Ziziphus spina-christi leaf extract attenuates mercury chloride-induced testicular dysfunction in rats

Almeer, Rafa; Albasher, Gadah; Kassab, Rami B.; Ibrahim, Shaimaa R.; Al-Otibi, Fatimah O.; Alarifi, Saud; Ali, Daoud; Alkahtani, Saad; Moneim, Ahmed E. Abdel

doi:10.1007/s11356-019-07237-w

Cited by 22 publications

(12 citation statements)

References 54 publications

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“…Negative effects of mercury on reproductive traits in male rats include impairment of spermatogenesis, decrease in spermatozoa motility, and increase in pathological changes [ 171 , 172 , 173 , 174 , 175 , 176 , 177 ].…”

Section: Mercury (Hg)mentioning

confidence: 99%

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Effects of Cadmium, Lead, and Mercury on the Structure and Function of Reproductive Organs

Massányi

Madeddu

et al. 2020

Toxics

146

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Reproductive organs are essential not only for the life of an individual but also for the survival and development of the species. The response of reproductive organs to toxic substances differs from that of other target organs, and they may serve as an ideal “barometer” for the deleterious effects of environmental pollution on animal and human health. The incidence of infertility, cancers, and associated maladies has increased in the last fifty years or more, while various anthropogenic activities have released into the environment numerous toxic substances, including cadmium, lead, and mercury. Data from epidemiological studies suggested that environmental exposure to cadmium, lead, and mercury may have produced reproductive and developmental toxicity. The present review focused on experimental studies using rats, mice, avian, and rabbits to demonstrate unambiguously effects of cadmium, lead, or mercury on the structure and function of reproductive organs. In addition, relevant human studies are discussed. The experimental studies reviewed have indicated that the testis and ovary are particularly sensitive to cadmium, lead, and mercury because these organs are distinguished by an intense cellular activity, where vital processes of spermatogenesis, oogenesis, and folliculogenesis occur. In ovaries, manifestation of toxicity induced by cadmium, lead, or mercury included decreased follicular growth, occurrence of follicular atresia, degeneration of the corpus luteum, and alterations in cycle. In testes, toxic effects following exposure to cadmium, lead, or mercury included alterations of seminiferous tubules, testicular stroma, and decrease of spermatozoa count, motility and viability, and aberrant spermatozoa morphology.

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Section: Mercury (Hg)mentioning

confidence: 99%

“…Similar changes in testes were also confirmed after peroral mercury administration. Authors found a decline in spermatozoa, disorganization and degeneration of some spermatogenic cells and vacuolated areas within the seminiferous tubules in the mercury experimental group [ 175 ].…”

Section: Mercury (Hg)mentioning

confidence: 99%

Effects of Cadmium, Lead, and Mercury on the Structure and Function of Reproductive Organs

Massányi

Madeddu

et al. 2020

Toxics

146

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“…In the current study, treatment with SL and FS ameliorate CsA-induced renal injury evident by reduction of oxidative stress and enhancement of the antioxidant status. This could be referred to their ability to upregulate the gene expression of various antioxidant enzymes suggesting their renal protective role (Almeer et al, 2020;Omar, 2018;Parikh et al, 2019;Rafa et al, 2019). Moreover, α-linolenic acid in flaxseed could mediate triggering of this antioxidant status against renal damage (Omar, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…(Rafa et al, 2019). Phytochemical screening of sidr revealed the presence of many bioactive compounds such as flavonoids, essential oils, tannins, phytosterols, triterpenoid, vitamin C, glycoside, saponins, glycoside, and alkaloids (Almeer et al, 2020). Sidr is commonly used in the treatment of pain and inflammatory-related problems in conventional medicine in the gulf region and other nations (Ads et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Fortification of biscuit with sidr leaf and flaxseed mitigates immunosuppression and nephrotoxicity induced by cyclosporine A

