Zirconia-incorporated zinc oxide eugenol has improved mechanical properties and cytocompatibility with human dental pulp stem cells

Jun, Soo Kyung; Kim, Hae‐Won; Lee, Hae‐Hyoung; Lee, Jung Hwan

doi:10.1016/j.dental.2017.09.021

Cited by 12 publications

(7 citation statements)

References 35 publications

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“…The depth of cure was determined by dividing the length by two ( n = 5). After each resin specimen ( ϕ = 10 mm and h = 6 mm) designated for extraction was light-polymerized by the aforementioned methodology without polyethylene film in between, Vickers hardness (HM-221, Mitutoyo, Tokyo, Japan) was measured at 500 gf (4.90 N) for 20 s on a cross-sectioned plain polished with up to 4000 grit SiC paper at every 0.5 mm increment ( n = 3, measurement) from the top of the surfaces (0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, and 6 mm) to the end of the three different specimen [28]. A total of 9 values from each group were recorded.…”

Section: Methodsmentioning

confidence: 99%

Depth-Dependent Cellular Response from Dental Bulk-Fill Resins in Human Dental Pulp Stem Cells

Lee

Sooyoun

Kim

et al. 2019

Stem Cells International

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The proper choice of dental composite resins is necessary based on the minimal cytotoxicity and antiodontogenesis on human dental pulp stem cells for dental pulp-dentin tissue repair and regeneration. The aim of this study was to evaluate the cytotoxicity and antidifferentiation effects of dental bulk-fill resins, able to be polymerized as a bulk status for filling deep cavity of a tooth by single light curing, against human dental pulp stem cells (hDPSCs) from three compartments corresponding to depth (0-2, 2-4, and 4-6 mm) from the light-curing site. Three bulk-fill composite resins (SDR, Venus bulk-fill (VBF), and Beautifil Bulk Flowable (BBF)) and a conventional flowable composite resin (Filtek Z350 XT flowable restorative (ZFF)) were individually filled into a cylindrical hole (h = 2 mm, Ф = 10 mm), and three compartments (total ~6 mm of height) were combined as a single assembly for light curing. The resin samples from the three layers were separated and eluted in the culture medium. The extracts were exposed to hDPSCs, and cytotoxicity and differentiation capability were evaluated. Depth of cure and surface hardness according to depth were determined. All bulk-fill resins except BBF revealed cytotoxicity from 4 to 6 or 2 to 4 mm, while ZFF was cytotoxic at over 2 mm. Depth of cure was detected from 3.55 to 4.02 mm in the bulk-fill resins (vs. ~2.25 mm in conventional resin), and 80% hardness compared with that of a fully polymerized top surface was determined from 4.2 to 6 mm in the bulk-fill resin (vs. 2.4 mm in conventional resin). Antidifferentiation was revealed at a depth of 4-6 mm in the bulk-fill resin. There was a difference in depth of cytotoxicity and antidifferentiation between the bulk-fill composite resins, which was mainly due to different cure depths and ingredients. Therefore, careful consideration of choice of bulk-fill resins is necessary especially for restoration of deep cavities for maintaining the viability and differentiation ability of dental pulp stem cells.

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Section: Methodsmentioning

confidence: 99%

Depth-Dependent Cellular Response from Dental Bulk-Fill Resins in Human Dental Pulp Stem Cells

Lee

Sooyoun

Kim

et al. 2019

Stem Cells International

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“…Eugenol combine with zinc oxide is used in filling dental cavities. [5,6] Additional to eugenol, cloves contain acetyl eugenol, betacaryophyllene, and vanillin. Cloves also contain some nonvolatile substances such as tannins (10-13%), triterpenes (2-4%), sterols, and flavonoids such as isobiflorin and biflorin.…”

Section: Research Articlementioning

confidence: 99%

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Das¹

2018

IJGP

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Objective: This work is designed to study and evaluate the antibacterial potency of phytochemicals from Syzygium aromaticum (clove) contrary to foodborne pathogens. In the present research, the extract of S. aromaticum was characterized using thin-layer chromatography, gas chromatography-mass spectrometric (GC-MS), and Fouriertransform infrared (FT-IR). Methods: The extraction was done by hydrodistillation process and the existence of essential oils was detected by analytical and chromatographic techniques. The conformation of the presence of eugenol in the extracted sample was achieved with high-performance thin-layer chromatography in comparison with the standard. The extract was subjected to GC-MS and FT-IR for quantification and structure prediction, respectively. Antibacterial assay was performed with different concentration of clove extracts (25%, 50%, and 75%) against different foodborne pathogens such as Escherichia coli, Listeria monocytogenes, Bacillus cereus, Staphylococcus aureus, Klebsiella oxytoca, and Staphylococcus epidermidis. ANOVA analysis with single factor was carried out to determine the significance of procured results. Results: The existence of higher concentration of eugenol along with caryophyllene and alpha-caryophyllene in the clove extract was manifested with GC-MS analysis. FT-IR analysis determined the major functional moieties and also confirmed the structure of bioactive compounds. After 24 h, the clove extract has shown to have antibacterial activity against all bacteria used in this study except for S. epidermidis. P value was found to be <0.05 which revealed the statistical significance of results. Conclusion: From this investigation, it is appraised that clove extract using the organic solvent dichloromethane has effective antibacterial property over foodborne pathogens. This study illustrates that organic compounds present in S. aromaticum could be an potent barrier against the microorganism tested and also could be used for the advancement in developing different drugs in Pharma industries.

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“…Its main compound is zinc oxide, considered as the solid phase of cement, and it is usually mixed with small quantities of MgO, which favors the deactivation of zinc oxide particles in the sintering step. As a consequence, this process leads to a retarding of the setting reaction of the cement and a liquid phase, which contains phosphoric acid and water 10,11 , which brings an acidic pH to the medium and to exhibit an exothermic reaction. This cement can damage the pulp tissues that remain in contact with the cement; it is not a good choice for clinical applications 12 .…”

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confidence: 99%

“…The nano-hydroxyapatite (nHA), has been used effectively in medical and dental applications rates due to its high biocompatibility and bioactivity. It benefits the development of bone tissue and the formation of a strong bond between the surface of the nHA and the tissue; therefore, it may be a safe alternative for pulp repair as it has a similar chemical structure as a human tooth made of apatite 11,13 while the bioactivity of nHA favors the formation of dentin by the deposition of a superficial layer of apatite in the presence of pulp fluids. This deposition assists maintain the integrity of the pulp tissue and induce a faster and more efficient formation of dentin 3 .…”

mentioning

confidence: 99%

Untitled

2020

IJPSR

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Zirconia-incorporated zinc oxide eugenol has improved mechanical properties and cytocompatibility with human dental pulp stem cells

Cited by 12 publications

References 35 publications

Depth-Dependent Cellular Response from Dental Bulk-Fill Resins in Human Dental Pulp Stem Cells

Depth-Dependent Cellular Response from Dental Bulk-Fill Resins in Human Dental Pulp Stem Cells

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