1) (Meuwissen et al., assigned to human chromosome 1p12-p13 by fluores-1992). The cDNA encoding rnSCP1 was isolated and cence in situ hyb...

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ZIP1 is a synaptonemal complex protein required for meiotic chromosome synapsis

Cited by 593 publications

References 55 publications

Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

Meiotic Transverse Filament Proteins: Essential for Crossing Over

Human Synaptonemal Complex Protein 1 (SCP1): Isolation and Characterization of the cDNA and Chromosomal Localization of the Gene

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