Zirconium for Sodium Peroxide Fusions

Petretic, G.J.

doi:10.1021/ac60056a042

Cited by 17 publications

(5 citation statements)

References 16 publications

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“…Enriched 99 Ru in metal powder form (99.11%) was purchased from Trace Sciences International (Richmond Hill, Ontario, Canada). A 99 Ru stock solution of 200 μg g –1 was prepared by dissolution of 99 Ru metal powder in sodium peroxide according to a previously reported procedure . Briefly, 20 mg of 99 Ru metal powder was mixed with 500 mg of sodium peroxide in a zirconium crucible and heated to 650 °C and held for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…A 99 Ru stock solution of 200 μg g −1 was prepared by dissolution of 99 Ru metal powder in sodium peroxide according to a previously reported procedure. 42 Briefly, 20 mg of 99 Ru metal powder was mixed with 500 mg of sodium peroxide in a zirconium crucible and heated to 650 °C and held for 10 min. Following the addition of 10 mL of 25% HCl, the mixture was heated to 110 °C and held for 30 min.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Protein Quantitation Using Ru-NHS Ester Tagging and Isotope Dilution High-Pressure Liquid Chromatography–Inductively Coupled Plasma Mass Spectrometry Determination

Liu

Hou

et al. 2012

Anal. Chem.

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An accurate, simple, and sensitive method for the direct determination of proteins by nonspecies specific isotope dilution and external calibration high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICPMS) is described. The labeling of myoglobin (17 kDa), transferrin (77 kDa), and thyroglobulin (670 kDa) proteins was accomplished in a single-step reaction with a commercially available bis(2,2'-bipyridine)-4'-methyl-4-carboxybipyridine-ruthenium N-succinimidyl ester-bis(hexafluorophosphate) (Ru-NHS ester). Using excess amounts of Ru-NHS ester compared to the protein concentration at optimized labeling conditions, constant ratios for Ru to proteins were obtained. Bioconjugate solutions containing both labeled and unlabeled proteins as well as excess Ru-NHS ester reagent were injected onto a size exclusion HPLC column for separation and ICPMS detection without any further treatment. A (99)Ru enriched spike was used for nonspecies specific ID calibration. The accuracy of the method was confirmed at various concentration levels. An average recovery of 100% ± 3% (1 standard deviation (SD), n = 9) was obtained with a typical precision of better than 5% RSD at 100 μg mL(-1) for nonspecies specific ID. Detection limits (3SD) of 1.6, 3.2, and 7.0 fmol estimated from three procedure blanks were obtained for myoglobin, transferrin, and thyroglobulin, respectively. These detection limits are suitable for the direct determination of intact proteins at trace levels. For simplicity, external calibration was also tested. Good linear correlation coefficients, 0.9901, 0.9921, and 0.9980 for myoglobin, transferrin, and thyroglobulin, respectively, were obtained. The measured concentrations of proteins in a solution were in good agreement with their volumetrically prepared values. To the best of our knowledge, this is the first application of nonspecies specific ID for the accurate and direct determination of proteins using a Ru-NHS ester labeling reagent.

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Section: Methodsmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Protein Quantitation Using Ru-NHS Ester Tagging and Isotope Dilution High-Pressure Liquid Chromatography–Inductively Coupled Plasma Mass Spectrometry Determination

Liu

Hou

et al. 2012

Anal. Chem.

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“…Sir: The findings of Petretic (4) and Dinnin (8) concerning the advantageous use of zirconium crucibles for sodium peroxide fusions have been confirmed in the laboratories of Strategic-Udy Processes, Inc. In many instances zirconium crucibles are superior to iron or nickel crucibles and afford a substitute for carbonate fusions in platinum; however, erroneous results have been noted in certain analyses.…”

Section: Use Of Zirconium Crucibles For Sodium Peroxide Fusionsmentioning

confidence: 99%

Use of Zirconium Crucibles for Sodium Peroxide Fusions

Anibal¹

1960

Anal. Chem.

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“…The use of zirconium crucibles for sodium peroxide fusions has been advocated by Petretic (1951) and Young and Strachan (1953). Experience in the rapid-analysis laboratory has verified their findings.…”

Section: Zirconium Cruciblesmentioning

confidence: 99%

Rapid analysis of chromite and chrome ore

Dinnin¹

1959

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Rapid methods for the determination of the major and of some minor constituents of chromite and chrome ore permit the complete analysis of a dozen or more samples to be performed in about 5 days with a precision comparable to that of the more conventional methods. The sample is fused with sodium peroxide in a zirconium crucible. The major constituents are determined from aliquots of a single solution of sample prepared by acidifying a water extraction of the fusion product. Cr2O3 is determined volumetrically by ferrous ammonium sulfate dichromate titration; SiOj is determined spectrophotometrically using the reduced silicomolybdate color; total iron is determined by a modified differential spectrophotometric method using orthophenanthroline. An additional aliquot, electrolyzed in a mercury cathode apparatus, is used for the titration of MgO with Versene, and for the spectrophotometric determinations of A12O3 and TiOj with alizarin redS and Tiron, respectively. The minor constituents are determined on another aliquot from which CrO^C^ has been volatilized by fuming with HCIC^ and HC1; MnO, V2Os, and NiO are then determined spectrophotometrically using the permanganate, phosphotungstovanadate, and a-furildioxime colors, respectively. Separate portions of sample are used for the determination of CaO by Versene titration, H2O by the Penfield tube method, and FeO by one or more methods. The precision of the method was tested by comparing the results of the rapid procedures with those obtained by conventional procedures on standard materials.

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Zirconium for Sodium Peroxide Fusions

Cited by 17 publications

References 16 publications

Protein Quantitation Using Ru-NHS Ester Tagging and Isotope Dilution High-Pressure Liquid Chromatography–Inductively Coupled Plasma Mass Spectrometry Determination

Protein Quantitation Using Ru-NHS Ester Tagging and Isotope Dilution High-Pressure Liquid Chromatography–Inductively Coupled Plasma Mass Spectrometry Determination

Use of Zirconium Crucibles for Sodium Peroxide Fusions

Rapid analysis of chromite and chrome ore

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