2021

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The focus of consumers in healthy food turned to the possible health benefits of particular foods and food ingredients. This study aimed to evaluate the newly fortified biscuits supplemented with sidr leaves and flaxseed and to highlight their nutritional quality and health benefits against cyclosporine A-induced dexterous effects.Sidr leaves (SL), and flaxseed (FS) were used in the preparation of fortified biscuits.Proximate analysis and sensory evaluation were carried out on the biscuits. In in vivo study, 15 male albino mice were used for each group. Groups were divided into control, CsA, SL, FS, and SL+FS-treated groups. Hematological analysis, kidney function tests, oxidative stress, and anti-oxidant status were estimated. Flow cytometry was utilized to detect apoptosis and autophagy levels. The enzyme-linked immunosorbent assay (ELISA) was used for detection of interleukin-2 (IL-2), interferonγ (IFNγ), and transforming growth factor β1 (TGF-β1) levels. The composition of biscuits complemented by SL and FS demonstrated significant improvement in the nutritional value represented by the increase in overall protein, crude fat, crude fiber, ash, and carbohydrate contents. Treatment with SL and FS restored the disturbance in hematological, kidney function, oxidative, and antioxidant biomarkers. CsA-induced apoptotic and autophagic renal cell death was suppressed. Cytokines and pro-inflammatory markers were ameliorated. The use of SL and FS in dietary products can be recommended as a functional food. Moreover, they showed renal-protective, antioxidant, anti-inflammatory, and immune-enhancing activities. Practical applicationsSidr leaves (SL) and flaxseed (FS) were used in the preparation of fortified biscuits.The composition of biscuits complemented by SL and FS demonstrated a significant improvement in the nutritional values represented by the increase in overall protein, crude fat, crude fiber, ash, and carbohydrate contents. SL and FS showed a potential therapeutic activity in reversing CsA-induced dexterous side effects by acting as an antioxidant, antiapoptotic, antiautophagic, anti-inflammatory, renal-protective, and How to cite this article: El Seedy GM, El-Shafey ES, Elsherbiny ES. Fortification of biscuit with sidr leaf and flaxseed mitigates immunosuppression and nephrotoxicity induced by cyclosporine A.

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Luteolin protects against testicular injury induced by lead acetate by activating the Nrf2/HO‐1 pathway

et al. 2020

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Lead (Pb) is a toxic heavy metal that is harmful to humans, especially male reproductive organs. Luteolin (LUT) is a naturally occurring flavonoid with numerous biological activities. Our aim was to investigate the possible reproprotective effect of LUT against testicular deficits induced by Pb intoxication. In the present study, 28 rats were distributed into 4 groups: control, LUT (50 mg/kg), lead acetate (PbAc, 20 mg/kg), and LUT + PbAc groups, in which rats were pre‐treated with LUT 3 hr before PbAc injection. All animals were treated for 7 days. Oxidative stress, inflammatory and apoptotic markers along with histopathological changes have been examined using spectrophotometric, ELISA, real‐time PCR, and histopathological methods. PbAc injection elevated Pb concentration in testicular tissue and decreased levels of sex hormones. PbAc intoxication exacerbated lipoperoxidation and nitric oxide formation, depleted superoxide dismutase, and catalase activities along with glutathione and its originated enzymes (glutathione peroxidase and glutathione reductase). At the molecular level, PbAc deactivated nuclear factor erythroid 2‐related factor 2 and heme oxygenase‐1 in the testicular tissue. In addition, PbAc toxicity induced inflammatory and apoptotic cascades in testicular tissue as evidenced by the increased tumor necrosis factor‐alpha, interleukin‐1 beta, inducible nitric oxide synthase, Bax, and caspase 3, while Bcl‐2 was declined. Histopathological examination of testicular tissue also revealed that PbAc caused degeneration alterations in spermatogenic cells, the spermatogenic epithelial cells were disconnected from the basement membrane, and the seminiferous tubules were vacuolated. Remarkably, pre‐treatment with LUT minimized significantly the testicular damage induced by PbAc. Therefore, we conclude that LUT may have a beneficial effect against PbAc‐induced testicular injury through preventing oxidative challenge, inflammation, and finally apoptosis.

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Ziziphus spina-christi leaf extract attenuates mercury chloride-induced testicular dysfunction in rats

Cited by 22 publications

References 54 publications

Effects of Cadmium, Lead, and Mercury on the Structure and Function of Reproductive Organs

Effects of Cadmium, Lead, and Mercury on the Structure and Function of Reproductive Organs

Fortification of biscuit with sidr leaf and flaxseed mitigates immunosuppression and nephrotoxicity induced by cyclosporine A

Luteolin protects against testicular injury induced by lead acetate by activating the Nrf2/HO‐1 pathway

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